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National Repository of Grey Literature

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Separation and characterization of GnRHs by capillary electrophoresis in classical and isoelectric buffers Šolínová, Veronika ; Poitevin, M. ; Barth, Tomislav ; Peltre, G. ; Kašička, Václav Capillary zone electrophoresis (CZE) in classical buffer-based background electrolytes and carrier ampholytes-based capillary electrophoresis (CABCE) using isoelectric BGEs (narrow pH fractions of carrier ampholytes used in isoelectric focusing) have been applied to separation and characterization of synthetic human gonadotropin-releasing hormone (hGnRH) and its fragments. From the measured effective mobilities of these peptides within a broad pH range (3.0-10.0), their isoelectric points (pI) were determined. Detailed record

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