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Study of diffusion properties of vesicular systems using Fluorescence Correlation Spectroscopy
Rašticová, Barbora ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This master's thesis deals with the study of the diffusion properties of vesicular systems composed of two oppositely charged tensides SDS and CTAB with the addition of the double-chain tenside DODAC and cholesterol. The aim of this work was to select suitable fluorescent probes and investigate their use in imaging these systems using dynamic light scattering and fluorescence correlation spectroscopy. Different concentrations of hyaluronan were also added to the prepared systems. Two representatives of carbocyanine (DiI, DiO) and two xanthene (F16, RBOE) dyes were selected as fluorescent probes. The suitability of their use was verified for all probes except RBOE. The measurement results from the two methods were also compared. It was found that the data do not correlate with each other, due to the considerable complexity of the analyzed systems and the available mathematical models to fit the FCS curves are therefore insufficient.

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