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Comparison of antimicrobial effect of emulsions for cleaning and dissinfection
Chrápavá, Edita ; Dzurická, Lucia (referee) ; Márová, Ivana (advisor)
Currently, hand disinfectants and other disinfectants are in high demand. Due to the pandemic and the spread of Covid-19, they are used by society daily. The reason for their use is not only to prevent the spread of bacteria and other microorganisms from the hands to another part of the body or objects, but also to prevent the spread of the disease. The subject of the bachelor thesis is the control of antimicrobial effects of several commonly available disinfectants using broth and agar dilution methods. From the measured data, the absorbance was processed and subsequently viability of the culture.
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Analýza hygienické úrovně produkce masa
Klíčová, Veronika
The purpose of the diploma thesis is to describe problematics of production and processing of pork and beef in terms of hygiene and microbial quality. It describes hygiene requirements in meat factories, slaughterhouses and deboning rooms, including the consequences of non-compliance. It deals with microorganisms responsible for decreasing quality of meat and pathogens causing alimentary diseases after consumption. Practical part compares microbial contamination of environment in two small and one larger deboning room including pig and cattle carcasses imported in these manufactories. Samples obtained in March, June and October show all tested deboning rooms as fully compliant in terms of purity and carcasses fulfilled microbial limits according to Commission Regulation (EC) No 2073/2005 which implies a high level of the meat quality in both cases from retailers and also from wholesalers. With proper handling of the meat after its purchase there is no alimentary risk.
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Vliv probiotik na zdravotní stav králíků
Matoušková, Anna
This bachelor thesis summarizes knowledge about probiotics and their effect in animal organism with focus on rabbits. In recent years probiotic microorganisms have been increasingly used in livestock and breeding pet animals. They are mainly used as feed additives to prevent, improve the immune system, treat diarrhea, and improve overall health. The next part of the thesis defines the concept of health, describes the physiology of the gastrointestinal tract of the rabbit and focuses on the natural microflo-ra of the internal environment of the digestive system. The main task is to focus on the influence of probiotic microorganisms on maintaining and improving the ideal health of rabbits, their action and interaction with beneficial and pathogenic microorganisms natu-rally contained in the gastrointestinal tract. At the end of the thesis there are listed forms and applications of probiotic preparations, as well as a list of some products used in rab-bit nutrition.
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Mikrobiologické parametry výroby, skladování a distribuce raw potravin
Vávra, Jaroslav
Bachelor thesis Microbiological parameters of production, storage and distribution of raw food includes theoretical and practical part. Literary research is a compilation of public and expert attitudes to raw foods, legislative regulations for used raw materials and food for immediate consumption, basics of production technology, knowledge of microbes, their metabolism products and possible health risks associated with them. The experiment was a basic microbiological analysis of raw bar samples to determine the total number of microorganisms, fungi and yeasts, coliforms, and the presence of Eschericia coli. The gained results of the experiment were subsequently compared with legislative regulations, especially with the norm ČSN 56 9609. The last part of the experiment was a short rough sensory evaluation. Although many microorganisms have been found in the products, there hasn‘t been over limit.
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Microbiological hazards in HVAC systems
Musil, Zdeněk ; Charvát, Pavel (referee) ; Kolbábek, Antonín (advisor)
This bachelor thesis deals with microbiological hazards in HVAC systems, especially in air condition systems. There is noted the basic information from related fields. The same microbiological hazards are divided in health risk and in microbiological corrosion. In the last part of this thesis are mentioned possibilities of their elimination and recommendation for prefiguring of them.
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Study of sterilization effect of diaphragm discharge in liquids
Holíková, Lenka ; Slámová, Jitka (referee) ; Kozáková, Zdenka (advisor)
The main objective of this thesis is to study the sterilizing effect of diaphragm discharge in liquids. Sterilization is a process, which can eliminate all forms of life. Generally, sterilization is divided into physical and chemical methods. Plasma sterilization is ranked among physical methods although the action of chemical processes participates in the decontamination as well. Effects of UV radiation, free radicals and temperature are utilized in these methods. Fungi spores of Aspergillus niger F8189 and bacteria spores of Bacillus subtilis are selected as model organisms. Aspergillus niger microorganism is suitable for its resistance to changes in pH and it is viable in a wide range of pH values. Bacillus subtilis is chosen because of a good heat resistance. Diaphragm discharge is one of the possible types of electrical discharges in generated liquids. It is a low-temperature plasma, which is generated using a high DC voltage. In the created plasma streamers various physical and chemical processes rise. The chemical processes are mainly the generation of active species and particles that initiate subsequent chemical reactions and attack mould spores and bacteria in liquids. The physical processes include shock waves, strong electric field and ultraviolet radiation. Experiments were conducted in a reactor with separate cathode and anode space. In the dielectric barrier PET diaphragm with a pin-hole (initial diameter of 0.4 mm) was attached. Enlargement of the hole was observed as a result of degradation of material at the edge of holes due to the discharge. Degradation of spores was observed, depending on the time and on the input power. Other parameters measured were pH, conductivity and temperature. Experiments did not show any difference of discharge activity in the cathode and anode area. The main factor in the removal of spores Aspergillus niger was probably the temperature because no demonstrable effect of sterilization by the diaphragm discharge operation had been observed when heat resistant Bacillus subtilis was used. Also, it was made an attempt, which has been studied only the temperature effect of the fungi spores of Aspergillus niger. The samples were placed in a thermoregulator, where it was simulated the same temperature increase as at the discharge. The experiment had a similar course as an attempt at the discharge.
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Thermophilic microorganisms
Sůkalová, Kateřina ; Vránová, Dana (referee) ; Babák, Libor (advisor)
This bachelor’s thesis is focused on distribution thermophilic species from among microorganisms, characteristic important genera, in which these microorganisms we can find and detailed description choice species. There is described origin of thermal environment and locations of habitation thermophilic microorganisms with intention on geothermal areas, where is found the biggest number of these species. In consequence of thermal stability of enzymes these microorganisms are used in food industry, in waste treatment, in medicine and for their production of thermostabile enzymes.
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Degradation possibilities of synthetic macromolecular materials centred on biodegradations
Zichová, Miroslava ; Ondruška, Vladimír (referee) ; Babák, Libor (advisor)
This work deals with the degradation possibilities of synthetic macromolecular materials. Mechanical, chemical and chemo-physical degradation is described, but the work is focused on biodegradation. Polymer biodegradation is its decomposition by microorganisms. The aim of the work is to show the advantages of biodegradation and to arrange the knowledge acquired about the biodegradation of particular macromolecular materials. There is resumed the information about the degradation of polyurethanes as an exception among synthetic polymers, which are mostly resistant to biodegradation. Attention is also paid to biodegradable polymers.
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Charakterizace zátěže obyvatel malých sídel škodlivinami z ovzduší a znečištění ovzduší bioaerosoly: Znečištění ovzduší bioaerosoly
Státní zdravotní ústav, Praha ; Kvasničková, Simona ; Matějů, Ladislava ; Kazmarová, Helena ; Kotlík, Bohumil
Cílem dílčího úkolu II bylo vypracovat obecně použitelnou metodu 24 hodinových odběrů vzorků ovzduší pro měření výskytu bioaerosolů, zhodnotit výskyt bioaerosolů v blízkosti vybraných zemědělských zařízení, popsat sezónní kolísání výskytu bioaerosolů a vliv meteorologických parametrů a zhodnotit míru rizika výskytu bioaerosolů. Práce zahrnovala čtyři etapy řešení. V prvé etapě řešení byla provedena rešerše dosud známých faktů o vzorkování bioaerosolů / živých částic a zvolen typ vzorkovače. V druhé etapě řešení bylo provedeno sledování denních profilů výskytu bioaerosolů / živých částic v lokalitě, která nebyla zatížena zvýšeným výskytem živých částic z jakéhokoliv zdroje znečištění jako jsou např. kompostárny, čistírny odpadních vod, zemědělská živočišná výroba apod. Ve třetí a čtvrté etapě byl zjišťován vliv zdroje znečištění jako je drůbežárna a velkochov vepřů na výskyt bioaerosolů/ živých částic v jejich okolí. Zároveň byl sledován vliv některých mikroklimatických podmínek na výskyt diskutovaných živých částic. Jako doplnění hodnocení vlivu významného zemědělského objektu bylo provedeno i hodnocení pachové zátěže.
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