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Microscopic fungi in caves and other underground shelters
Horáková, Karolína ; Kubátová, Alena (advisor) ; Hujslová, Martina (referee)
This Bachelor thesis is meant as a literature search concerned with diversity of microscopic fungi in caves, prospectively in underground spaces which were made by humankind. The caves represent unique environment for living organisms which is characteristic with specific microclimatic conditions and with deficient sources of nutrients. I have concentrated my focus to micromycetes which were isolated from sediments, atmosphere, dead insect and excrements in caves. The next part of my Bachelor thesis is aimed at species Geomyces destructans which causes so called white-nose syndrome illness - geomycosis on hibernating bats. It is an actual problem which interests scientists such as mycologists, zoologists and parasitologists.
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Monitoring the transfer of albendazole from the sheep faeces to fodder by LC-MS
Sochová, Andrea ; Skálová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Andrea Sochová Supervisor: prof. RNDr. Lenka Skálová, Ph. D. Title of diploma thesis: Monitoring of albendazole transfer from ovine faeces to fodder plants by LC-MS In general, drugs might be a significant source of pollution in the environment especially drugs with high persistence. Albendazole (ABZ) belongs to a benzimidazole group of anthelmintic drugs. These drugs are regularly and frequently used to limit and treat parasitic infections in animals. ABZ enters the environment via animal excrements and it can have negative effects on non-target organisms. Laboratory experiments have shown that the plants can uptake and even biotransform the ABZ, however, it is not known whether these phenomena occur in the real field conditions. The present study monitors the transfer of ABZ and its transformation products (TPs) from the faeces of treated sheep to common fodder plants such as Medicago sativa and Trifolium pratense. We wanted to know whether the possibility of transferring these compounds from excrement into the soil and from soil to plants exits in real field conditions. Our study successfully revealed the occurrence of ABZ TPs (ABZ-SO and ABZ-SO2) in both fodder plants. The highest...
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Sexual dimorphism of rat gut microbiota composition and intestinal immunity
Kalousová, Pavla ; Kovařík, Miroslav (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Pavla Kalousová Supervisor: PharmDr. Miroslav Kovařík, Ph.D. Title: Sexual dimorphism of rat gut microbiota composition and intestinal immunity Background and Aim: Many factors can influence the composition of gut microbiota and the immune system. It is well-known that one of those factors is sex. This sexual dimorphism can lead to a specifically adjusted treatment of diseases for different gender and nutritional interventions. This study focuses on analysing sexual differences in gut microbiota and intestinal immunity in adult rats. Methods: Caecal content from 12-week-old female and male Wistar rats were collected and analysed by DNA-sequencing technique to characterize microbiota composition. ELISA test was performed to quantify the concentration of IgA in faeces and gut wash. Flow cytometry determined the concentration of IgA-coating bacteria in faecal samples. Results: Metagenomic analysis revealed that female gender presents 1 phylum, 4 families, 13 genera, and 13 species which are not present in male rats. Only one male-specific colonization was observed at the species level. Quantitative analysis showed a higher proportion of Firmicutes phylum in males which was associated with...
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Microbial association with house dust mites
Molva, Vít ; Hubert, Jan (advisor) ; Žůrek, Luděk (referee)
The house dust mites (HDM; Dermatophagoides farinae and Dermatophagoides pteronyssinus) obtain nutrients from the debris of the skin, hairs and nails, that get off the human and animal body. These debris are covered by microorganisms and the microorganisms are the key factor in the survival of HDM in human-made environments. In this study we made manipulative experiments with the extract from spent growth medium (SPGM). SPGM is the medium after mite cultivation, composed from mite feces, debris of the diet, dead mite bodies and microorganisms. The extract from SPGM (one and three-month-old mite cultures) was used as the source of microorganism to transfer them into diet of D. farinae a D. pteronyssinus. The composition of the bacterial and fungal microbiomes differed between the HDM species, but the SPGM extract addition into diet influenced only the bacterial profile of D. farinae. In the D. farinae microbiome of specimens on SPGM-treated diets compared to those of the control situation, the Lactobacillus spp. profile decreased, while the Cardinium, Staphylococcus, Acinetobacter, and Sphingomonas profiles increased. The addition of SPGM extract decreased the microbial respiration in the microcosms with and without mites in almost all cases. Adding SPGM did not influence the population growth of D. farinae,...
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Sexual dimorphism of rat gut microbiota composition and intestinal immunity
Kalousová, Pavla ; Kovařík, Miroslav (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Pavla Kalousová Supervisor: PharmDr. Miroslav Kovařík, Ph.D. Title: Sexual dimorphism of rat gut microbiota composition and intestinal immunity Background and Aim: Many factors can influence the composition of gut microbiota and the immune system. It is well-known that one of those factors is sex. This sexual dimorphism can lead to a specifically adjusted treatment of diseases for different gender and nutritional interventions. This study focuses on analysing sexual differences in gut microbiota and intestinal immunity in adult rats. Methods: Caecal content from 12-week-old female and male Wistar rats were collected and analysed by DNA-sequencing technique to characterize microbiota composition. ELISA test was performed to quantify the concentration of IgA in faeces and gut wash. Flow cytometry determined the concentration of IgA-coating bacteria in faecal samples. Results: Metagenomic analysis revealed that female gender presents 1 phylum, 4 families, 13 genera, and 13 species which are not present in male rats. Only one male-specific colonization was observed at the species level. Quantitative analysis showed a higher proportion of Firmicutes phylum in males which was associated with...
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Endoparazitózy skotu ve vybraných chovech Moravskoslezského kraje
Ručková, Amálie
Raising cattle is one of the key factors in animal production. The main products are milk, meat and manure. It is very important to ensure good conditions for breeding and veterinary prevention to avoid economic losses and keep the profitability. The main goal of the thesis was to compare appearance of endoparasites in cattle on two selected farms in Moravian-Silesian region, in respect of breeding technique and technology used on this farms. Between the years 2014-15 samples of feaces was collected in random intervals on both farms from combined and milk breeds. There was collected 300 samples in total from both farms. On the first farm group of cattle, which consisted of holstein milk breed, was raised without the access to pasture. On the second farm the group of cattle B,which consisted of Fleckvieh Breed, had access to pasture whole year except the winter months. The samples were collected into plastic containers mostly in fresh state. The samples were analyzed by non-invasive coprological flotation method by Brezy. Each findings of parasites were classified into classes and the intensity of infection and prevalence of parasites was determined in individual farms. In the herd (A) were diagnosed coccodias with a prevalence of 6 %, 3 % Ostertagia and Cooperia 1%. In the herd (B) were recorded prevalence of coccodias 9 %, Trichostronglus 13 %, Ostertagia 33 %, Cooperia 1 %, Oesophagostomum 3%, Trichuris 4 % and Capillaria with prevalence of 2 %. In any of these herd no flukes and tapeworms were found. Overall the prevalence and intensity of infection of cattle endoparasites were very low during the research.
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Pollution produced carp general, depending on the fullness of the digestive tract.
HAVLÍNOVÁ, Šárka
The aim of my bachelor thesis was to determine and compare the differences in the levels of pollution produced by fish through their metabolic activities depending on the fullness of their digestive tract. The pollution production was detected in 4 groups of fry of the common carp: 1) the fish had no access to food for 2 days before the beginning of the experiment; 2) the fish did not eat for 10 days before the beginning of the experiment; 3) the fish had a limited food intake (pellets KP1) before the beginning of the experiment; 4) the fish accepted spontaneously feed (2% by weight of the stock). The experiments were carried out in laboratory conditions. In the beginning of the experiment, the fish of individual groups and known weight were placed in experimental aquariums filled with 20 l of tap water. The experiments took 24 hours and after that the fish were moved back into its origin tank. Water from the experimental aquariums was filtered through a screen with 40 micrometr mesh size. Both, filtered water ("aqueous phase excrements") and solids ("solid phase excrements") were analysed. Solids were analysed for a content of dry matter, total phosphorus (P) and nitrogen (N). Filtered water was analysed for a concentration of suspended solids, total nitrogen, total ammonia, total phosphorus, chemical oxygen demand (CODMn, and CODCr) and biochemical oxygen demand (BOD5). Aqueous phase excrements was filtered again through a glass filter (1.2 micrometr) and the filtrate was analysed for concentration of ammonia nitrogen [N (NH3 + NH4+)], nitrate nitrogen [N-NO3-] and orthophosphate phosphorus [P-PO43-]. Pollution detected in solids and in filtered water was related to 1 kg of live weight of fish per day. The parameters characterizing pollution excreted by fish achieved in the 4th group i.e. fish, which accepted spontaneously feed the highest values. The fish of this group secreted on average of 4,154 mg.kg-1.day-1 of suspended solids (particles 1.2 micrometr), 105,5 mg.kg-1.day-1 of total phosphorus and 1,342 mg.kg-1.day-1 of total nitrogen. In the aqueous phase excrements, there was detected on average of 637 mg.kg-1.day-1 of ammonia nitrogen 4.9 mg.kg-1.day-1 of phosphate phosphorus.4,561 mg.kg-1.day-1 of CODCr, 2,634 mg.kg-1.day- 1 of BOD5. The values detected in the other groups were times lower.
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Microscopic fungi in caves and other underground shelters
Horáková, Karolína ; Kubátová, Alena (advisor) ; Hujslová, Martina (referee)
This Bachelor thesis is meant as a literature search concerned with diversity of microscopic fungi in caves, prospectively in underground spaces which were made by humankind. The caves represent unique environment for living organisms which is characteristic with specific microclimatic conditions and with deficient sources of nutrients. I have concentrated my focus to micromycetes which were isolated from sediments, atmosphere, dead insect and excrements in caves. The next part of my Bachelor thesis is aimed at species Geomyces destructans which causes so called white-nose syndrome illness - geomycosis on hibernating bats. It is an actual problem which interests scientists such as mycologists, zoologists and parasitologists.
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Optimization of microclimate parameters in dairy farms, while reducing the energy consumption of selected technological systems
Češpiva, Miroslav ; Pecen, Josef (advisor) ; Ján, Ján (referee)
Thesis is focused on the measurement and evaluation of microclimate parameters in barns for dairy farming and the determination of the energy consumptions of selected technologies in these barns. The aim of this work was to design and eventually to implement adjustments in order to improve the parameters of cowshed microclimate and reducing the energy consumption of selected technologies in depends on results of the measurements. Three structurally different cowsheds with different slurry clearing technologies were selected for monitoring. I focused on the measurement of temperature and relative humidity in relation to temperature- humidity index and its impact on heat stress on dairy cows. I measured airflow in the cowsheds and I determinate concentrations and emissions of ammonia and methane in the cowsheds in different seasons. I monitored light intensity in the cowsheds and I determined the dependence of building solutions on the use of outdoor light to illuminate the interior of the barns. The energy consumption of the lighting system depending on the season and the consumption of additional fans on the air temperature were studied.
On the bases of results of the experimental part I designed and implemented lighting system, controlled by light intensity in a cowshed and energy-saving ventilation in the cowshed - type K105. I suggested a system of continuously monitoring of climatic parameters in the cowshed with immediate calculation of temperature- humidity index.
Measurements have shown the dependence of gases concentrations on the way of slurry clearing technologies. The building solutions of cowsheds and type of ventilation proved a significant influence on the temperature- humidity index during the summer.
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Utilization of biomass energy
Horváth, Martin ; Bok, Jaromír (referee) ; Procházka, Zdeněk (advisor)
Restoring sources of a energy and their development brings questions how these can be these sources the best utilized. The main aim of my bachelor`s thesis is an acquainted with methods of biomass conversion to energetic potential and his advantage. Than is mentioned proper project of the biogas plant and calculation of produced electric energy quantity in select cogeneration unit base on data on specific excrements production bred pigs and biogas production from slurry. The result is a variant evaluation of economy of the biogas plant operation for agricultural enterprise according to number of bred pigs and possibility of electric energy utilization.
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