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Effects of n-3 polyunsaturated fatty acids, rosiglitazone, low caloric diet and environmental pollutants: obesity and related disorders
Kuda, Ondřej ; Kopecký, Jan (advisor) ; Bendlová, Běla (referee) ; Pravenec, Michal (referee)
4. CONCLUSIONS Conceming the specific aims of the thesis, the following conclusions may be formulated: l.Additionalbeneficia|effectsofthecombinedfieatmentbyn_3PUFAof marine origin and anti-diabetic drug rosiglitazone on obesity and related disorders were proven in mice fed high-fed diet. In comparison with either n- 3 PUFA or TZD effects alone, the combined treatment was more potent in amelioration of the following adverse pheÍrotypes: a. body weight, body weight gain and body lipid content; b. dYsliPidaemia; c. muscle insulin resistance demonstrated as enhanced muscle glycogen synthesis; obesity-associated inflammation of adipose tissue; and inaddition,additiveinductionofadiponectinwasfound,especiallyits multimeric form, which induces insulin sansitivity' These results on mouse modet suggest possible application for human medicine, where fish oil concentrates may enhance efficacy ofthe treatment byTZD,and reduce the risk of the adverse effect of the TzD-therapy' 2. Relatively specific induction oflipid oxidation by n-3 PUFA in the intestine was found, which may be important for e'g' anti-obesity and hypolipidemic effects ofthese liPids. 3. Changes in adiponectin concentration as well as distribution of its multimeric forms as a result oflow caloric diet were found in human patients' 4. Association between...
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Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes
Mihola, Ondřej ; Trachtulec, Zdeněk (advisor) ; Munclinger, Pavel (referee) ; Pravenec, Michal (referee)
Summaryofpublications 1. Positionalcloningof the Hybridsterility1 gene:finegenetic mappingand evaluationof twocandidategenes Hybridsteri|iýis one of the mechanismsof speciation.The Hybridsteriliý1 (HsÍí)genewasthefirstmappedmammaliangene.The geneaffectsfertiliýof male hybridsbetweencertainlaboratorystrains(suchas C57Bl/10)and Mus musculus musculusmiceby causinga breakdownof spermatogenesisat thestageof primary spermatocytes.|ntheprocessof positiona|c|oningoftheHsÍígene,Wegenerateda contigof bacterialartificialchromosomes(BACs)and subsequentlya lowcoverage sequenceof the candidateregionof the '12951/SvlmJstrain.New geneticmarkers narroweddown the HsÍíregionfrom 580 to 360 ki|obases.The productsof two genesfromthisregion,TATA-bindingprotein(Ibp)and proteasomesubunitbeta1 (Psmb1\,accumulateduringspermatogenesis.The proteinshave been described previouslyas having conservedC{erminal sequencesand species-specificN- termini.We eva|uatedthe candidacyof thesegenesfor Hsťíby a||e|icsequencing and by real-timereverse-transcriptionPCR of testicularmRNAs.The resultssuggest thatneitherthePsmÓí northe lbp genecausehybridsteri|ity.Thesing|enuc|eotide po|ymorphisms(SNPs)we havefound,was usedfor the hap|oýpeana|ysisof the HsÍíregion.
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