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Protein chemistry and mass spectrometry in biochemical research
Pompach, Petr ; Bezouška, Karel (advisor) ; Chmelík, Josef (referee) ; Kovářová, Hana (referee)
Protein chemistry and mass spectrometry in biochemicul research Petr Pompach Ph. D. Thesis Department of Biochemistry Faculty of Science Charles University Supervisor: Doc. RNDr. Karel Bezouška, CSc. Prague 2006 tEia*iiitg;;tiBiÉ (n OO oO O\ O\ C.l C.l .+ : : : : :ÉGlc.l c.: Ý l.) a z t-r (J Fl E) lra J a U z a Fr tl5 a Fr zr\J) alai P.: ai a - Lr o L d a (|i iť r h t) o() r a a q C) ! - H F (n F íi a o) O a X, o >. C) o .l I +i oL o -O o o l.r U) N z oo - O o I L a Li +i vi ol Ei!t ,ql -l E 3a (n z E F a F z F z U pg ť: šé3; Ei ĚE$áE€EÉEáiz E ÉĚ E Ě s E ; u ; ó =-E5Ě:gd:'i = t Ei giĚg;;:1lV)iĚggĚgEsáEĚi* E Ě=;Éía:š;Éť E É!řěEEEFgg : ií€gáŤÉ5 ž:::š;;i : zi:€ i=ts€ĚiĚišĚÉiš;š5ĚiE.:. = = € .T ŽlĚE g;t$jíigggřEE iuEái E :É€šáĚ g;iÉ{Ě;ítÉÉF€Ě E iĚĚĚi ; š;šš!iiE řě E ši gE ig Ý E giĚE šgig;išiš::gígFĚi+g s E *'suĚšěĚ Ěie *Ěá, uE trE s'' -k*-=a.*:.* g: EPE€'šEEř€9 ěÍ.E ;š e::E;;áe;iÉgřšiE řĚt.E*E$*eE=$[:gř.v :IEF€=áfrE;='2E-ď ?! : g"HEu.:i=ĚE€;ÉE€ř€ Eš,*éEě9t.F;EE.Ě., ťiEEEE*ě:iiEĚgĚ:Éžáx;šš ř 5šEtsť uE.E.= e *n á:; € žEř: € ÉE.: i= ř-g p; i ĚĚ;: € i íEĚ = Hi;2E É*Ě u, Ě y ?' : 6 ě ř ; fij .: .c F=E;-sg&,Ě7Éď€3E€€ -i€E.E =:Í E=bÉ::ň !B:,: E t šáč š 3e = Ě l E ř f L Ei:í:9:*q;€gq3:;:.E: ťšÉI: Ě i ?E Ě ; Éi É.EE E EEE :!rEě45BEE EE.= €Eě íEEra-vĚ=3ň €.E3 iillťlgtššgtgi;g;ggiĚš; iáágá9 $*i;Ě:E; -Eás;t;É$ \o Ěi gi...
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Proteomics of biological fluids
Jarkovská, Karla ; Kovářová, Hana (advisor) ; Petrák, Jiří (referee) ; Ulčová-Gallová, Zdeňka (referee)
Reproductive diseases, mainly those resulting in the infertility affect the chances of human being to reproduce. On the contrary, the heart disease, cancer and degenerative diseases currently account for majority of deaths in the world. Usually, these lifestyle diseases need longer lifespan to become the cause of death. The proteins secreted by cells carry important information about the cell's well-being, as well as about the condition of the tissues formed by these cells. Once secreted, these proteins may also be transferred throughout the body by means of body fluids, many of which are easily accessible for further 'in-depth' studies. Cellular and secreted proteins are often a focus of studies using proteomic means and the revelation of protein alterations can lead us to new ideas about the molecular mechanisms of diseases as well as possible identification of proteins that may be used as new targets for pharmaceutical intervention or molecules that could be used for diagnostic or prognostic purposes. Taking into consideration the above aspects, this research was undertaken to find proteins that could: (a) characterise the human follicular fluid as microenvironment of the maturing oocyte, to increase understanding of reproductive processes to improve the techniques of assisted repro- duction;...
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Detached house with workshop
Janda, Lukáš ; Kovářová, Hana (referee) ; Utíkalová, Ivana (advisor)
Abstract Topic of this bachelors work is detached house with workshop in council Lhotky u Malotic. House is designed for 4-6 member family. Structure is without basement with one above ground floor, attic and loft. Workshop and garage are separate connected with main building by fence wall. Desing is based on traditional czech village architecture. Main living rooms are situated to the south part of garden. Land is availiable from distant local road. Vertical load bearing structure is mansory. Horizontal load bearing structures are from traditional timber beam ceiling. Staircase is cast-in-place reinforced concrete. Roof is classical saddle. Project was done in computer program ArchiCAD.
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Superior detached house
Malík, Ivan ; Kovářová, Hana (referee) ; Utíkalová, Ivana (advisor)
The bachelor’s thesis focuses on the design of a house with one housing unit, situated on a sloping terrain in the cadastral area of Brno - Židenice. The building has one floor above ground, there is a cellar under a part of the house, and the space is opened towards the west. A shed roof has been chosen. The layout of the building is divided into three sectors: resting, working and relaxing, all contributing to the above standard quality of the house.
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Monolithic reinforced concrete detached house
Oplíštil, Jakub ; Kovářová, Hana (referee) ; Sobotka, Jindřich (advisor)
The intention of Bachelor thesis is new building of monolithic house in the village Majlant. It is a detached building with two floors and a flat roof. The house will be used by a family of three. The main entrance to the building is situated at northwest side. Floor plan of the object is simple, the great design emphasis was on highlighting the south side of the building. This was accomplished by sliding the southern bearing elements above the wooden terrace and use large glazed surfaces. The view of this side of the builing has the S-shape. Interier is a combination of colored concrete and wood. Supporting structures are made of exposed monolithic reinforced concrete. Partitions are made of aerated concrete blocks. The staircase is made of wood. Theses includes parts of documentation and drawings, that are handled as detailed documentation in computer software AutoCAD.
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Detached house with project office
Váňa, Jan ; Kovářová, Hana (referee) ; Brzoň, Roman (advisor)
The subject of this bachelor’s thesis is a newly bulit family house with a design office. The building consists of two main parts. Both parts have its own entrance and are not interconnected. The first part of the building, a two storey family house, is designed for a family of four. The design office is a single-storey building intended for 6 employees and an owner of the building. The structure is based on strip foundations made of plain concrete. The walls and ceilings are made of the Heluz system. The perimeter walls are insulated with ETICS mineral wool insulation composite system. The building has a flat roof with an asphalt waterproofing system.
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Apartments "CORA" in the heart of the Highlands
Petrová, Miroslava ; Kovářová, Hana (referee) ; Sobotka, Jindřich (advisor)
This diploma thesis focuses on design of an accommodation guest house – apartments and stables. The apartment object has two above-ground floors and a partial basement. Two social rooms and a room for staff are located in the part of the basement that reaches above ground. The dinning room, sanitary facilities and apartments for people with reduced mobility are located on the first floor. Four apartments are located in a residential attic. The recreational building is made of ceramic bricks and has a gabled roof and counter skylights. The agricultural building will be used for stabling horses, parking agricultural machinery and storing hay and feed. Stables are designed as a timber frame construction with a supporting frame made of timber pillars coated with the Fermacell boards. The building has a gabled roof with a skylight above the horse boxes for ventilation. The thesis contains drawings and technical documentation and it is composed as an implementation project.
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Family house with housing units
Vejmělek, Lukáš ; Kovářová, Hana (referee) ; Sobotka, Jindřich (advisor)
The subject of this thesis work is the two floors house with housing units in the city of Brno - Lisen. The proposal is processed at the level of project documentation for building construction stage. Family house is located on plot no. 3278/92, cadastral area of Brno – Lisen. The building is without a basement on two floors of a wall system of brick blocks POROTHERM, based on the foundation walls and covered with a flat roof vegetation. There are three residential units, two of which used to rent.
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Mountain pension in Dolni Morava
Zajíček, Martin ; Kovářová, Hana (referee) ; Čupr, Karel (advisor)
The theme of bachelor’s thesis is new-building mountain boarding house in Dolni Morava. The building is set in flat terrain. It has two floors above ground. The building is designed of systém Porotherm and the ceiling construction of system Porotherm Strop. The roof strukture is a saddle roof with timber roof supported by load-bearing walls and with angle 34 degrees.
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Skeleton detached house
Studený, Marek ; Kovářová, Hana (referee) ; Sobotka, Jindřich (advisor)
The matter of the bachelor´s thesis is the new building of double-storey detached house in a local part of Kudlov the city of Zlin. The object is formed by reinforced concrete, prefabricated elements forming the skeleton structure. The supporting structure consists of continuous columns, beams, braces and all atypical sections. Ceiling, roof and foundation slab is designed from prestressed ceiling panels Spiroll. Building´s stair is from reinforced concrete, prefabricated. As the filler of peripheral walls and for realization of partition walls as well is used sand-lime bricks. The roof is flat, vegetation. The building has the shape of a cube and includes a built-in garage. The house is designed for housing a family of four. The main entrance and the entrance to garage is at the level of the first floor from the street Modra. The main living rooms are oriented to the southern side.
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