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Roles of cytoskeleton in mouse polyomavirus trafficking
Klímová, Lucie ; Forstová, Jitka (advisor) ; Šmahel, Michal (referee)
6 Roles of cytoskeleton in mouse polyomavirus trafficking ABSTRACT: Mouse polyomavirus (mPyV) is small non-enveloped DNA virus. Its endocytic pathway is studied for a potential utilisation of polyomaviral virus-like particles in gene therapy and/or immunotherapy. mPyV enter cells by internalisation into smooth monopinocytic vesicles. During it's journey through the cell, it pass through early endosomes, and at the time 3 hours post infection, it is localised in endoplasmic reticulum and recycling endosomes. Many aspects of mPyV trafficking and nuclear entry are not clear yet. Time-lapse live imaging fluorescence confocal microscopy was used to describe the mouse polyomavirus intracellular movements. For these studies, we utilised mPyV fluorophore-labeled virions and cells expressing GFP-tagged g-actin or alpha-tubulin. Some virion-loaded vesicles were seen to move with actin organised into dynamic structures. Some of these structures resembled actin comets created by Listeria or vaccinia virus. At the same time post infection (40-60 min post infection), movement of the virion loaded vesicles along mirotubules was observed suggesting the simultaneous involvement of actin and tubulin during mPyV trafficking. Dynamitin, a dominant negative inhibitor of dynein-dynactin function reduced mPyV infection. Taken...
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Optimalization of child care outside the own family
KLÍMOVÁ, Lucie
Foster Currently, about foster care a lot of talk , especially in connection with changes in the new Civil Code. Placing the child in foster care for a child better than a residential facility. Children need parents (although they are foster parents). Foster parents can give a child the same love and care as biological parents and often better. It is important that the child at the foster family that can meet the children's needs as well as biological parents and a child to be respected and loved. The theoretical part consists of chapters, the first dealing with the family, the second chapter is devoted to institutional care and institutional care and the third chapter is devoted to foster care. The chapters about the process of arranging foster care, a child adapt to a new environment, preparation for the adoption of a child to the NDP, NDP support, dissimulation adoption. The last chapter focuses on contact with the child, which will be located in the care of the adoptive parents. The aim of the empirical part of this study was to explore the current system of child care outside their own family. For information on its work on the topic Optimizing child care outside their own family, he was elected a qualitative approach. The data were collected through narrative and polořízených interviews with selected respondents. Narrative and semi-controlled interviews were conducted by questions prepared in advance Selected respondents were foster parents, adoptive parents and staff of orphanages and children centers. The results of the interviews institutional facilities were compared among themselves and with specialized information resources The research has found that changes in the law brought a lot of negatives. Stay of children in institutions is longer. Research suggests that in facilities under the auspices of the Ministry of Health to manage the child into foster care. In plants under the auspices of the Ministry is usually fail. Further interviews revealed that even in foster care in the ER able to locate the child. From the device under the auspices of the Ministry of Education to conventional foster care and adoption not usually place a child. Only in one interview I was met with the answer that the facilities under the auspices of the Ministry of Education placed in the care of four children, was a sibling. The number of children out of institutions under the auspices of the Ministry of Health to adoption is higher than in conventional foster care. In international adoption is mostly not place any child of the above devices. The most common reason for the dismissal of the device under the auspices of the Ministry of Health is to return to the original family and placement in foster care. Children from the device under the auspices of the Ministry of Education are released because of the age and the completion of training. Furthermore, the interviews show that the device under the auspices of the Ministry of Health cares for more children than in institutions under the auspices of the Ministry of Education. ZDVOP is of great importance according to the staff of Children's centers and orphanages in the childcare system. Workers orphanages, child centers, adoptive parents and foster parents agreed that it would substitute parent should have the same characteristics as the biological parent. Foster parents and the staff of Children's centers and orphanages have the same idea about the characteristics of foster parents. Surrounding by foster parents responding to the decisionadoption of a child in foster care positively. Expectations foster parents are satisfied . Candidates for foster care should, as foster parents and adoptive parents should prepare for everything. Spare parent usually has the possibility to get acquainted with the child before they were handed over to the care.
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