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Biosensors based on modified nanoparticles
Mihajlović, Ana ; Majzlíková, Petra (referee) ; Hubálek, Jaromír (advisor)
This thesis deals with biosensors based on modified semiconductor quantum dots(AIIBIII). This work is divided into three main parts. The first deals with the theory necessary for the use of QDs in bioapplications, there are described methods of synthesis, application and bioconjugation of QDs . In the practical part, GSH-CdTe, CdTe-MPA and TGA-CdTe quantum dots were prepared by direct colloidal synthesis. These quantum dots were then modified by EDC in order to increase affinity to BSA (bovine serumalbumin). For the analysis of samples were used Infinite M200,Tecan and HORIBA Jobin Yvon FluoroLog detector Quanta . The third part is the summary of results and work achievements.
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Synthesis of spherical gold nanoparticles for biomedical applications
Gablech, Evelína ; Adam, Vojtěch (referee) ; Drbohlavová, Jana (advisor)
Tato práce se zabývá syntézou sférických zlatých nanočástic pro biomedicínské aplikace. Zlaté nanočástice byly syntetizovány ekologicky nezávadnými metodami, kterým je věnována i značná část rešerše. Cílem bylo nasyntetizovat stabilní koloidní zlaté nanočástice vhodné pro různé biomedicínké aplikace zejména, pro in vivo a in vitro zobrazovací metody, kterých přehled je take obsažen v teoretické části práce. Také byl proveden test cytotoxicity, jelikož částice mají být použitelné pro in vivo aplikace. Částice byly dale charakterizovány metodami SEM, DLS a UV-VIS.
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Solution of Continuous Systems by Evolutionary Computational Techniques
Lang, Stanislav ; Šeda, Miloš (referee) ; Olehla, Miroslav (referee) ; Matoušek, Radomil (advisor)
The thesis deals the issue of solution of continuous systems by evolutionary computational techniques. Evolutionary computing techniques fall into the field of softcomputing, an advanced metaheuristics optimization that is becoming more and more a method of solving complicated optimization problems with the gradual increase in computing performance of computers. The solution of continuous systems, or the synthesis of continuous control circuits, is one of the areas where these advanced algorithms find their application. When dealing with continuous systems we will focus on regulatory issues. Evolutionary computing can then become a tool not only for optimization of controller parameters but also to design its structure. Various algorithms (genetic algorithm, differential evolution, etc.) can be used to optimize the parameters of the controller, for the design of the controller structurewe usually encounter so called grammatical evolution. However, the use of grammatical evolution is not necessary if appropriate coding is used, as suggested in the presented thesis. The thesis presents a method of designing the structure and parameters of a general linear controller using the genetic algorithm. A general linear regulator is known also as so called polynomial controller, if we encounter the polynomial theory of control. The method of encoding the description of the general linear controller into the genetic chain is crucial, it determines a set of algorithms that are usable for optimization and influence the efficiency of the calculations. Described coding, effective EVT implementation, including multi-criteria optimization, is a key benefit of this work.
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Advanced ceramics for tissue engineering
Vojtíšek, Jan ; Koplík, Jan (referee) ; Bartoníčková, Eva (advisor)
This bachelor´s thesis deals issues of ceramics materials suitable for tissue engineering. An artificial substitute for human tissue can be divided to several generations. This include inert, bioactive and bioresobable materials. Resorbable substitutions are prepared from materials, which could substitute host tissue, for example hydroxyapatite. HA can be prepared in different ways (dry and wet way). To better application in medicine, ceramics foams prepared by in situ foaming or replication technique are using. Experimental part of this paper deals preparing of hydroxyapatite powder and process optimization. Samples was characterized by IR and Raman spectrometry, XRD analysis and particle size was studied by SEM. Ceramics foams was prepared by in situ foaming from Al2O3, HA and composites (in ratio 50 – 50, 75 – 25, 90 – 10). Porosity was evaluated by stereomicroscope, density and level of sintering was studied by SEM microscopy.
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Effect of environment on the photocatalytic properties of copper oxides
Šmatlo, Filip ; Vaněk, Jiří (referee) ; Vanýsek, Petr (advisor)
V této práci byl popsán princip fotokatalýzy. Práce se soustřeďuje na kovové oxidy, které mají široké využití ve fotokatalytických aplikacích. Bylo také popsáno různé použití fotokatalytických materiálů. Tato práce se zaměřuje zejména na využití fotokatalytických materiálů pro rozklad organických vodu znečisťujících látek. Popsaný fotokatalyzátor je oxid měďný, který má velmi dobré vlastnosti pro rozklad organických látek pomocí fotokatalýzy. Schopnost rozkladu organických látek oxidu měďného byla popsána na rozkladu organického barviva methylová oranž.
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Functionalization of gold nanoparticles for imaging
Jakubechová, Jana ; Pekárková, Jana (referee) ; Drbohlavová, Jana (advisor)
The diploma thesis deals with synthesis of gold nanoparticles and their surface functionalization suitable for in vitro imaging. In this view there are requirements for optical properties such as stability, monodispersity and no presence of cytotocxicity. In order to fulfill these demands the synthesis by Turkevich method with surface modification by glutathione and polytethylenglycol was performed. Analytical methods such as DLS, SEM and Zeta potential measurement were utilized to characterize the physical and chemical properties of synthesized gold nanoparticles. Finally, MTT assay was performed to evaluate toxicity of gold nanoparticles using HEK 293 cell line.
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Network Configuration Synthesis and Verification
Černeková, Alžbeta ; Veselý, Vladimír (referee) ; Ryšavý, Ondřej (advisor)
The subject of this master's thesis is to address the topic of network devices configuring. It resolves the problems related to simplifying certain parts of configuration while eliminating frequent errors or issues. It introduces a solution to setting of IP addresses on router's interfaces. Furthermore it presents the generation of configuration for particular dynamic routing scenarios and its application and methods for its verification on network devices. It also demonstrates the preparation of configuration of VPN tunnel between two devices. The conclusion of this thesis contains possibilities for further extensions or improvements.
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Preparation and characterization of poly-3-hydroxybutyrate copolymers
Kolomazník, Vít ; Kupka, Vojtěch (referee) ; Porubský, Tomáš (advisor)
This thesis evaluates the synthesis of copolymers based on poly-3-hydroxybutyrate (PHB). The goal is to create a material with the highest possible concetration of PHB with the smallest possible volume of crystal phase. PHB with lower molecular weight and hydroxyl end groups used for polyurethane synthesis was prepared by alcoholysis of PHB polymer with 1,4 butanediol. Polyethylene glycole (PEG) of various molar mass and ratio values was chosen in this thesis as a second reacting substance. Another substance used for copolymeration with PHB was the poly(butylene adipate-co-terephthalate) PBAT. Both variants used hexamethylenediisocyanate (HDI) as a non-toxic bonding agent. From these substances, a block copolymers comprised of soft and hard segments were formed. Hard segments are a result of PHB and it‘s crystalic structure. Soft segmets are comprised of PEG or PBAT macromolecules. The influence of soft segments of various length and molar ratio of reactants on overal polyurethane properties was evaluated. Prepared materials were charactrerized by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and infrared spectroscopy (FTIR). Molecular weight was measured by gel permeation chromatography (GPC).
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Synthesis of core/shell quantum dots for diagnostics
Mihajlović, Ana ; Vaculovičová, Markéta (referee) ; Pekárková, Jana (advisor)
This thesis deals with biosensors based on modified semiconductor core/shell quantum dots (QDs) for diagnosis. The work is divided into four main parts. The first one discusses the theory required for the use of QDs in bioaplications, there are described methods of synthesis, modification, application and bioconjugation of QDs. In the experimental part, CdTe/ZnS QDs with core/shell structure were prepared, in which the core was modified by MPA, GSH and TGA. In the next step, these QDs were further modified using CDI, EDC and NHS as mediators in order to increase affinity to BSA (bovine serum albumine) and IgG (imunoglobuline G). Prepared conjugates were characterized by fluorescence spectroscopy (Infinite M200Pro, Tecan) and capillary electrophoresis (Agilent 7100).
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Synthesis of Lanthanidocene Based Compounds
Pospíšek, Marek ; Zmrzlý, Martin (referee) ; Richtera, Lukáš (advisor)
The aim of this bachelor thesis is to summarize the issues of the polymerization catalytic systems based on lanthanidocene complexes. These complexes are subsequently divided by the structure into four basic groups. One part of this work deals with an overview of the most interesting results of the catalytic systems, which are then analyzed and assessed either in its entirety or point of view of catalytic activity, living mechanism of polymerisation and stereo or regioselectivity. The focus is also placed on type of monomers or the conditions of polymerization. In this part of thesis are also described general methods for their synthesis. Found analogies and generalization are commented from the point of view of the design of the catalytic systems for the purpose of choosing the stereo or regioselectivity, molar mass and its distribution. This gives significance to these complexes as catalytic systems for obtaining the required polymer properties. The acquired knowledge is used for the theoretical design of the catalytic system for controlled polymerization.
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