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The Origin of the Marian type called Regina in Bohemian territory
Kolářová, Pavlína ; Klípa, Jan (advisor) ; Hlaváčková, Jana Hana (referee)
This task is dealing with a question of origin of a Marian type called Regina and its possible precursors in a bohemian painting of 2/2. 14th century. The base of the research is a critical reflexion of existing theories. The crucial part is a comparison of documented Marian images from the Augustinian monastery in Roudnice nad Labem with the final type of Madonna and its later variants. Except for typological, formal and iconographical analysis is worked with a historical and archival resources and their interpretation. The second important part is an introduction to a terminology base of a theme and its critical reflection, mostly focused on a problem of unreflected using of terms in older texts. Keywords Regina, Madonnas of Roudnice type, Marian images, Madonnas of 2/2 14th century, Madonna from Roudnice, Madonna from Březnice, Madonna of Augustinians in Krakow, Grace Madonnas
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Obnova druhově bohatých luk na výsypkách po těžbě uhlí, vliv růyných technik a velikosti obnovované plochy
Kolářová, Petra ; Frouz, Jan (advisor) ; Mudrák, Ondřej (referee)
Common problems and characteristics of sites affected by the mining are land occupation, pollution, land degradation, hydrologic changes and landscape change what belong to the major environmental problems in many countries. Despite the regulation prescribed by the Mining Act which brought significant progress in post mining land reclamation, emphasise mostly in recovery of production capability of landscape, many recent studies state it may not be so effective in restoration of diversity and ecosystem services. In order to properly assess newer approaches in spoil heaps recultivation our research is focused on the restoration of species rich meadows using near-nature methods, specifically the transfer of turves and seeds-containing hay transfer supported by management (mowing and mowing with mulching). Additionally, the research is focused on influence of the plot size on restoration success and on effect of management on soil development. Both of the applied near-nature methods of species rich meadow recultivation are effective in species provisioning and thus succession acceleration. More successful is the method of transferred turves where it was possible to establish more than 60% of the species from the donor site. The species richness on sites and its spreading is dependent on implemented...
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HPLC determination of silybin diastereoisomers in plasma of laboratory animals
Kolářová, Petra ; Tesařová, Eva (advisor) ; Bednář, Petr (referee)
Silybin is the main component of silymarin, a standardized extract obtained from the seeds of milk thistle (Silybum marianum). Flavonolignan silybin has antioxidant, hepatoprotective, chemoprotective and antitumor activities. Natural silybin occurs as an approximately equimolar mixture of two diastereoisomers - silybin A and silybin B. Analytical separation of these diastereoisomers is possible but preparative separation is complicated. The biological activity of the silybin A is different from the silybin B. Silybin diastereoisomers are mainly conjugated to glucuronides and sulfates in organism. A mixture of both silybin diasteroisomers is used in the majority of reported biological, chemical and pharmacokinetic studies. For the first time, optically pure silybin A and silybin B were used for pharmacokinetic study in this thesis. The object of this work was determination of the concentration of free and total silybin in rats plasma in relation to time. Theoretical introduction describes the current state of the problem of chemistry, pharmacology and pharmacokinetics of silybin diastereoisomers. Second part is focused on the selection of appropriate analytical column, chromatographic method and suitable procedure for preparation of biological samples for the determination of the silybin...
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Possibilities to restore seminatiral meadows in post industrial sites.
Kolářová, Petra ; Frouz, Jan (advisor) ; Tátosová, Jolana (referee)
The post-industrial sites (brownfields) are sites which were devastated and abandoned by industrial production (generation areas, dumping grounds, mining areas…), but buildings and paved areas abandoned by agricultural production, too. On the other hand on these very disturbed places, primary succession, may leads to recovery of valuable ecosystems. It shows the potential of these areas for the restoration of valuable near natural ecosystems. One of the possibilities how to use these areas could became establishing species rich meadows rich, which could return to the meadow ecosystem his original function. They could benefit from oligotrophic status, at least some of these sites, too. The aim of this work is to summarize the information about factors which influence structure and diversity of meadows, their restoration and assess chance post-industrial sites utilization for the recovery of species rich meadows. Finally results of experiment about restoration of species-rich meadow ecosystems on mine spoil dumps in the surrounding of Sokolov are represented.
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Preparative diastereomeric separation of silybin and its sulfates by RP-HPLC
Kolářová, Petra ; Tesařová, Eva (advisor) ; Kalíková, Květa (referee)
Silymarin is standardized extract isolated from fruits of Milk Thistle plant (Silybum marianum). The principal component of silymarin is silybin. This flavonolignan is mainly responsible for the medicinal effects of Milk Thistle fruits: antioxidant, hepatoprotective, anticancer and chemoprotective activities. Natural silybin exists as an equimolar mixture of diastereomers A and B whose preparative separation is very hard. It was shown that the biological activity of silybin A and B are different. Silybin in the blood conjugates mainly to sulfates. The structure or biological activity of the sulfates is not yet known. The aim of this work is to develop practically applicable method for preparative separation of diastereomers of silybin A and B, and sulfates, which are considered as one of the major metabolites of silybin. The preparative method for separation of silybin A and B in the mobile phase consisting of 50% MeOH on the chromatographic column Labio C18 25x250 mm, was developed. In addition, preparative method for separation of mixture of products accured from the sulphation of silybin-23-acetate in the mobile phase consisting of MeOH/H2O 60/40 (v/v) with addition of 10 ml/l HCOOH, was optimized.
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"Self-portrait in the circles of smoke": self-fashioning of the modern artist as a smoker in the Czech art at the turn of the 19th and 20th century
Kolářová, Petra ; Konečný, Lubomír (advisor) ; Lahoda, Vojtěch (referee)
The topic of this Diploma Thesis is the representation of an artist as a smoker in the Czech Art at the turn of the 19th and 20th century. The motive is studied in the works of artists of the 1890s generation and the early avant-garde (Osma, Skupina výtvarných umělců), with the focus on the iconography of a smoker in art as well as the cultural history of smoking. Apart from paintings and graphics it uses other visual sources such as portrait photographs, caricatures published in contemporary newspapers and posters. The Thesis looks for a wider tradition in the European art and maps specific types of artist-smoker for whom cigarette, pipe or cigar is a part of his personal stylization. The first part concentrates on the early usage of the motive in the baroque painting and in the 19th century as such. The next part focuses on self-fashioning of modern artists characterized by their definition against social conventions. The attribute of smoking plays a role of a symbol of bohemian Iife, dandy astuteness, neurasthenic decadent, social or artistic revolt or emancipation of female-artists. The last part deals with the relationship of smoking to the actual art production of modern artists. Smoking is a metaphor for creativity of the symbolist poet or painter who draws the inspiration from his subjective...
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