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The analysis of DNA isolated from different types of probiotic products using real-time PCR and HRM analysis
Sedláková, Lucie ; Rittich, Bohuslav (referee) ; Trachtová, Štěpánka (advisor)
The aim of this diploma thesis was to introduce real-time PCR with high-resolution melting analysis for Bifidobacterium species. Currently a small number of publication, dealing with identification of Bifidobacterium species using high-resolution melting analysis, is available. According to publications dealing with identification of lactic acid bacteria were selected primers P1V1 and P2V1, LAC1 and LAC2, LsppUPF and LsppUPR, V3F and V3R, V6F and V6R. Using this primers bacterial DNA was amplified by real-time PCR with high-resolution melting analysis. After evaluation of the measured results efficiency of selected primers was verified on DNA izolated from complex sample of probiotic product. After further optimisation real-time PCR with high-resolution melting analysis could be suitable using selected primers for Bifidobacterium species.
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Organic farming in the Czech Republic (geographic analysis)
SEDLÁKOVÁ, Lucie
The aim of this thesis is processing ÚZEI data for organic agricultural production in individual regions of the Czech Republic in the form of maps and graphs that provide a basic overview of the territorial deployment of crop and livestock production of organic farming. For crop production are given the following information: sown area in the conversion period and in the full organic mode, organic production and the average yield. Data of livestock in organic farming provide information about the numerical status of the individual categories of livestock. Part of the thesis is also devoted to the realization of crop and livestock organic products on the market in 2012. The thesis is part of the project Gaju 019/2013 / S.
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Výcvik Irského setra pro mysliveckou praxi
Sedláková, Lenka
In bachelor's work are described the most important parts of training Irish Setter aim to use for hunting. The training is divided according to hunting examinations, which can the owners with their dogs participate in. Temperament and exterior of the breed, history of breeding and hunting with setters and the conditions for breeding in Czech Republic are cha-rakterized. Also are coverd with writing my own experiences with training irish setter.
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Rizika internacionalizačního procesu MSP při vstupu na čínský trh
Sedláková, Lucie
The aim of the diploma thesis is to identify the key risk factors in the process of internationalization of small and medium-sized enterprises, thus the process of penetrating into the Chinese market. The thesis includes analysis of the current state in the Chinese market, complemented by a survey. Based on the results of the research, key risks and barriers influencing Czech SMEs in entering the Chinese market were identified. Also measures to these risks and the typical barriers were proposed to help businesses eliminate them.
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Natural sources of apian pasture and biocide agents
SEDLÁKOVÁ, Lucie
In this bachelor´s work I am trying to emphasize importance of bee´s grazing. A bee is an inseparable and indispensable part of countryside. In the first part there is an outline of economical and ecological significance of "Apis mellifera". Further I deal with description and introduction of pollen and its importance in nutrition of bees, pollen wimples, honey-giving plants, nectar and nectar-giving plants and also honeydew and its producers. Then I write about indicators of quality of bee´s grazing. In the next chapters there is description of sources of bee´s grazing during all the year according to phenological calendary. The second part is dedicated to poisoning of bees and how it is possible to protect bees.
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Programmes of support for small and medium-sized enterprises and their application in a chosen company
SEDLÁKOVÁ, Lucie
Small and medium-sized enterprises play an important role in creating new jobs and are the driving force of innovation. Therefore, their support is one of the priorities of the European Union. The aim of this study was to review treatment programs support their application for small businesses and processing applications for the development of selected small business. The chosen company was selected program support Progres, under which the company could be granted a subordinated loan for the purchase of tangible fixed assets. The main benefit of this project would be to speed up the production process, material savings and cost savings for service.
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