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Endless Cave
Pospíšil, Petr ; Pečiva, Jan (referee) ; Milet, Tomáš (advisor)
The goal of this thesis is to implement an application showing an endless cave. The basis of this cave is simplex noise method. On the noise produced by this function is afterwards applied thresholding. Produced grid of points is used like input for marching tetrahedrons algorithm. This algorithm converts volumetric data to boundary representation. Phong reflection model and Bump mapping were used in the application, too, in order to improve the visual quality. The application is based on OpenGL library. The first part of the technical report contains theoretical description of mentioned methods, the second part contains description of implementation.
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Influence of Wind Farm Integration to Power System
Pospíšil, Petr ; Belatka, Martin (referee) ; Malý, Jiří (advisor)
This bachelor’s thesis is divided into four main parts. The first point includes a characterization of the origin of wind, a principle and a division of the wind – mill motors and a characterization of a wind power plant. The second part analyses effects which the wind power plans have on the power system and also analyses grid problems with large amount of installed wind – mill motors. In the other point national grid codes are analysed (such as Germany, Denmark, Ireland). On the bases of these analyses the suggestions of the modifications of the Czech distribution grid code are summarized.
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Fluorescence Spectroscopy: Advanced methods and their defined applications in protein science
Pospíšil, Petr ; Hof, Martin (advisor) ; Heřman, Petr (referee) ; Polívka, Tomáš (referee)
The hydration and dynamics of the biomolecules appear to be vital for their proper biological functioning. In the presented thesis, various fluorescence techniques were developed and applied to access these properties and their changes upon the mutual interactions of the biomolecules. Initially, the solvent relaxation method based on recording time-dependent fluorescence shift (TDFS) was used to map DNA interactions with proteins and lipids by the newly synthesised fluorene dye covalently bound to the DNA. Secondly, copper-transporting ATPase was probed by Badan attached to the copper-binding cysteine-proline-cysteine motif. The variations in hydration were found to be crucial for the proper ATPase function. Third, a detailed study on quenching of Badan/Prodan fluorescence by tryptophan revealed the limitations of the TDFS method for protein studies, which is essential finding for further applications of TDFS. Fourth application involves investigations of heavy atom effects on the excited state relaxation processes by up-conversion approach in iodinated metallocorroles, which are promising dyes for biological imaging. The obtained findings shall help in further tuning of the optical properties of the corroles desired for the variety of applications. Finally, fluorescence correlation spectroscopy...
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Self-assembly and excited energy transfer in bacteriochlorophyll aggregates
Pospíšil, Petr ; Pšenčík, Jakub (advisor) ; Vácha, František (referee)
Bacteriochlorophyll c (BChl c) belongs among photosynthetic pigments. It is found in lightharvesting complexes of green photosynthetic bacteria in the form of self-assembled aggregates. It is possible to prepare BChl c aggregates also in vitro in aqueous solutions, in the presence of certain non-polar molecules (e.g. lipids or carotenoids). In this work, artificial light-harvesting antenna was prepared composed of BChl c and selected azulene derivative. The aggregation of BChl c with azulene enhances the absorption in the near UV region compared to BChl c. Fluorescence spectroscopy was used to study an efficiency of energy transfer from azulene to BChl c. The procedure used was based on fluorencence excitation spectra measurement, and a comparison with absorption 1-T spectra. The measurements were complicated by anomalous fluorencence of azulene, which occurs from the S2 state. Furthermore, the reference diode in the fluorescence spectrometer did not correct properly the signal in the near UV region, where azulene absorbs. The problem was partially solved by preparing the new correction files using selected laser dyes. Excitation spectra demonstrate the presence of the energy transfer from azulene to BChl c, however, it was not yet possible to satisfactorily quantify its efficiency.
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Variances in the Blue Card Directive Transposition across the European Union
Pospíšil, Petr ; Young, Mitchell (advisor) ; Šlosarčík, Ivo (referee)
This work attempts to analyze causes of divergent transposition of the Blue Card directive, regulating the conditions of immigration of highly-skilled workers from third countries, between individual EU Member States. The differences are analyzed on two levels. Firstly, in terms of timeliness and correctness of transposition, secondly, from a perspective of a different manner of transposition of the discretionary clauses entailed in the Blue Card directive (whether the Member States opted for an open or for a restrictive way of transposition of these provisions). Scholarly literature about transposition compliance and highly-silled immigration policies creates the foundation, by means of which the author identifies several variables and articulates hypotheses aiming at providing explanation of the divergent transposition of the directive in the respective Member States. The author classifies the Member States in groups and clusters according to their transposition performance and its results embody a point of departure for a subsequent analysis. From each group, certain number of countries is selected for a detailed country-level analysis in order to ensure universality and geographical representativeness of the outcomes. To review how the individual hypotheses confirmed in the Member States, the...
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Means of expression in film noir and their use in the following development of world cinematography
Pospíšil, Petr ; SMUTNÝ, Vladimír (advisor) ; MACÁK, Jiří (referee)
This thesis describes the origins of film-noir genre, historical context of classic noir era as well as the influence of its film predecessors, emphasising the tradition of german expressionism. Each single aspect creating the characteristic film-noir look is analysed and described in detail, and illustrated using film stills containing such aspect. Following text summarises reasons and circumstances of the end of this classic era. The second part of my thesis is dedicated to the utilization of this noir aspects in later films all the way up to the present times. This part is also supplemented with practical examples of these elemets being used.
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Fluorescence Spectroscopy: Advanced methods and their defined applications in protein science
Pospíšil, Petr ; Hof, Martin (advisor) ; Heřman, Petr (referee) ; Polívka, Tomáš (referee)
The hydration and dynamics of the biomolecules appear to be vital for their proper biological functioning. In the presented thesis, various fluorescence techniques were developed and applied to access these properties and their changes upon the mutual interactions of the biomolecules. Initially, the solvent relaxation method based on recording time-dependent fluorescence shift (TDFS) was used to map DNA interactions with proteins and lipids by the newly synthesised fluorene dye covalently bound to the DNA. Secondly, copper-transporting ATPase was probed by Badan attached to the copper-binding cysteine-proline-cysteine motif. The variations in hydration were found to be crucial for the proper ATPase function. Third, a detailed study on quenching of Badan/Prodan fluorescence by tryptophan revealed the limitations of the TDFS method for protein studies, which is essential finding for further applications of TDFS. Fourth application involves investigations of heavy atom effects on the excited state relaxation processes by up-conversion approach in iodinated metallocorroles, which are promising dyes for biological imaging. The obtained findings shall help in further tuning of the optical properties of the corroles desired for the variety of applications. Finally, fluorescence correlation spectroscopy...
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Limiting Factors of the European Union Counter-Terrorism Mechanisms
Pospíšil, Petr ; Weiss, Tomáš (advisor) ; Šlosarčík, Ivo (referee)
The basis, upon which this thesis has been built, are the two following trends: occurence of terrorist attacks in the EU and creation of new counter-terrorism institutions on the EU level. A frequency of both trends has been rising: the occurence of terrorist attacks on the EU territory has increased despite the closer cooperation in security and counter-terrorism area that can manifested by adopting new measures and designing new institutions. Therefore, the author asks a question: What factors are hindering a better functioning of the EU counter-terrorism mechanisms? The six most significant EU counter-terrorism institutions with exclusively or overwhelmingly counter-terrorism mandate is subjected to analysis. The respective limits of their functioning have been subsumed to a classification that was created by the author using the general institutional theory, in particular the theory of regimes. The attention is given to an interplay between the EU counter-terrorism bodies and their counterparts on the national level. In case of every institution that has been explored, the author evaluates the following criteria. Firstly, presence of a "patterned behaviour" in sense of cooperation between the national counter-terrorism bodies and the EU institutions. Secondly, a subjective perception of such...
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Managing Users as a Source of Risk
Pospíšil, Petr ; Konečný, Martin (referee) ; Sedlák, Petr (advisor)
This diploma thesis focuses on human resources mainly in Critical information infrastructure and Important information systems. Thesis focuses on the most frequent threats for users and design possible model of threat reduction. Integral part of results is designing of effective security awareness education program according to the Law on Cyber Security.
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