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Study of natural substances of ginger
Dobiáš, Vojtěch ; Vespalcová, Milena (referee) ; Veselá, Mária (advisor)
The aim of this study was to determine the quantity of natural substances in ginger. Extracts from organic ginger tea and ginger rhizome were prepared using three different solvents. Antioxidant activity and the content of polyphenols and flavonoids were determined spectrophotometrically. Effects on bacterial growth of the extracts were studied against bacteria Bacillus cereus and Serratia marcescens by agar well diffusion assay. The obtained data showed, that there is a significant amount of difference between the contents of studied compounds in the two extract samples. The highest amount of polyphenols was found in ginger tea with concentration of 0,481 mgml-1, compared to 0,078 mgml-1 found in the ginger rhizome. The tea showed a greater antioxidant activity than the rhizome. Bacterial growth was slightly inhibited only by water extracts of the tea and this was only observed against Bacillus cereus.
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Study of antimicrobial substances of ginger
Ciburová, Alena ; Skoumalová, Petra (referee) ; Veselá, Mária (advisor)
For thousands of years ginger rhizomes are used in traditional Chinese medicine. Because of his significant pungent flavour is commonly used in foods and beverages all over the world. Nowadays many of the beneficial pharmacological positive effects of ginger rhizomes are identified. Their occurrence is due to the high concentration of bioactive compounds. This thesis is focused on analysing of occurrence of bioactive compounds such as polyphenols and flavonoids and their influence on antioxidation and antimicrobial properties of samples of fresh and dried ginger. The results show that for extraction of bioactive compounds in different ginger samples is more suitable to use different types of extraction solvents. The highest antioxidation activity was measured for macerate of 100% bio ginger tea from Sonnentor company in 96% ethanol. Antimicrobial properties were showed only for 100% bio ginger tea against gram-positive bacteria Microccocus luteus.
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Use of some exotic natural extracts in cosmetics
Phan, Huong Tra ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The objective of this bachelor thesis was a general characterization of the antioxidant effect of exotic plants and their extracts. The selected plants were Zingiber officinale, Turmeric longa and Momordica charantia. Theoretical part focuses on antioxidative properties, genereal characteristics of selected plants and description of the methods applied in the experimental part. In the experimental part, content of the antioxidant activity, polyphenols, flavonoids were characterized by spectrophotometry. Major active ingredient (curcumin, gingerol) and other substances like tocopherol were determined by HPLC/UV-VIS. Finally, potential practical use of these active substances in the field of cosmetics including skin hydration measurement was tested.
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Study of natural substances of ginger
Dobiáš, Vojtěch ; Vespalcová, Milena (referee) ; Veselá, Mária (advisor)
The aim of this study was to determine the quantity of natural substances in ginger. Extracts from organic ginger tea and ginger rhizome were prepared using three different solvents. Antioxidant activity and the content of polyphenols and flavonoids were determined spectrophotometrically. Effects on bacterial growth of the extracts were studied against bacteria Bacillus cereus and Serratia marcescens by agar well diffusion assay. The obtained data showed, that there is a significant amount of difference between the contents of studied compounds in the two extract samples. The highest amount of polyphenols was found in ginger tea with concentration of 0,481 mgml-1, compared to 0,078 mgml-1 found in the ginger rhizome. The tea showed a greater antioxidant activity than the rhizome. Bacterial growth was slightly inhibited only by water extracts of the tea and this was only observed against Bacillus cereus.
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Study of antimicrobial substances of ginger
Ciburová, Alena ; Skoumalová, Petra (referee) ; Veselá, Mária (advisor)
For thousands of years ginger rhizomes are used in traditional Chinese medicine. Because of his significant pungent flavour is commonly used in foods and beverages all over the world. Nowadays many of the beneficial pharmacological positive effects of ginger rhizomes are identified. Their occurrence is due to the high concentration of bioactive compounds. This thesis is focused on analysing of occurrence of bioactive compounds such as polyphenols and flavonoids and their influence on antioxidation and antimicrobial properties of samples of fresh and dried ginger. The results show that for extraction of bioactive compounds in different ginger samples is more suitable to use different types of extraction solvents. The highest antioxidation activity was measured for macerate of 100% bio ginger tea from Sonnentor company in 96% ethanol. Antimicrobial properties were showed only for 100% bio ginger tea against gram-positive bacteria Microccocus luteus.
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Use of some exotic natural extracts in cosmetics
Phan, Huong Tra ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The objective of this bachelor thesis was a general characterization of the antioxidant effect of exotic plants and their extracts. The selected plants were Zingiber officinale, Turmeric longa and Momordica charantia. Theoretical part focuses on antioxidative properties, genereal characteristics of selected plants and description of the methods applied in the experimental part. In the experimental part, content of the antioxidant activity, polyphenols, flavonoids were characterized by spectrophotometry. Major active ingredient (curcumin, gingerol) and other substances like tocopherol were determined by HPLC/UV-VIS. Finally, potential practical use of these active substances in the field of cosmetics including skin hydration measurement was tested.
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