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Řízená fermentace pomocí sledování produkce oxidu siřičitého
Osička, Dominik
The bachelor thesis is concerned with the problem of controlled fermentation by monitoring carbon dioxide and sensory evaluation of three wines. These wines had different fermentation conditions. The theoretical part of the thesis is about alcoholic fermentation and its products. These part of text also describes biosensors and sensors use in oenology. The practical part includes a description of the variety Gewürztraminer. There are described a processes and methods of production of wines and a set of analytical and sensory methods which were performed. Results of individual wines are described in the end of work.
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The application of electrochemical impedance spectroscopy for investigation of enzyme kinetics
Pospíšilová, Michaela ; Sedláček, Jiří (referee) ; Fohlerová, Zdenka (advisor)
The application of electrochemical impedance spectroscopy (EIS) in theory and experiment for investigation of redox enzyme kinetics has been already described. The impedance studies are quite sensitive to the changes at the interface caused by adsorption of charged species and therefore can be used to study the kinetics of adsorption of macromolecules and also enables us to study enzyme kinetics since the action of the enzymes on their substrates involved modification and generation of charged species. In this work, we would explore the potential of electrochemical impedance technique to follow the kinetics of glucose oxidasesubstrate reactions on the immobilized surface. The enzyme will be allowed to interact with different concentrations of its substrate and the resulting reaction will be recorded in real time. Changes in the imaginary component of the impedance at various substrate concentrations will be expected to follow MichelisMenten kinetics. The experimental procedure should be simple and can be carried out directly in buffer solutions most suited for the required interaction to take place, without the requirement of any additional redox probes.
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Organic tranistors for sensorics
Marková, Aneta ; Omasta, Lukáš (referee) ; Vala, Martin (advisor)
The aim was to find the best available commercial formulation of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) and methods and subsequent treatment of thin films for organic electrochemical transistors (OECT). These films will be used in bioelectronic sensors to measure the physiological function of cardiomyocytes. Studied material was PEDOT:PSS in six different formulations. Thin layers prepared by spin coating were modified in three ways, namely (i) immersion in ethylene glycol, (ii) drying and (iii) a combination of drying and ethylene glycol. The layers were then tested for the influence of ethylene glycol, thermal stability, the impact of storage, influence of aqueous medium, and additional thermal annealing. According to the results obtained, the most suitable treatment of most materials was immersion in ethylene glycol followed by thermal annealing of the layer. It was found that the most appropriate material formulation was Sigma Aldrich 739 316 treated by the combination of drying, immersion in ethylene glycol and thermal annealing.
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Diagnostics of genom conditioned diseases with the use of micro- and nanoparticles
Mondeková, Věra ; Prášek, Jan (referee) ; Provazník, Ivo (advisor)
The bachelor´s thesis discusses possibilities of viral genome´s detection through use of biosensors, more specifically through use of magnetic particles. The introductory part consists of brief characteristic of viruses, mentioned as originator of genom conditioned diseases, followed by chapters related to selected methods of nucleic acid´s extraction and analysis. The main part is dedicated to magnetic particles. The practical part of thesis deals with possibility of use of biosensors in specific viral pathogen´s detection, selection of biocompatible molecules suitable for magnetic particle modification and description of specific DNA sequence isolation procedure through use of magnetic particles.
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Magnetic particles as a reversible carrier for enzyme electrodes
Janíček, Zdeněk ; Žeravík,, Jiří (referee) ; Skládal,, Petr (advisor)
Master’s thesis contains information about the enzymes, biosensors, enzyme biosensors and magnetic particles. Cholinesterasa (ChE) is the generally name for the two related enzymes, mutually differing appearance and biological functions. Acetylcholinesterasa (AChE) is necessary for the transmission of nervous excitement. AChE is located at cholinergic synapses, where is the hydrolysis of the neurotransmitter acetylcholine and this termination by the nervous excitement. Butyrylcholinesterasa (BChE) is related to AChE and it is present in serum. Experimental part was focused on electrochemical biosensors with cholinesterase enzyme using magnetic particles for reversible reconstruction layer. Cholinesterase immobilization was carried out on the magnetic particles by covalent binding after glutaraldehyde activated. The measurement of activity take place with acetylthiocholin as a substrate in a flow system, magnetic particles were captured before the platinum electrode and produced by thiocholin is detected amperometric. The aim was to measure inhibition of AChE. Significant inhibition ChE is caused by certain drugs and pesticides based on organophosphates. There was tested by inhibition of AChE pesticide dichlorvos. Emphasis was on finding possible experimental conditions for the creation of the automated procedure to measure the AChE activity, which was based on auto-injector and pumps of Gilson, together with a 735 programme for the definition of the working configuration, preparation of measurement scripts and implementation of application methods.
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Natural patterns in robotics
Juřicová, Vendula ; Augste, Jan (referee) ; Knoflíček, Radek (advisor)
This bachelor´s thesis is focused on the part of the robotics, which is inspired by nature. At first, the reader is introduced into the theory of interdisciplinary terms BIO – ING. The central part is divided in two parts: the inspiration by man and inspiration by animals. In the chapter about man, there are the main biomechanical features introduced as well as the algorithms that are used in the robotics with the human prototype. Some biomechanical constructions and humanoid robots follow. Biomechanical constructions that are inspired by animals dominate the second part. The conclusion includes (among others) a technical and a philosophical reflection on the future of the robotics.
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Functionalized nanostructures
Váňa, Rostislav ; Kvapil, Michal (referee) ; Kolíbal, Miroslav (advisor)
This thesis deals with functionalized nanoparticles. In the first part there are mentioned materials suitable for a functionalization, the usage of functionalized nanoparticles in medicine and biochemistry and detection methods of changes of optical properties. In the second part changes of optical properties after functionalization are investigated by spectroscopic ellipsometry and FTIR spectroscopy.
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