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Transport Properties of Thin Films Based on Polyvinylalcohol
Blažková, Dominika ; Smilek, Jiří (referee) ; Kalina, Michal (advisor)
This bachelor thesis deals with the optimization of the preparation of hydrogel thin films based on polyvinyl alcohol (PVA). The first step was to optimize the preparation of films. PVA, with a molecular weight of 130 kDa, was used for the preparation of films and 10 wt.% solutions in ultrapure water were prepared from it. The heating duration time of the solution was set at 1.5 h at 90 °C. This was followed by drying at 50 °C for 24 h. Subsequently, PVA films were also prepared from solutions of methylene blue in water and from solutions of methylene blue in an aqueous NaCl solution. The prepared films were subsequently characterized by measuring their thickness, swelling ability, specific surface area and diffusion properties. The morphology of the PVA films was also visualized by SEM microscopy. All prepared films were smooth and had a very small specific surface area. The transport properties were monitored by the method of release into solution and Methylene blue was used as a probe. Release into 5, 10 and 20 ml of ultrapure water was observed. The results obtained show that the most methylene blue was released into the solution with the greatest volume and the release of methylene blue from the PVA film takes place freely without significant interaction with the matrix of the PVA film.
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Social Capital of Civil Society Organizations: Interorganizational Relations Across All Sectors
Jirků, Zdeňka ; Matyáš, Ondřej (advisor) ; Šťovíčková Jantulová, Magdaléna (referee)
SYNOPSIS The diploma thesis deals with social capital of Civil Society Organizations, which is introduced here as a strategy for achieving the objectives and mission of Civil Society Organizations through networking and building relations with key players in their surroundings. Through the example of Civil Society Organizations providing services in Tábor the diploma thesis provides insight into the problematic of creation and maintenance of interorganizational relations with stakeholders across all sectors and therefore building external social capital of organizations. The theories of social capital and interorganizational relations with regard to the specifications of Civil Society Organizations are presented in the theoretical part of this thesis. The research part presents findings based on qualitative research, which was conducted through semi-structured interviews with representatives of Civil Society Organizations. The findings and results are and summarized in the conclusion.
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Use of biological materials for tissue substitution in plastic surgery
Měšťák, Ondřej ; Sukop, Andrej (advisor) ; Brychta, Pavel (referee) ; Dražan, Luboš (referee)
Užití biologických materiálů k náhradě tkání v plastické chirurgii ! Abstrakt v angličtině Background: Biological meshes are biomaterials consisted of extracellular matrix and used in surgery particularly for hernia treatment or thoracic wall reconstruction. They are capable of vascularization, that decreases risk of infection, expecially when used in contaminated fields. This study compared the strength of incorporation and biocompatibility of two porcine-derived grafts (cross-linked and non-cross-linked) in a rat hernia model. In addition, we hypothesized that combination of extracellular matrices with autologous mesenchymal stem cells used for hernia repair would result in increased vascularization and increased strength of incorporation. Methods: Standardized 2 x 4 cm fascial defect was created in 42 Wistar rats and repaired with a cross-linked or a non-cross-linked graft either enriched or non-enriched with stem cells. The rats were sacrificed 3, 6 and 12 months later. The strength of incorporation, vascularization, cellular invasion, foreign body reaction and capsule formation were evaluated. Results: Comparison of stem cell enriched and non-enriched groups showed no significant differences in the capsule thickness, foreign body reaction, cellularization or vascularization. In the non-cross-linked...
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Modification of Polyethylene by Reactive Compounding
Horkel, Ondřej ; Kučera, Jaroslav (referee) ; Petrůj, Jaroslav (advisor)
This Master’s thesis deals with the problematics of radically initiated compounding of polyethylene by 1-vinyladamantane. Theoretical part focuses on the theory of grafting processes and how they are influenced by chosen process parameters, as well as the properties of used monomer. In experimental part, two sets of experiments were designed, in both cases the chemicals used were polyethylene LITEN MB 87, (2,5-dimethyl-2,5-bis(tert-butylperoxy) hexane), also called Trigonox 101, as the initiator, and chemicals called Ionol and Irgafos 168 as stabilizers. The experiments were carried out in a Brabender batch mixer, the temperature was 180 – 185 °C, the reaction time was 5 minutes. The analytic methods used to evaluate the prepared samples’ properties were the tensile testing, the Charpy notch test, measuring of the melt flow index, analyzing the torque during kneading and the analysis of infrared spectra of the samples. By carrying out of these experiments it was found that excessive crosslinking was present with mass fraction of initiator being as low as 2 %. It was also concluded that under these conditions the modification does not improve tensile properties of the material, but it does improve the impact strength, albeit slightly. The influence of the modification on flow properties needs to be investigated further. A presence of vinyl groups was observed in infrared spectra, suggesting the presence of ungrafted vinyladamantane.
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Novel Antibacterial Collagen Scaffolds for Regenerative Medicine
Dorazilová, Jana ; Brtníková, Jana (referee) ; Vojtová, Lucy (advisor)
Tato diplomová práce se zabývá charakterizací 3D porézních kolagenových pěn obsahující vybraná antibakteriální činidla. V literární části jsou shrnuty dosavadní poznatky o biomateriálech a biopolymerech se zaměřením na kolagen a chitosan. Dále jsou v ní popsány antibakteriálních vlastnosti nanočástic v kontextu s využitím selenových nanočástic jako antibakteriální činidla. Pro potřeby této práce byla vybrána antibakteriální aditiva dvojího typu – biopolymerní chitosan a selenové nanočástice. 3D porézní struktury bylo dosaženo metodou lyofilizace. Mechanické vlastnosti biopolymerní matrice byly podpořeny síťováním v prostředí chemického síťovadla na bázi karbodiimidu. K charakterizaci připravených kolagenových pěn byly použity především fyzikálně chemické metody. Na analýzu mikrostruktury a velikosti pórů, vizualizaci nanočástic a jejich distribuci v testovaných materiálech byla využita rastrovací elektronová mikroskopie (SEM) spolu s energiově disperzní analýzou (EDX). Parametry jako je porozita, botnací poměr, úbytek hmotnosti při degradaci ve vodném prostředí a v prostředí enzymů byly zjištěny vhodnými gravimetrickými metodami. Ovlivnění chemické struktury kolagenové matrice před a po přidání antibakteriálních aditiv bylo sledováno za pomocí metody zeslabeného úplného odrazu infračervené spektroskopie s Fourierovou transformací (ATR FTIR). Procentuální uvolnění nanočástic z testovaných vzorků bylo sledováno pomocí optické emisní spektroskopie s indukčně vázanou plazmou (ICP OES). K zhodnocení antibakteriálních vlastností testovaných materiálů byly použity především difúzní agarová disková metoda a makrodiluční bujónová metoda. Provedeným výzkumem jsme zjistili vliv přídavku antibakteriálních činidel na strukturu a některé vlastnosti 3D kolagenových matric. Výsledky antibakteriálních testů ukázaly pozitivní vliv chitosanu i selenových nanočástic na inhibici růstu bakterií v závislosti na koncentraci přidaných aditiv. Navržené materiály mohou být využity pro biomedicínské aplikace především v oblastech zabývající se regenerací měkkých tkání.
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Knowledge and networking of Local Action Groups in the Czech Republic in the programming period 2014-2020
Kačerová, Eliška ; Kasáková, Zuzana (advisor) ; Hauser, Jan (referee)
This master's thesis deals with Local Action Groups (LAGs), based on the EU approach LEADER for local development, in the Czech Republic during the programming period 2014- 2020. It focuses on the ability of LAGs to acquire and pass on knowledge and to learn, as well as on their networks. Within this work, the character of acquired knowledge, means of transfer of the knowledge and forms of learning are examined. Furthermore, research focuses on LEADER networks and their internal relations as well as level of communication and trust, in order to determine the efficiency of these networks. Lastly, the institutional thickness of the environment of Czech LAGs is examined that constitutes of non-scientific institutions and technological infrastructure. Cooperation with these two types of institutions leads to creation and implementation of innovations and more effective development of the territory. The theoretical grounding and criteria of this work are based on the Theory of Learning Regions. To obtain the data, a mixed research method was used. A combination of quantitative and qualitative research was carried out in the form of a questionnaire survey, semi-structured interviews and analysis of primary documents.
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Bio-based wood adhesive derived from brewers spent grain
Muselíková, Vladislava
Brewers spent grain (BSG) is an abundant waste material from the brewing process, representing approximately 85% of total by-products generated, is rich in cellulose and non-cellulosic polysaccharides and has a strong potential to be recycled. One of the main components of the BSG is protein, which has a potential to be extracted, modified and used as an adhesive for the woodworking adhesive. This work deals with the protein extraction through alkali treatment and its subsequent modification, crosslinking with glyoxal. The crosslinked adhesive was tested through ABES method to find, that ideal content of glyoxal is 20%. Subsequent adjusted standard tests for the lap shear strength and bend strength (MOE and MOR values) were used to compare its values to commercially available PVAc adhesive. The lap shear strength results were 2,7 times lower to PVAc, the bend strength values were, on the other hand, 1,5 times higher. The PVAc was partially replaced (50 and 25%) by the protein adhesive showing that the extracted adhesive could serve as a partial replacement to lower the ecological impact of the PVAc adhesive.
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HelenOS routing and porting of BIRD
Gálfy, Stanislav ; Děcký, Martin (advisor) ; Mareš, Martin (referee)
Capability to route can be considered as one of the key features of a modern multipurpose operating system, which HelenOS aims to be. The goal of this master thesis is to explore current HelenOS routing capabilities, enhance them and empower HelenOS with BIRD. HelenOS will become a routing operating system with awareness of its surroundings. It will be capable of dynamic adaptation to changes in the network and their propagation.
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Use of starch and glycerin in polymer films with controlled life span
Almásyová, Zuzana ; Pospíšil, Ladislav (referee) ; Jančář, Josef (advisor)
This diploma thesis deals with preparation and testing of biodegradable materials for use in the packaging indrustry. The task of this work is to prepare set of laboratory made films based on wheat starch, glycerol, polyvinyl alcohol with the addition of a suitable additive, which has been selected as citric acid. A suitable composition meeting the requirements of the packaging foils has been found. Quaternary blends from all components achieve the best results. It has been found that citric acid can influence the chemical structure and thus affect the mechanical and thermal properities and also the degree of solubility. The films were characterized in terms of mechanical properities by tensile testing and thermal stability by means of thermogravimetric analysis with respect to the phase structure. The structure was characterized by Fourier transform infrared spectroscopy and confocal laser scanning microscopy. The biodegradability of the film was tested by its solubility in aqueous medium.
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HelenOS routing and porting of BIRD
Gálfy, Stanislav ; Děcký, Martin (advisor) ; Mareš, Martin (referee)
Capability to route can be considered one of key features of modern multipurpose operating system, which HelenOS aims to be. Goal of this master thesis is to explore current HelenOS routing capabilities, enhance them and empower HelenOS with BIRD. Thanks to BIRD, HelenOS will become a routing operating system, that is aware of its surroundings and is capable of dynamic adaptation to changes in network, it is part of.
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