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Patterning of excitable cells on multi-electrode arrays
Slavík, Jan ; doc. RNDr. Petr. Skládal, CSc. (referee) ; Průcha,, Jaroslav (referee) ; Hubálek, Jaromír (advisor)
Práce se zabývá zarovnáváním excitabilních buněk na multielektrodových polích. Nejprve bylo analyzováno zarovnávání excitabilních buněk. Byly použity embryonální neurony z hippocampusu potkanů a HL-1 buňky, které jsou odvozeny z AT-1 linie nádorových myších atriálních kardiomyocytů. Zarovnávání bylo testováno na drážkovaných površích a na površích s materiály s různou buněčnou afinitou. Bylo prokázáno, že na drážkových površích se ve směru drážek zarovnávají neurony i HL-1 buňky, ale na površích s různou chemickou affinitou se zarovnávají pouze neurony. Dále byly vyrobeny vlastní multielektrodová pole, na těchto multielektrodových polích byly kultivovány HL-1 buňky a byl změřen a analyzován akčních potenciál HL-1 buněk. Cílem bylo prokázat, že je možné měřit akční potenciál na vyrobených multielektrodových polích. Pro zarovnání buněk na multielektrodovém poli bylo vyrobeno speciální multieletrodové pole s uniformním povrchem. Toto multielektrodové pole je nazýváno planární multielektrodové pole. Planární multielektrodové pole bylo vyrobeno speciálním vyrobním procesem. Vrstvy planárního multielektrodového pole byly deponovány na pomocný substrát v opačném pořadí. Pomocný substrátem pro depozici byla křemíková deska, na který byla nadeponována další pomocná vrstva zlata. Horní izolační vrstva planárního multielektrodové pole byla deponována jako první a nejspodnější vrstva substrátu byla nadeponována jako poslední. Planární multielektrodové pole i s pomocnou zlatou vrstvou bylo strhnuto s křemíku díky nízké adhezi zlata ke křemíku a planární multielektrodové pole se otočilo vzhůru nohama. Pomocná zlatá vrstva byla odstraněna mokrým leptadlem a tím bylo planární multielektrodové pole dokončeno. Na planárním multielektrodovém poli byly zarovnány HL-1 buňky do pruhů chemickou metodou pomocí kombinace otisku adhezní látky a následným potažení neotisklých ploch anti-adhezní látkou. Elektrofyziologické vlastnosti zarovnaných HL-1 buněk byly změřeny pomocí planárního multielektrodového pole. Tímto experimentem byla představena výrobní technologie pro výrobu planárních multielektrodových polí a toto planární multielektrodové pole bylo úspěšně testováno pro zarovnání HL-1 buněk na jeho povrchu kombinací otisku adhezní látky a potahování antiadhezivním činidlem.
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Adhesion of Adhesive Joints for Facade Applications Made of Wood-based Materials
Nečasová, Barbora ; Schmid, Pavel (referee) ; Sedlák,, Petr (referee) ; Kovářová, Barbora (advisor)
The presented dissertation thesis is focused on investigating the adhesive properties of façade cladding made of wood and wood-based materials in combination with flexible adhesion/assembly systems. The systems allow the implementation of effective construction joints and provide a solution with uniform stress distribution which allows the transfer of heavy loads, while simultaneously reducing the weight of the entire structure, which is also often connected with substantial cost reductions. Despite all that, little attention has been paid to this area so far. It is not a solution which is properly embodied in technical standards or in designers´, architects´ and contractors´ awarenesses. The aim of this dissertation is to characterise adhesive properties of selected façade cladding representatives made of wood and wood-based materials for the purposes of bonded joint application in a ventilated façade system. To define adhesion of façade joints, numerical and experimental methods are used, taking also various limit conditions into account. The main conclusion of the dissertation is the characterisation of basic parameters which influence adhesion, effectiveness and quality of façade glued joints. The secondary conclusion is the compilation of a simple methodical procedure for testing and implementing façade glued joints. The dissertation also makes a contribution by extending the current state of knowledge of façade adhesive joints.
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The study of adhesion of glued balistic composites on the dependence of materials used.
Dobiáš, Jiří ; Másilko, Jiří (referee) ; Šiler, Pavel (advisor)
The aim of this work was to investigate the properties of bonded ballistic materialsBalistic composites are mainly glued to reduce weight while keeping their protective properties. On this basis, the materials used were ballistic steel, ceramics, rubber kevlar, non-gummed kevlar, Dyneema and fiberglass. For bonding used were a one-component adhesive Collano 36.104 and two-component adhesive Biresin U1305. The strength of the joint was measured in shear at tensile loads. From these results, deformation energy was further calculated.
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Effect of corrosion environment on the long-term stability of composite material to enamel and dentin bonds
Dudek, Michal ; Bradna, Pavel (advisor) ; Veverka, Jan (referee) ; Halačková, Zdena (referee)
Introduction: Adhesive technology represents a significant advancement among different treatment options of dental caries. Its advantages involve not only highly aesthetic reconstructions, but also ability to preserve hard dental tissues compared to more traditional filling materials such as amalgam, where the material retention was achieved through undercuts. A potential of adhesive technology to arrest progression of an incipient caries has triggered its wider use in dental caries management, potentiated by an ongoing development of new adhesive systems and composite materials that assure an acceptable long-term stability of adhesive reconstructions. Adhesive bonds of dental reconstructions are in the oral cavity constantly challenged by various mechanical, chemical and biological factors. One of the chemical agents with detrimental potential is peroxide bleaching gel used for teeth brightening. Currently bleaching belongs to the most frequent cosmetic dental procedures. The active ingredient of bleaching gels is hydrogen peroxide or its precursors. It is assumed that free oxygen radicals released from peroxides attack double bonds of chromophore molecules captured within the tooth tissues. Because of the high reactivity and nonspecific nature of these oxygen radicals, they may affect also dental...
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Study of durability of wood glue bonds against increased temperature
Kučera, Vít ; Vejpustek,, Zdeněk (referee) ; Vaněrek, Jan (advisor)
In this time are for design of wooden elements available simplified procedures for fire modeling which defines ČSN EN 1991-1-2. But it is not experimentally tested the impact of adhesive on the final value of fire resistance of laminated panels. This diploma thesis solves basic normative practices that could explain the durability of glue bond against increased temperatures. in the practical part will be undertaken to experimentally assess the impact of various kinds of adhesive-based (epoxy, formaldehyde, PUR and EPI) on the glued bond durability against increased temperature. Glued joints that will be subjected to gradual temperature exposure in order to assemble the dependence of the temperature influence on the final strength of the joint according to the type of adhesive.
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Resistance of waterproofing systems for flat roofs
Kerekanič, Robin ; Žák,, Antonín (referee) ; Žižková, Nikol (advisor)
This bachelor thesis targets on the resistance of waterproofing systems for flat roofs using bitumen sheets. It proposes an alternative methods for testing the adhesion of the waterproofing on warm flat roof. Endpoint is the adhesion of self-adhesive bitumen sheet for thermal insulator made of expanded polystyrene to the effects of static and dynamic stresses. Finally, it assessed the appropriateness of the methods used and the effect of the adhesion of self-adhesive bitumen sheet on the method of execution.
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Heat resistance of wood bondlines
Šustáček, Jan ; Melichar, Tomáš (referee) ; Vaněrek, Jan (advisor)
This thesis is focused on studying the impact of elevated temperatures on the resistance of bonded joints. The aim of this study is to assess the impact of exposure to temperatures on selected types of adhesives. In the theoretical part was made research and comparison of selected species agglomerated materials and described the issue of fire safety of buildings. To determine the strength characteristics of bonded joints at different temperatures have been made in the practical part load tests according to valid standards.
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Repair of surface sliding
Teplý, Miroslav ; Vyplašil, Evžen (referee) ; Kubíček, Jaroslav (advisor)
The selection of suitable materials for the renovation of sliding shafts requires a comparison of several criteria. The main criteria were selected size shear strength under tensile stress lap bonded assemblies, resistance to adhesive wear and economic criteria. From the results of all tests is selected the most suitable friction material to repair worn out places of shaft. The technological process of sealing the shaft was drawn up on the basis of the literature, recommendations supplier of sliding materials and their own experience.
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Effect of surface on bonded joint
Trhoň, Vojtěch ; Halamík, Tomáš (referee) ; Kubíček, Jaroslav (advisor)
This master’s thesis is divided into two parts. In the first part are these topics: bonding theory, treatment of bonding surface, types of adhesives, adhesives in the transportation industry and construction and stress of bonded joints. In the second part of this thesis is experimental evaluation of the effect of surface of material on strength of bonded joint.
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