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Sledování úrovně mikrobiální kontaminace vybraných druhů čajů
Polášek, Viktor
This Diploma thesis describes the history of tea, its charakterisic, technologic processing, chemical structure and effect on human organism, as well as requirements of legislation. This is followed by characterization of microbes which contaminate teas, products of their metabolism which have negative effects on human health (especially mykotoxins) and struglge against these microbes. Experimental part is orientated on assignment of significant groups of microbes in samples of tea in draw leaves and prepered tea.
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Rizika plísní a mykotoxinů v chovu zvířat
BOHÁČKOVÁ, Lenka
The aim of this work is to provide information about what toxigenic fungi and mycotoxins are. The mycotoxins were highlighted the most important types of them, the characteristics, biological effects, methods of determining. Aflatoxins are naturally commonly occurring mycotoxins, which are produced by toxigenic filamentous fungi of the genus Aspergillus. These are substances with a very high toxicity. In conclusion, there are described the methods of detection, prevention and decontamination of mycotoxins. The decontamination is carried out by physical, chemical or biological methods.
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Mykotoxiny v obilovinách a jejich vliv na zdraví hospodářských zvířat
Černá, Izabela
The thesis deals with the impact of mycotoxins on animal organism is today very closely explored and scientific researches are still at the beginning. Until recently, it was the influence of mold and their derivatives overlooked. This work is focused on the impact of derivatives of the fungi on the body of livestock, but also domestic (domesticated) animals. The aim of this research work was to gain scientific information based on specific research and observations. With the subsequent order to continue research in particular in the follow-up master study.
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Air Conditioning equipment
Frühauf, Patrik ; Rubina, Aleš (referee) ; Rubinová, Olga (advisor)
This thesis deals with the relationship between HVAC systems and internal microclimate of buildings. The work discusses briefly about different components which are formulating internal microclimate. More details are then focused on the issue of microbial microclimate.
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Radiators and microbial microclimate
Raputa, Pavel ; Rubinová, Olga (referee) ; Počinková, Marcela (advisor)
This master thesis describes the design of heating in a new one-storey nursery school in Kuřim. The heating systém is designed as a low-temperature systém with a forced circulation of water and a horizontal piping. Three kinds of radiators are used in the design. The panel radiators, tube radiators and free-standing convectors. In this project there are two varinats of the heat source for buliding. The first variant is two gas condensing boilers and the second variant is two heat pumps of an air/water split design. The disign includes air heating in the unit of air conditioning and hot water preparation. The experimental part of the master thesis is focused on microbial microclimate in buildings and radiators contamination by microorganisms. It contains four different experiments, their realization, results and evaluation.
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Production and characteritzation of extracellular hydrolases from selected moulds
Skoumalová, Petra ; Čarnecká, Martina (referee) ; Márová, Ivana (advisor)
This diploma thesis is focused on study of potential production of extracellular hydrolytic enzymes. The theoretical part deals with characterization of selected hydrolytic enzymes, their catalytic properties, the possibility of extracellular hydrolase production by fungi and their applications. In experimental part production strains Aureobasidium pullulans, Fusarium solani and Phanerochaete chrysosporium were used. Productions of cellulase, amylase, xylanase, lipase, protease and lignin-degraded enzymes (laccase, manganese- dependent peroxidase, lignin peroxidase) were observed. Cultivations were carried out in submersed mode in mineral medium supplemented by waste co-substrates such as wheat bran, corn bran, rice bran and oat bran, sawdust, rice, apple fiber, egg pasta and egg-free pasta. Production of enzymes depended on the substrate type and time of cultivation. The highest cellulase, xylanase and amylase activities were measured in the first period of cultivation (3 to 7 day). Lignin-degraded enzymes and proteases were produced at the end of cultivation (7 to 10 days). Lipolytic activity was detected only in A. pullulans, where the activity increased with time of cultivation. The highest value was determined during cultivation on wheat bran (3.6 nmol/ml.min). The highest xylanase and celulase activity (170.3 nmol/ml.min, 248.0 nmol/ml.min) were determined during cultivation of F. solani on corn bran. The highest amylase activity (111.8 nmol/ml.min) was reported in P. chrysosporium during the cultivation on rice. The highest protease activity (68.0 nmol/ml.min) was determined in F. solani grown on wheat bran. The best producer of laccase was A. pullulans, the highest production was recorded for egg-free pasta (27.0 nmol/ml.min). The maximum lignin peroxidase activity (12.5 nmol/ml.min) was measured during the cultivation of F. solani on egg pasta, while the highest yield of Mn-dependent peroxidase (7.7 nmol/ml.min) was achieved during the cultivation of A. pullulans on wheat bran. Lignin-degraded enzymes behaved as inductive, while the other enzymes were produced in mineral medium too. Activity of cellulase in the mineral medium was in A. pullulans strain higher than in media with waste substrates. Enzymes produced into A. pullulans medium were purified by ultrafiltration, ion exchange chromatography and gel filtration.
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Factors influencing the quality of red wine
Zechmeisterová, Lucie ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
In my thesis, I focused on monitoring of microorganisms in the sample of red grape juice and on the interactions between yeasts, bacteria and filamentous fungi. Three different media were applied for the cultivation of microorganisms; firstly for monitoring of total volume of microorganisms, secondly for yeasts and third time for lactic acid bacteria. The indirect method was used for the determination of the amount of viable cells. This method consists in enumerating of visible macroscopic colonies grown up on agar plates. When the cells grew up, the forms of colonies were analyzed visually and the morphology of microorganisms was detected microscopically. The operating time of enzymes in grape juice in the production of red wine was monitored after application of commercial enzymatic preparation. The enzym action in grape juice was observed on the basis of the process of degradation of high – molecular substrate by enzymes through the use of Ubbelohd´s viscometer. The research findings provided a lot of knowledge about the occurance of microflora in the process of production of red wine. The commercial preparations added to grape juice played a significant role.
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Enzymatic hydrolysis of wastes associated with coffee production
Kovářová, Markéta ; Skoumalová, Petra (referee) ; Obruča, Stanislav (advisor)
This bachelor thesis is focused on study of potential production of extracellular hydrolytic enzymes by microorganisms – bacterium and moulds, which have been cultivated on spent coffee grounds. The theoretical part deals with characterization of coffee and utilization of coffee by-products. There are also subscribed microorganisms and enzymes which have been noticed. In experimental part coffee ground was used as the sole substrate for production of extracellular hydrolytic enzymes. Productions of protease, cellulase, mannase and lipase enzymes were observed. None-identified isolate of mould spontaneously contaminating spent coffee grounds was identified as the best producer of these enzymes. Subsequently the conditions of cultivation such as water content and shaking vs. static cultivation of this moulds were optimized. Further, we performed partial purification and pre-concentration of the enzyme cocktail by ultrafiltration, ultradialysis and PAGE-SDS characterization of extracellular enzymes was performed as well.
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