|
|
Automatic improvements of images from 1D gel electrophoresis
Kovář, Martin ; Vítek, Martin (referee) ; Škutková, Helena (advisor)
In this bachelor’s thesis are explained the basic principles of electrophoresis and its modalities with focusing on 1D gel electrophoresis. It describes analysis of an electrophoreogram and causes of its possible distortion, and states specifications of applications of the method in microbiology, genomics and proteomics. The practical part presents development, optimization and outputs of a programme for automatic electrophoreogram analysis, which was created in Matlab environment. The analysis contains lane and band detection and computation of samples’ molecular weight. The ending of the thesis is constituted by evaluating efficiency of detection and accuracy of weight computation.
|
|
| |
|
|
Probability-based model for network traffic
Bednár, Peter ; Jelínek, Mojmír (referee) ; Molnár, Karol (advisor)
This work identifies several basic types of traffic in IP networks and describes main features. The basic network services such as HTTP, VoIP and electronic mail are discussed focusing mainly on HTTP and VoIP probability-based models for network traffic which are applied to the software simulation of real network.
|
|
|
Detection of iron-induced proteins and their effect on anthracycline production in submerged culture of Streptomyces coeruleorubidus
MICHALCOVÁ, Zuzana
In this bachelor's thesis, proteins from the group of enzymes - esterases and their possible induction after the addition of iron to the culture medium were analyzed. Furthermore, the effect of the addition of Fe2+ cations on daunomycin production under submersion cultivation conditions of Streptomyces coeruleorubidus culture was evaluated. The work is divided into two parts. The first part introduced the genus actinobacteria called Streptomyces, their occurrence, properties and possible benefits due to the production of secondary metabolites. In the second, experimental part, the use of the Streptomyces coeruleorubidus strain was described. The effect of iron addition was evaluated by HPLC analysis and native polyacrylamide electrophoresis (native PAGE). HPLC analysis showed that the addition of iron in the form of FeSO4 to the medium increased daunomycin production by approximately 21.5-fold. During native PAGE and after staining, proteins, i.e. esterases, most likely from the groups of and esterases, were detected. Those were more or to a greater extent produced by the Streptomyces coeruleorubidus if the medium contained iron.
|
|
|
Automatic improvements of images from 1D gel electrophoresis
Kovář, Martin ; Vítek, Martin (referee) ; Škutková, Helena (advisor)
In this bachelor’s thesis are explained the basic principles of electrophoresis and its modalities with focusing on 1D gel electrophoresis. It describes analysis of an electrophoreogram and causes of its possible distortion, and states specifications of applications of the method in microbiology, genomics and proteomics. The practical part presents development, optimization and outputs of a programme for automatic electrophoreogram analysis, which was created in Matlab environment. The analysis contains lane and band detection and computation of samples’ molecular weight. The ending of the thesis is constituted by evaluating efficiency of detection and accuracy of weight computation.
|
|
| |
|
| |
|
|
Probability-based model for network traffic
Bednár, Peter ; Jelínek, Mojmír (referee) ; Molnár, Karol (advisor)
This work identifies several basic types of traffic in IP networks and describes main features. The basic network services such as HTTP, VoIP and electronic mail are discussed focusing mainly on HTTP and VoIP probability-based models for network traffic which are applied to the software simulation of real network.
|
|
|
The elite of the Communist Party of Czechoslovakia on the pages of Rudé právo in 50s´ of 20th century .
STEHLÍK, Petr
This diploma thesis deals with a detailed analysis of the daily Rudé právo in 1951 - 1960. It is focused in detail on leading representatives of the Communist Party and their impact on the situation in the former Czechoslovakia in ´50s, as well as on significant events in this period. The work will consist in comparing the real facts and distorted facts about personalities and events of that time as they were presented by Rudé právo as the propaganda newspaper of the Communist Party. The main aim of the thesis is to make a reader aware of the impartiality of Rudé právo which played a dominant role in the former media scene and greatly influenced the general public who did not have the opportunity to compare information with other news. It is important as well to the reader understand how powerful the media had already been that time, when Rudé právo was able to significantly support a cult of personality mainly around Klement Gottwald and J. V. Stalin, when a reader had no opportunity to gain any information than what the governance of communist Czechoslovakia wanted to. Besides the already mentioned cults of personalities the work will deal with propaganda of communism, the Soviet Union and the anti-Americanism which was propagated mainly between 1950 - 1953 in almost every issue of the newspaper. After reading this thesis, the reader should understand the content of Rudé právo and its importance. He should realize that the main aim was to arouse belief of the citizens of Czechoslovakia that "Western world", especially the United States, is an enemy that wants to control the whole world and that its leaders are the aggressors and the enemies of peace. The Soviet Union and its leaders should then be the protection against so-called warmongers from the USA. Due to then lack of information it was very difficult for an average working Czechoslovak reader to know that the reality was quite the opposite.
|
|
|
Mikrosatelity a jejich využití v genetických analýzách
Wijacki, Jan
Microsatellites are short repetitive DNA sequences localized in both procaryotic and eucaryotic genome. It belongs to the satellite DNA located in the coding and noncoding regions of genetic information. They consist of 1-6 base pairs and the number of repetitions of individual motifs can move up in the hundreds. Microsatellites are tested using the polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE) or by capillary electrophoresis and fragment analysis. They are currently widely used as molecular genetic markers used, e.g. for paternity analysis, genetic mapping studies and genetic population and diversity studies.
|
guest ::
