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Different ways of usage of "directed" and "undirected" seriality shown on an example of spectator consumption of soap opera Ulice
Kvasničková, Eva ; Reifová, Irena (advisor) ; Bednařík, Petr (referee)
The thesis called "Different ways of usage of "directed" and "undirected" seriality shown on an example of spectator consumption of soap opera Ulice" is composed of two main sections. In the first one the theoretical pieces of information about seriality and serial narratives are summarized. Foremost the focus is directed to the two dominant types - series and serial- not only in the perspective of narrative theory but also within the frame of English written literature about serial narratives. The thesis also deals with the genre called soap opera, with its history and some of the current hybrid genres. The soap opera viewers are characterized, as well as their relationship with the characters. Also the concept of "directed" and "undirected" seriality is introduced. It could be explained that within the "directed" seriality the external distributor of narrativity exist while in the case of "undirected" seriality it is the viewer himself who is in charge of managing seriality. In the practical part of this thesis the qualitative anylysis and grounded theory are used to examine two groups of spectators of a popular Czech soap opera Ulice (The Street) - first group consists of television viewers (who use the "directed" seriality) and the second group is composed of internet viewers (who use the "undirected"...
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Study of the role of glutathione in the anthracycline-induced cardiomyocyte injury
Vávrová, Anna ; Šimůnek, Tomáš (advisor) ; Kvasničková, Eva (referee)
The clinical usefulness of anthracycline antineoplastic drugs has been limited by the risk of serious cardiotoxicity. Its precise mechanisms have not been fully elucidated, although the oxidative stress is widely believed to play a principal role. The anthracycline toxicity to heart may result from its high accumulation in myocardial tissue, richness on mitochondria producing reactive oxygen species and relatively poor antioxidant systems including superoxide dismutase, catalase and glutathione peroxidase. This study is focused on the role glutathione in daunorubicin (DAU)-induced cardiotoxicity both in vitro and in vivo. Total, reduced and oxidized glutathione (GSH/GSSG) have been determined by a spectrophotometric method using dithiobis(2-nitrobenzoic acid) in DAU (0.1 - 10 µM) - exposed H9c2 cardiomyoblast cell line and heart tissue from daunorubicin-treated rabbits (3mg/kg weekly, 10 weeks). In H9c2 cells, DAU induced significant toxicity (IC50=0.48 µM), levels of total glutathione were slightly but insignificantly decreased, the levels of oxidized glutathione were unafected. Neither buthionine sulfoximine (BSO, an inhibitor of glutathione synthesis) nor 2-oxo-4-thiazolidine-carboxylic acid (OTC, a glutathione synthetic precursor) had any significant effects of DAU cytotoxicity. This contrasted...
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Apoptosis and its role in selected human diseases
Černá, Hana ; Kvasničková, Eva (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Hana âerná Supervisor: Prof. RNDr. Eva Kvasniãková, CSc. Title of diploma thesis: Apoptosis and its role in selected human diseases Apoptosis, which represents a specific form of programmed cell death, is an essential physiological process. It is very important for the organism's developement and for the maintenance of tissue homeostasis. It is a highly regulated process that is always triggered by a definite signal. This signal activates specific apoptotic pathways, a series of mechanisms that subsequently lead to cellular decline. The dysregulation of apoptosis is associated with a variety of pathological conditions such as cancer, some disorders of the immunty system or of the cardiovascular system, or neurological diseases. To the last mentioned belong neurodegenerative disorders that are characteristic of the decline of selective neuronal populations. Examples of these are Alzheimer's and Parkinson's disease. Although its pathogenesis is still not fully elucidated, many studies indicate that the loss of neurons could be tightly related to excessive apoptotic activity. In spite of some discrepancies apoptosis can therefore be expected to an important part of the pathogenesis of these...
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Zkoumání účinku CTA1R7K-ChrA-DD způsobujícího toleranci na BDC2.5 CD4 buňky vyvolávající diabetes
Kadová, Zuzana ; Wsól, Vladimír (advisor) ; Kvasničková, Eva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Kadová Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Title of diploma thesis: Investigations of tolerogenic effects of CTA1R7K-ChrA-DD on diabetogenic BDC2.5 CD4 cells This project was focused on the study of a novel tolerance inducing vaccine, CTA1R7K-ChrA-DD. It was investigated if this construct can inhibit autoimmune diabetes and if the CTA1R7K-ChrA-DD treatment can affect proliferation and cytokine production of BDC2.5 CD4 cells. The treatment with CTA1R7K-ChrA-DD was effective only one time of two repeated experiments (using the same protocol) when the mice received the dose of construct more times on the other hand when they were treated only once the treatment was without effect. After in vitro restimulation with the PS3 peptide it would be expected less INF gamma production and less proliferation in CTA1R7K-ChrA-DD treated mice but in most cases, we got the opposite result. This study incites hope for that CTA1R7K-ChrA-DD construct actually has ability to induce protection against diabetes and is a good start for further studies.
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Different ways of usage of "directed" and "undirected" seriality shown on an example of spectator consumption of soap opera Ulice
Kvasničková, Eva ; Reifová, Irena (advisor) ; Bednařík, Petr (referee)
Diploma thesis "Different ways of usage of "directed" and "undirected" seriality shown on an example of spectator consumption of soap opera Ulice" is composed of two main sections. In the first one the theoretical pieces of information about seriality and serial narratives are summarized. Foremost the focus is directed to the two dominant types - series and serial - not only in the perspective of narrative theory but also within the frame of English written literature about serial narratives. The thesis also deals with the genre called soap opera, with its history and some of the current hybrid genres. The soap opera viewers are characterized, as well as their relationship with the characters. Also the concept of "directed" and "undirected" seriality is introduced. It could be explained that within the "directed" seriality the external distributor of narrativity exist while in the case of "undirected" seriality it is the viewer himself who is in charge of managing seriality. In the practical part of this thesis the qualitative anylysis and grounded theory are used to examine two groups of spectators of a popular Czech soap opera Ulice (The Street) - first group consists of television viewers (who use the "directed" seriality) and the second group is composed of internet viewers (who use the "undirected"...
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Scheme " Heath city"
Valčíková, Šárka ; Wsól, Vladimír (advisor) ; Kvasničková, Eva (referee)
1028 inhabitants of the town Kroměříž were investigated in the project "Healthy Town" in the years 2005, 2006 and 2007. Using acquired data, some epidemiological characteristics of region's inhabitants were evaluated and important experiences were put into preventive clinical practice. The experiences are following: 1. The project "Healthy Town" is not a pilot study. Contact centers are attended by citizens who pay attention to active interest of their health. In this population women and senior citizens dominate, many of them have health problems and the incidence of cardiovascular factors considerably exceeds "the average population". The study has shown that 37% of them are overweight and 13% are obese. 16% of inhabitants have hypertension of over 140/90 mm Hg. 57% suffer from lipid metabolism failure and 14% suffer from hyperglycemia. 2. Then we observed a relationship between BMI index and investigated high risk markers (systolic and diastolic pressure, serum concentration of total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides and glucose). We have proved a significant relation between the degree of overweight and values of blood pressure and concentration of triglycerides, and on the other hand we have discovered a statistically insignificant relation between total cholesterol...
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Studies on carbonyl reductases 1 and 3 variants with emphasis on S-nitrosoglutathione as substrate and inactivator
Hartmanová, Tereza ; Wsól, Vladimír (advisor) ; Kvasničková, Eva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Tereza Hartmanová Supervisor: Dr. Claudia Staab, Dr. Hans-Jörg Martin, Prof. Ing. Vladimír Wsól, Ph.D. Title of the diploma thesis: Studies on carbonyl reductases 1 and 3 variants with emphasis on S-nitrosoglutathione as substrate and inactivator Human CBR1 and CBR3 (carbonyl reductases 1 and 3) are monomeric, NADPH-dependent enzymes belonging to the short chain dehydrogenase/reductase superfamily. Although they are highly similar at the amino acid level (72 % identity) the enzymes exhibit considerable differences in substrate specificity. The CBR1 substrate spectrum is well described, including a variety of compounds e.g. the endogenous indol isatin, S-nitrosoglutathione (GSNO), prostaglandins, quinones, and many carbonyl group bearing xenobiotics. In contrast, CBR3 shows a distinct and much narrower range of substrates and its role is still not fully clarified. Nevertheless, the dissimilar substrate spectra strongly indicate that CBR1 and CBR3 play different metabolic roles. In the present study, the catalytic properties of CBR1 towards the latest CBR1 substrate described, GSNO, were investigated. CBR3 was assessed for potential GSNO-reducing activity, but no in vitro activity was...
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