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Molecular biologic principles of progression of colorectal carcinoma and tehir impact on individualized oncosurgical treatment
Vyčítal, Ondřej ; Liška, Václav (advisor) ; Šimša, Jaromír (referee) ; Šiller, Jiří (referee)
Molecular biologic principles of progression of colorectal carcinoma and their impact on individualized oncosurgical treatment Background: Tumor infiltrating lymphocytes together with naturall killer have crucial role in immune reaction against colorectal carcinoma (CRCa). Executive molecule of cytotoxic immune reaction is granzym B. Natural inhibitor of granzyme B is serpine B9. Aims: Authors evaluated effect of immunohistological parameters of TIL on presence of lymph node metastasis of CRCa. Authors were also focused on selecting patients with high risk of short overall survival (OS) and short desease free interval (DFI) after radical surgical therapy. Methods: Group of 152 patients was analyzed, 63 patients in cohort classified in stage II according Union Internationale Contre le Cancer (UICC) and 89 patients in cohort UICC III (with lymph node metastasis). All of them were electively radically operated due to CRCa. Evaluating of TIL pattern was defined as peritumoral (PTL), intratumoral (ITL), intrastromal (ISL) and Crohn-like. Monoclonal antibodies against CD4, CD8, CD25, FoxP3, CD57, and granzyme B were used for immunohistological staining of TIL. Cancer cells were stained with CD56 and serpine B9 monoclonal antibody. Also endovascular (VE), lymphatic and perineural infiltration were evaluated....
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