|
| |
|
| |
|
| |
|
|
In vitro study of topical use of cidofovir
Sedláčková, Martina ; Vávrová, Kateřina (advisor) ; Kováčik, Andrej (referee)
Martina Sedláčková In vitro study of topical use of cidofovir Pharmaceutical faculty in Hradec Králové, Charles University Abstract Cidofovir (CDV), an antiviral drug belonging to the group of acyclic nucleoside phosphonates, is a highly potent drug used for treatment of various infections of viral origin. The low usage of CDV is caused by its price. This work deals with possibilities of increasing the concentration and availability of CDV r in the skin that could lead to lowering of the production costs of the preparations containing this substance. The goal of this work was to study the concentration of CDV in different skin layers after its application on intact and tape striped human skin. The effect of permeation enhancer 6-(dimethylamino)hexanoic acid dodecyl ester was also investigated. Both attitudes were investigated in vitro using modified Franz diffusion cells. Three different concentration of CDV (0.3%, 1% and 3%) were applied on the intact or tape-stripped skin with or without 1% enhancer. In intact skin, the combination of 3% CDV with 1% enhancer provided epidermal CDV concentration of 1115 µg/g, which is 1.45-times higher than without an enhancer. In tape-striped skin, the epidermal concentration of CDV increased to 2584 µg/g which is 1.55-times higher than without enhancer. The highest...
|
|
|
Stationary phase based on ionic liquids for separation of ibuprofen impurities
Komůrková, Lenka ; Kučera, Radim (advisor) ; Kastner, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Lenka Komůrková Supervisor: Doc. PharmDr. Radim Kučera, Ph.D. Title of diploma thesis: Stationary phase based on ionic liquids for separation of ibuprofen impurities. Rigorous thesis was focused on the determination of ibuprofen and its pharmacopoeial impurities B, E, F and J using gas chromatography coupled with mass spektrometry. The analysis was performer on a SLB-IL 100 capillary column with a stationary phase based on ionic liquid - 1,9-di(3-vinylimidazolium)nonane bis(trifluoromethy) sulfonyl imide. Ibuprofen was derivatized with alkyl chloroformate (isobutyl chloroformate, ethyl chloroformate, heptafluorochloroformate) in the presence of the appropriate alcohol (isobutanol, ethanol, heptafluorobutan-1-ol). The derivatization reaction was successful for all derivatizing agents used. However, the use of ethyl chloroformate appeared to be the best due to the negligible amount of non-derivatized ibuprofen residue. Subsequently, the amount of added alcohol, the derivatization of ibuprofen depending on the retention of the sample in the ultrasonic bath and the influence of the ibuprofen solution on the derivatization and subsequent analysis were investigated....
|
|
|
Feature-based multi-resolution topography
Brůha, Lukáš
A three-dimensional (3D) representation of the topographic surface is an important element in planning, civil engineering and mapping. Many state-of-the-art representa- tions provided graphical, 3D model of entire planet. However, the existing solutions to 3D topographic surface lack the geometric flexibility and accuracy on boundaries with models of other geographic features. Most of the contemporary approaches to digital Earth solutions focus on the visualization performance. The high visual performance is achieved by the use of special data structures optimized for rendering. However, this optimization towards visualization hampers the data management of spatial data, their analysis and distribution. Therefore, the introduced solution reflects on multiple requirements of digital Earth systems. In addition to the visualization performance, the requirements regarding data interoperability, data management and distribution, data analysis and the multiple level of detail (LOD) are considered as essential for the design of the new solution. The topographic surface is central to the proposed method. It provides the defining surface in terms of which other features can be geographically referenced to. Therefore, this work introduces a new, more functional data representation of multi-resolution...
|
|
|
Filtering for Stochastic Evolution Equations
Kubelka, Vít ; Maslowski, Bohdan (advisor)
Filtering for Stochastic Evolution Equations Vít Kubelka Doctoral thesis Abstract Linear filtering problem for infinite-dimensional Gaussian processes is studied, the observation process being finite-dimensional. Integral equations for the filter and for covariance of the error are derived. General results are applied to linear SPDEs driven by Gauss-Volterra process observed at finitely many points of the domain and to delayed SPDEs driven by white noise. Subsequently, the continuous dependence of the filter and observation error on parameters which may be present both in the signal and the obser- vation process is proved. These results are applied to signals governed by stochastic heat equations driven by distributed or pointwise fractional noise. The observation process may be a noisy observation of the signal at given points in the domain, the position of which may depend on the parameter. 1
|
|
|
Development of HPLC method for evaluation of tryptophan metabolites
Pšenčíková, Barbora ; Kastner, Petr (advisor) ; Kučera, Radim (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Barbora Pšenčíková Supervisor: PharmDr. Petr Kastner, Ph.D. Title of thesis: Development of HPLC method for evaluation of tryptophan metabolites The essential amino acid tryptophan is a precursor of several important bioactive compounds. In recent years it was found out that tryptophan metabolites are involved in the pathogenesis of many diseases, including neurodegenerative and psychiatric disorders, autoimmune diseases, or cancer. Therefore, the analytical methods for simultaneous determination of tryptophan and its metabolites are highly required. The purpose of this diploma thesis was to develop an optimal HPLC method for determination of tryptophan and its metabolites (kynurenine, kynurenic acid, serotonin and 5-hydroxyindole-3-acetic acid) and to consider all the possible options of determination of quinolinic acid and melatonin. The chromatographic separation was carried out by Supelco Ascentis Express F5 column, 2.7 μm particle size, 15 cm × 3 mm, using spectrophotometric and fluorescence detection. Parameters of detection were for kynurenine UV detection 369 nm, 254 nm, fluorescence detection Ex/Em: 369/475; for kynurenic acid UV detection 244 nm; for...
|
|
|
Magdeburg law in Bohemia and Moravia in the period of 14th to 16th century
Dolečková, Martina ; Starý, Marek (referee)
Magdeburg law in Bohemia and Moravia in the period of 14th to 16th century Abstract This thesis aims to discuss the development and form of Magdeburg law in the period of 14th to 16th century including the analysis of existing research about Magdeburg law. The thesis is mainly focused on Litoměřice and Olomouc as the heads of Magdeburg municipal family in Bohemia and Moravia. The thesis is based on the comprehensive analysis of Magdeburg law beginning with its origins and development trends and ending with the description and characteristic of its legal sources as well as its most important legal institutes. The thesis uses not only legal-historical, but also historical and auxiliary historical methods, specifically the method of legal analysis of the text, diplomatic method, comparative method and analysis of the literature. The aim of the thesis is first, to present the state of historical research and literature on Magdeburg law. The goal here is to offer the reader a comprehensive overview of current trends in the research, pending discourse and the various approaches to Magdeburg law. The second aim is to analyse the development of Magdeburg law with the special focus on Litoměřice and Olomouc, which could be described as the most important appealing cities within the Magdeburg legal area in Bohemia...
|
|
|
Criminal Proceedings against Legal Entilities
Kajnová, Adéla ; Mulák, Jiří (referee)
This diploma thesis is focused on a comprehensive analysis of criminal proceedings against legal entities using Act No. 141/1961 Coll., On Criminal Procedure (Criminal Procedure Code) in combination with Act No. 418/2011 Coll., On Criminal Liability of Legal Entities and Proceedings against them. The adoption of Act No. 418/2011 Coll., On Criminal Liability of Legal Entities and Proceedings against them, introduced criminal liability of legal entities into the legal order of the Czech Republic since 1st January 2012 but the institute of criminal liability of legal entities is constantly evolving to respond to the needs of society. The diploma thesis is conceived from a procedural point of view with the current use of the substantive bases of the issue of criminal liability of legal entities. The thesis further explains important terms and outlines the development of criminal liability of legal entities in the Czech Republic. The aim of the thesis is to provide a comprehensive view of phases of criminal proceedings against legal entities and to evaluate selected problematic aspects of the legal regulation which are accordance with the interpretation of the professional public and with the developing jurisprudence of the domestic courts. The thesis is divided into five chapters. The first three are...
|
guest ::
RSS feed.