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Slovak National Uprising
Orság, Michal ; Terén, Laco (referee) ; Ambrůz, Jan (advisor)
The aim of the thesis was to bring events after 1945, when there was a period of remembrance in the company , which served as a therapy , politics and aesthetics . Work on the memories gradually morphed into , among other things , to the monuments that act against collective oblivion . Arise place of remembrance , which relate to historical events and private stories . Partisan monuments fill in the country which they have to remind essentially absent . Again remembering you and gets to the surface again forgotten information that creating a logical connection form the structure . This structure is subject to change , which helped by rewriting and overlapping information in different layers of the structure . Neither art does not avoid the remembrance and forgetting , and creates a theme of a strategy , which is included rewriting . Media and individual override blank sheets in the past, currently , collecting and composing the mosaic , which allows you to create your own identity.
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Polymorphisms of nutrigenetics-active genes in Vietnamese and Czech population
Pham, Khanh Ha ; Hubáček, Jaroslav (advisor) ; Freiberger, Tomáš (referee)
This Master's thesis analyzes and compares the allele distribution of some polymorphisms within nutrigenetics-active genes in the Czech and Vietnamese population. The theoretic part of this thesis describes the genes along with their polymorphisms that can contribute to the metabolism of some nutrients or which are long known for their association with the onset and manifestation of some metabolic diseases and other pathologic processes. It also discusses the possible causes of positive selection and its impact on the allele and genotype frequenccy of said polymorphisms. The own research is composed of the processes of DNA obtaining and extraction, genetic analysis' methods including PCR-RFLP and Realtime PCR along with the food frequency questionnaire (FFQ) method. Results of the analyses and questionnaire are statistically evaluated and presented with the aid of tables and graphs. The discussion brings some possible causes for said outcomes. The results show that the distribution of the risk alleles of observed polymorphisms are usually lower in the Vietnamese community than it is in the Czech population especially within: the FTO gene which can predict the development of obesity, the CTF7L2 gene which is associated with type 2 diabetes, in ADH1B which can regulate alcohol consumption and lastly...
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The impact of chimerism in DNA-based forensic analysis
Kalousková, Martina ; Šimková, Halina (advisor) ; Vaněk, Daniel (referee)
The presence of two or more zygotically distinct lineages in a single person refers to chimerism. All types of chimerism have positive as well as negative impact on health of human chimeras. Both congenital and acquired chimerism must be taken into account in diagnostics, genetics but also in the forensic DNA analysis. This phenomenon has impact on the results of individual identification and kinship determination. With the forensic analysis it is possible to detect chimerism of people under investigation. Even though these situations are rare, some cases of wrong identification proving the consequences of the chimerism are recorded.
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Genomická selekce v chovu skotu
BARTŮŇKOVÁ, Lenka
This thesis analyses genomic selection with an emphasis on cattle breeding. Genomic selection is starting to become a common element of breeding programmes of livestock animals rearing and this is a new approach to marker-assisted selection (MAS), where instead of identifying individual interconnected loci all information for predictions is obtained from markers, thus making them more accurate. This thesis aims to approach genomic selection, individual methods of selection and their uses, as much as their implementation in cattle rearing. The work devotes a section to applications of genomic selection in Czech Republic. The possible future development of genomic selection is being hinted in the conclusion.
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FINE-SCALE RECOMBINATION MAPS OF THE CATTLE GENOME INFERRED BY LINKAGE DISEQUILIBRIUM
MOHAMMADI, Fardokhtsadat
Recombination is a genetic event that occurs during meiosis and leads to the exchange of genetic material between paternal and maternal homologous chromosomes. The intensity of recombination is shown to vary across genomes between and within species, yet the determinants of recombination patterns among populations of the same species are not fully understood. In this thesis, we estimated fine-scale, breed-specific recombination maps of a subset of chromosome 25 of Braunvieh and Fleckvieh cattle for different populations with respect to inbreeding coefficients using the R package LDJump under two assumptions, neutrality and demography. Moreover, we studied the association between recombination rates and genomic features such as SNP count, GC content, and the density and nature of genes. We observed a statistically-significant, weak negative correlation between recombination rates and SNP count, where low recombination rates are accompanied by higher SNP count, and vice versa. More complex demographic scenarios as well as the level of inbreeding should be incorporated in further research using LDJump to address this possible association between SNP count and recombination rates. On the contrary, we observed no such relationship between recombination rates and GC content. We detected a substantial difference in gene density between the lowest and highest SNP-count regions of chromosome 25.
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Interaction of selected anthocyanidins with farnesoid X receptor
Jeřábková, Jana ; Pávek, Petr (advisor) ; Martin, Jan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Jana Jeřábková Supervisor: Doc. PharmDr. Petr Pávek, Ph.D. Title of diploma thesis: Interaction of selected anthocyanidins with farnesoid X receptor Human farnesoid X receptor (FXR) is a member of nuclear receptor superfamily that act as ligand-activated transcription factors. FXR binds to specific regulatory DNA regions and induces expression of many target genes. These regulated genes are involved in bile acid metabolism and transport, maintaining blood lipids, liporoteins and glucose homeostasis and also contribute to maintain intestinal bacterial balance, hepatoprotection and liver regeneration. The interest of recent studies is to test the range of FXR ligands for treatment and prevention of many diseases such as cholestais, cholesterol gallstone disease, steato-hepatitis, dyslipidemia, atherosclerosis, type 2 diabetes mellitus, metabolic syndrome, liver cancer and other forms of cancer such as breast cancer. In this experimental diploma thesis we are focused on testing of potencial ligands of human farnesoid X receptor from the group of natural plant pigments anthocyanidins (cyanidin, delphinidin, malvidin, pelargonidin, peonidin and petunidin) using the human hepatoma cell line...
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Genetic variability in sporadic colorectal cancer: Searching for novel risk, prognostic and predictive biomarkers.
Jirásková, Kateřina ; Vodička, Pavel (advisor) ; Machoň, Ondřej (referee) ; Eckschlager, Tomáš (referee)
Colorectal cancer (CRC) is a major public health problem worldwide. Despite improvements in the diagnostic process and advancement in the treatment methods, the prognosis remains poor. To improve survival rates, it is important to identify people with the predisposition for CRC and to detect the potentially curable early stage of the disease. Furthermore, identifying those who would have an adverse clinical outcome associated with a particular chemotherapy would help to avoid redundant chemotherapy burden in patients and contribute to enhanced therapeutic efficacy, while minimizing treatment-related toxicity. The aim of the Thesis was to search for novel promising diagnostic, prognostic and predictive DNA-based biomarkers of sporadic form of CRC. As each patient is genetically unique, these biomarkers would aid clinicians in better diagnosis and/or in the selection of an optimal type of therapy for an individual CRC patient based on their molecular profile. In order to explore this issue, we investigated several candidate genes in healthy individuals as well as in newly diagnosed cancer patients. The major outcomes of this PhD study, which were fully reported in seven publications included in the present Thesis, are 1) The observation of several candidate single nucleotide polymorphisms in microRNA...
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Ecophysiological relevance of extreme sensitivity of orchids to nitrates
Figura, Tomáš ; Ponert, Jan (advisor) ; Tylová, Edita (referee)
Many orchid species are seriously endangered at present. Reasons for their disappearing from natural habitats remain often unclear. Orchids depend on mycorrhizal symbiosis in nature, however only little is known about this symbiosis. Seeds of some species do not germinate in vitro, making their cultivation for scientific and rescue purposes impossible. We found that seed germination of one of such reluctant species, Pseudorchis albida, is strongly inhibited by nitrates even at extremely low concentrations. As this species prefers oligotrophic mountain meadows, nitrate-induced inhibition probably take place in natural conditions. Surprisingly, we found similar but slightly weaker inhibitory effect also in typical mesophillic species and even in slightly eutrophic ones. The sensitivity to nitrates correlates with trophy level of species canopy. This inhibitory effect of nitrates could be weakened by application of a range of growth regulators, including auxins, cytokinins and gibberellins, and also by mycorrhizal fungi. The action of nitratereductase is essential for this inhibitory effect of nitrates. Experiments with NO donors, scavengers, and NO quantification are pointing right at NO as compound which perhaps mediates nitrate inhibitory effect. Based on these results, the nitrate inhibitory...
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