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The relationship of young citizans of Prague to cottaging
Pirochová, Klára ; Pauknerová, Karolína (advisor) ; Moravcová, Mirjam (referee)
The diploma thesis focuses on the phenomenon of young Prague residents' cottaging in the present day. The author defines cottaging as all activities taking place in a dwelling in the country that was not originally built for recreational purposes. The thesis is founded on her own ethnographic survey based on the method of semi-structured interviews, namely 30 interviews conducted with cottagers for the purpose of this thesis. Mental mapping and participant observation constitute additional methods. The focal point of the project lies not only in the collection of interviews and mental maps with the cottagers, but mainly in further analysing and interpreting the collected data. The thesis also focuses on the historical development of cottaging from the 19th century to the present. Looking for the reasons and motivations that have contributed to the development and popularity of cottaging and how these motivations have changed is an important point. In her work, the author focuses on sharing the premises of the cottage with relatives and on the performance of specific work and leisure-time activities. Among other things, the author concludes that the presumption that the opening of the borders after the fall of the Iron Curtain and the possibility of travelling abroad would result in a decline in...
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Targeted analysis of eicosanoids by LC/MS
Moravcová, Marie ; Kuda, Ondřej (advisor) ; Kozlík, Petr (referee)
Eicosanoids represent large group of biologically active lipid metabolites syntesized from polyunsaturated fatty acids that play an important role in many physiological processes. To describe complex metabolism of these compounds, analytical methods including extraction from a biological sample using solid phase extraction and liquid chromatography coupled with mass spectrometer detection were used. Solid phase extraction of biological samples was optimized on four types of reverse phase columns of which column Strata X 60 mg/3 ml, 33 um (Phenomenex , USA) was the most effective. Also, alternative solid phase extraction of eicosanoids using columns with ion exchange sorbents and a column with normal phase were tested, but proved to be unsuitable for targeted analysis of eicosanoids. The extraction method yielding the best results - Strata X 60 mg/3 ml, 33 µm (Phenomenex, USA) was used for the separation of eicosanoids from mouse gonadal fat samples. Eicosanoids were analyzed by liquid chromatography and the separation mechanisms were tested on three UPLC core-shell columns of different lengths (50 mm, 100 mm, 150 mm). The most effective separation of prostaglandin E2 and prostaglandin D2 was achieved using the longest column Kinetex 150 mm × 2,1 mm, 2,6 µm. Furthermore, ionization parameters, such...
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