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Analysis of current views of mutism in specialized Czech and foreign literature
Páleníková, Petra ; Klenková, Jiřina (advisor) ; Květoňová, Lea (referee)
This diploma thesis deals with mutism from the perspective of specialized czech and foreign literature. The character of the thesis is theoretical and the thesis is divided into three parts. In the first chapter, there is mutism described based on the study of czech specialized literature as well as etiology, symptoms, diagnostics and treatment. The second chapter describes the same categories but based on the study of foreign literature. The last part is dedicated to the analysis and comparison of acquired knowledge in particular areas. The most signifiant differences can be found in the area of diagnostics and therapy of mutisms, which are more elaborated in foreign literature than in Czech literature. Some differences appear in all other areas of mutism. The learning theory is the characteristic feature of foreign literature in the field of etiology of mutism, also the social enxiety is the characteric feature in the area of symptoms and treatment; overall we can see a dominant influence of the Diagnostic and Statistical Manual of Mental Disorders. The Czech literature provides a number of different views not only on the definition of mutism, but also on the etiology and symptomatology. The diagnostics and the therapy of mutism is not further elaborated in the Czech literature.
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Regulation of transcription in mycobacteria.
Páleníková, Petra ; Krásný, Libor (advisor) ; Mrvová, Silvia (referee)
The bacterial cell has to be able to cope with environmental changes. Adaptation to these changes is achieved by changes in gene expression. Gene expression is regulated mostly at the level or transcription initiation. Transcription initiation depends on the sequence of promoters and is regulated by alternative sigma factors and many transcription factors acting either as activators or repressors. This work describes various ways of transcription regulation in the bacterial genus Mycobacterium that includes deathly pathogens such as M. tuberculosis and M. leprae. The typical characteristics of this genus are poorly conserved promoters, a high number of sigma and transcription factors, the presence of two-component systems and a lot of small RNAs that have not been characterized in detail so far.
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Regulation of expression of Ms1, a sRNA from Mycobacterium smegmatis
Páleníková, Petra ; Krásný, Libor (advisor) ; Lichá, Irena (referee)
Bacteria are exposed to various environmental conditions during their growth. They have to cope with rapid changes in temperature, lack of nutrition, etc. To survive, bacteria alter their gene expression. One type of regulation of gene expression is regulation by small RNAs (sRNAs). In bacteria, a well-studied sRNA is 6S RNA that binds to the RNA polymerase holoenzyme. However, 6S RNA has not been identified in several bacterial species. Mycobacteria are a genus that probably does not have 6S RNA. Instead, Mycobacterium smegmatis possess another sRNA - Ms1. Ms1 structurally resembles 6S RNA and indeed it was first identified as a 6S RNA structural homologue. However, Ms1 binds to RNAP devoid of any sigma factor, and, therefore, is significantly distinct from 6S RNA. This work describes regulation of expression of Ms1. DNA fragments of different length from the region upstream of the Ms1 gene were prepared. These fragments were fused to the lacZ reporter gene and their activity was tested in different growth phases and under stress. This allowed identification and characterization of the core promoter sequence and regulatory sequences that might interact with transcription factor(s). Promoter activity increased with increased length of the promoter fragment and after transition into stationary...
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Analysis of current views of mutism in specialized Czech and foreign literature
Páleníková, Petra ; Klenková, Jiřina (advisor) ; Květoňová, Lea (referee)
This diploma thesis deals with mutism from the perspective of specialized czech and foreign literature. The character of the thesis is theoretical and the thesis is divided into three parts. In the first chapter, there is mutism described based on the study of czech specialized literature as well as etiology, symptoms, diagnostics and treatment. The second chapter describes the same categories but based on the study of foreign literature. The last part is dedicated to the analysis and comparison of acquired knowledge in particular areas. The most signifiant differences can be found in the area of diagnostics and therapy of mutisms, which are more elaborated in foreign literature than in Czech literature. Some differences appear in all other areas of mutism. The learning theory is the characteristic feature of foreign literature in the field of etiology of mutism, also the social enxiety is the characteric feature in the area of symptoms and treatment; overall we can see a dominant influence of the Diagnostic and Statistical Manual of Mental Disorders. The Czech literature provides a number of different views not only on the definition of mutism, but also on the etiology and symptomatology. The diagnostics and the therapy of mutism is not further elaborated in the Czech literature.
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Regulation of transcription in mycobacteria.
Páleníková, Petra ; Krásný, Libor (advisor) ; Mrvová, Silvia (referee)
The bacterial cell has to be able to cope with environmental changes. Adaptation to these changes is achieved by changes in gene expression. Gene expression is regulated mostly at the level or transcription initiation. Transcription initiation depends on the sequence of promoters and is regulated by alternative sigma factors and many transcription factors acting either as activators or repressors. This work describes various ways of transcription regulation in the bacterial genus Mycobacterium that includes deathly pathogens such as M. tuberculosis and M. leprae. The typical characteristics of this genus are poorly conserved promoters, a high number of sigma and transcription factors, the presence of two-component systems and a lot of small RNAs that have not been characterized in detail so far.
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