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The role of histone deacetylase 6 in murine polyomavirus replication cycle
Vlachová, Štěpánka ; Horníková, Lenka (advisor) ; Saláková, Martina (referee)
The replication cycle of polyomaviruses is, consistently with other viruses, fully dependent on host cells. Not only the cellular replicational and translational mechanisms are important for viruses, but also the virus infection is affected by other cellular proteins. This work is focused on the role of major cytoplasmic deacetylase, histone deacetylase 6 (HDAC6) in replication cycle of murine polyomavirus (MPyV). We showed that the presence of fully functional HDAC6 is essential for successful and productive infection. We found that HDAC6 affects not only early phase, but also late phase of infection. Cells with inhibited, or absent HDAC6 are infected with decreased effectivity and moreover lower amount of infectious viral particles is produced. On the other side, using cells with partially functional HDAC6, either in its deacetylase activity or in ubiquitin-binding activity, leads to increased ability of MPyV to infect those cells. Analysis of levels of early LT antigen and late structural protein VP1 in the infected cells showed, that viral proteins are affected by HDAC6. Our data suggest, that in the replication cycle of MPyV mainly the ubiquitin-binding domain of HDAC6 is required and the role of this domain in protein metabolism and degradation. In the second part of diploma project, we...
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Mechanisms of superinfection exclusion by animal viruses
Vlachová, Štěpánka ; Horníková, Lenka (advisor) ; Šmahel, Michal (referee)
Viral interference can be found in both homologous and heterologous viruses and may have significant impact on the infection. One of such impacts is superinfection exclusion, which is a defence mechanism used by a wide variety of viruses. The superinfection exclusion may occur in different steps of viral life cycle. Despite being a common viral function, most of the mechanisms of exclusion stay yet undefined, same as proteins, which are taking part in superinfection exclusion. This work is a summary of knowledge in the field of interference between animal viruses, with focus on homologous interference and mechanisms of superinfection exclusion. Research of this phenomenon can bring important information for the development of new antiviral treatment, virus diagnosis and understanding of interactions between viruses. Key words: Viral infection, animal viruses, homologous interference, superinfection exclusion, mechanisms of exclusion
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