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HPLC determination of silybin diastereoisomers in plasma of laboratory animals
Kolářová, Petra ; Tesařová, Eva (advisor) ; Bednář, Petr (referee)
Silybin is the main component of silymarin, a standardized extract obtained from the seeds of milk thistle (Silybum marianum). Flavonolignan silybin has antioxidant, hepatoprotective, chemoprotective and antitumor activities. Natural silybin occurs as an approximately equimolar mixture of two diastereoisomers - silybin A and silybin B. Analytical separation of these diastereoisomers is possible but preparative separation is complicated. The biological activity of the silybin A is different from the silybin B. Silybin diastereoisomers are mainly conjugated to glucuronides and sulfates in organism. A mixture of both silybin diasteroisomers is used in the majority of reported biological, chemical and pharmacokinetic studies. For the first time, optically pure silybin A and silybin B were used for pharmacokinetic study in this thesis. The object of this work was determination of the concentration of free and total silybin in rats plasma in relation to time. Theoretical introduction describes the current state of the problem of chemistry, pharmacology and pharmacokinetics of silybin diastereoisomers. Second part is focused on the selection of appropriate analytical column, chromatographic method and suitable procedure for preparation of biological samples for the determination of the silybin...
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Possibilities to restore seminatiral meadows in post industrial sites.
Kolářová, Petra ; Frouz, Jan (advisor) ; Tátosová, Jolana (referee)
The post-industrial sites (brownfields) are sites which were devastated and abandoned by industrial production (generation areas, dumping grounds, mining areas…), but buildings and paved areas abandoned by agricultural production, too. On the other hand on these very disturbed places, primary succession, may leads to recovery of valuable ecosystems. It shows the potential of these areas for the restoration of valuable near natural ecosystems. One of the possibilities how to use these areas could became establishing species rich meadows rich, which could return to the meadow ecosystem his original function. They could benefit from oligotrophic status, at least some of these sites, too. The aim of this work is to summarize the information about factors which influence structure and diversity of meadows, their restoration and assess chance post-industrial sites utilization for the recovery of species rich meadows. Finally results of experiment about restoration of species-rich meadow ecosystems on mine spoil dumps in the surrounding of Sokolov are represented.
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Preparative diastereomeric separation of silybin and its sulfates by RP-HPLC
Kolářová, Petra ; Tesařová, Eva (advisor) ; Kalíková, Květa (referee)
Silymarin is standardized extract isolated from fruits of Milk Thistle plant (Silybum marianum). The principal component of silymarin is silybin. This flavonolignan is mainly responsible for the medicinal effects of Milk Thistle fruits: antioxidant, hepatoprotective, anticancer and chemoprotective activities. Natural silybin exists as an equimolar mixture of diastereomers A and B whose preparative separation is very hard. It was shown that the biological activity of silybin A and B are different. Silybin in the blood conjugates mainly to sulfates. The structure or biological activity of the sulfates is not yet known. The aim of this work is to develop practically applicable method for preparative separation of diastereomers of silybin A and B, and sulfates, which are considered as one of the major metabolites of silybin. The preparative method for separation of silybin A and B in the mobile phase consisting of 50% MeOH on the chromatographic column Labio C18 25x250 mm, was developed. In addition, preparative method for separation of mixture of products accured from the sulphation of silybin-23-acetate in the mobile phase consisting of MeOH/H2O 60/40 (v/v) with addition of 10 ml/l HCOOH, was optimized.
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"Self-portrait in the circles of smoke": self-fashioning of the modern artist as a smoker in the Czech art at the turn of the 19th and 20th century
Kolářová, Petra ; Konečný, Lubomír (advisor) ; Lahoda, Vojtěch (referee)
The topic of this Diploma Thesis is the representation of an artist as a smoker in the Czech Art at the turn of the 19th and 20th century. The motive is studied in the works of artists of the 1890s generation and the early avant-garde (Osma, Skupina výtvarných umělců), with the focus on the iconography of a smoker in art as well as the cultural history of smoking. Apart from paintings and graphics it uses other visual sources such as portrait photographs, caricatures published in contemporary newspapers and posters. The Thesis looks for a wider tradition in the European art and maps specific types of artist-smoker for whom cigarette, pipe or cigar is a part of his personal stylization. The first part concentrates on the early usage of the motive in the baroque painting and in the 19th century as such. The next part focuses on self-fashioning of modern artists characterized by their definition against social conventions. The attribute of smoking plays a role of a symbol of bohemian Iife, dandy astuteness, neurasthenic decadent, social or artistic revolt or emancipation of female-artists. The last part deals with the relationship of smoking to the actual art production of modern artists. Smoking is a metaphor for creativity of the symbolist poet or painter who draws the inspiration from his subjective...
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"Self-portrait in the circles of smoke": self-fashioning of the modern artist as a smoker in the Czech art at the turn of the 19th and 20th century
Kolářová, Petra ; Lahoda, Vojtěch (referee) ; Konečný, Lubomír (advisor)
The topic of this Diploma Thesis is the representation of an artist as a smoker in the Czech Art at the turn of the 19th and 20th century. The motive is studied in the works of artists of the 1890s generation and the early avant-garde (Osma, Skupina výtvarných umělců), with the focus on the iconography of a smoker in art as well as the cultural history of smoking. Apart from paintings and graphics it uses other visual sources such as portrait photographs, caricatures published in contemporary newspapers and posters. The Thesis looks for a wider tradition in the European art and maps specific types of artist-smoker for whom cigarette, pipe or cigar is a part of his personal stylization. The first part concentrates on the early usage of the motive in the baroque painting and in the 19th century as such. The next part focuses on self-fashioning of modern artists characterized by their definition against social conventions. The attribute of smoking plays a role of a symbol of bohemian Iife, dandy astuteness, neurasthenic decadent, social or artistic revolt or emancipation of female-artists. The last part deals with the relationship of smoking to the actual art production of modern artists. Smoking is a metaphor for creativity of the symbolist poet or painter who draws the inspiration from his subjective...
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Is daily activity in known environment correlated with activity in experimental apparatus?
KOLÁŘOVÁ, Petra
This study was aimed at daily activity of common vole (Microtus arvalis). The features as trajectory and differences in body weight were measured. Trajectory during three days long activity were compared with trajectory measured in Open Field Test. The impact of gender was assessed and change in body weight was analysed. Values as trajectory, mobility and mean velocity were gauged in Phenotyper and Open Field test, measuring were done in EthoVision (Noldus). Analysing proved differences between genders in body weight change. Correlation between trajectories and impact of gender at daily activity were not proved.
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The history and present of the Moscow Metro
KOLÁŘOVÁ, Petra
The theme of this dissertation is the Moscow metro. A large part is devoted to the history and the actual construction, which was designed by one of the most important representatives of contemporary Moscow, Lazar Kaganovich. Through this work we will visit three architecturally interesting stations, go through the period of Second World War when the metro was hiding not only for the residents of Moscow, learn what is hidden under the name Metro 2 . Pay attention to some selected terrorist attacks and their consequences. At the very end, we will focus on the present and compare what happened over seventy-eight years of underground changed. This dissertation includes photos of metro stations.
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The mobbing and its interaction in the formation in the employment
KOLÁŘOVÁ, Petra
My thesis deals with the workplace violence as a serious risk factor of the daily life and work and it is a complex problem, especially when psychological violence at the work is a serious problem that is still very underrated. The harassment is the summary name that referred to very bad situation for everybody and it projects to the everyday situations at work and it is manifested not only in labor relations, but also on output and performance of individuals and whole. The thesis of the topic was chosen on the based my own experiences with the regards to the timeliness of this social problem. The main aim is to inform about the hidden and dangerous process of the psychological abuse in the workplace, many participants cannot solve it, because they are designed only for the consequences not the causes of the investigated phenomenon.
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Social - economy Function of Business on the Market of EU
KOLÁŘOVÁ, Petra
My thesis deals with the problems of the Fair Trade business not only on the market of the European Union but it deals with these problems on the market of the Czech republic. The preface deals with the question {\clqq} why did I choose this theme``. The Fair Trade is good exaple and hits off the subjekt of my work. The second part of my thesis is concentrated on the importance and the function of the business from the theoretical standpoint. First of all I define advantages of business and then I put it together with the determinated functions from the economic aspects.The third part of my thesis describes methods, that was used to achieve the determination aims of my thesis.I determined the social {--} economic function and the consequence of the Fair Trade business. The fourth part is the analytical part of my thesis. There is the analysis of the environment of the Fair trade in the teritory of the Czech Republic in the frame of the providing of services and a acquaintanceship this brend in our republic, because the Fair Trade is there still almost unknown and there aren´t datas of researches.The last part of my thesis contains the summary and the betterment this problems.
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Účetní závěrka v ČR
Kolářová, Petra ; Cardová, Zdenka (advisor) ; Lovas, Miroslav (referee)
The goal of my diploma thesis is to give a comprehensive interpretation on the issue of accounts compiled by entities in the Czech Republic. I describe the operations and activities before, during and after its compilation. I also briefly refer to International Financial Reporting Standards and the appreciation of assets and liabilities. Finally I'm doing an analysis of a final accounts of the real company.
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