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The effect of microfluidic sperm separation on the success of IVF therapy
NOVÁKOVÁ, Pavla
More and more couples are coming to assisted reproduction centres to solve conception problems. Despite initial efforts to improve lifestyle and reduce daily stress, problems in conceiving and delivering a healthy baby can be caused by one or a combination of multiple factors, and in such cases infertile couples opt for assisted reproductive treatment. In earlier times, the focus in the field of reproductive medicine was mainly on female fertility. In recent years, assisted reproductive medicine has largely focused on the field of andrology. In this regard, the development of enhanced spermiogram methods and sperm processing techniques has been developed. The current trend in reproductive embryology and andrology methods is to mimic the in vivo environment as much as possible. The aim of this bachelor's thesis was to determine whether the method of microfluidic sperm separation has an effect on sperm quality and whether the use of sperm obtained in this way influences the success of IVF treatment. In order to achieve the objectives of this study, data from 129 couples who underwent treatment in our IVF centre by donated oocytes and at the same time sperm from fresh ejaculate were used. The sperm samples were divided into two groups, namely for processing by gradient centrifugation and separation by microfluidic chip. The sperm were then used for in vitro fertilization using intracytoplasmic sperm injection method. Cycles with donated oocytes were selected to minimize the influence of female factor. The results of this work indicate that sperm processing by microfluidic chip method has a positive impact on the success of IVF treatment.
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