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Genotoxicita jako kriterium účinnosti biodegradace barviv v odpadních vodách
Doležílková, I. ; Pavlíčková, Z. ; Malachová, K. ; Lednická, D. ; Novotný, Čeněk ; Šušla, Martin
The aim of this study was to evaluate the mutagenicity of three mixtures of synthetic dyes used in textile industry. Detection of mutagenicity was carried out with three samples containing residues of textile dyes obtained from textile company Jitex Písek. Two different biodegradation process were used for elimination of genotoxicity, biodegradation using the bacterial activated sludge and the enzyme systém of the ligninolytic fungus Irpex lacteus. The genotoxicity of the samples was detected by the Ames test with and without metabolic activation in vitro, using S9 liver microsomal fraction and cofactor mixture. Salmonella typhimurium His- strain TA98 was employed for detection of frameshift mutations in the Ames test. During two different biodegradation processes (using bacterial activated sludge and the ligninolytic fungus Irpex lacteus), the change of mutagenicity of the degradation products of dyes was monitored
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