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Novel binding proteins derived from small protein domains targeting diagnostically important molecules
Vaňková, Lucie ; Malý, Petr (advisor) ; Brynda, Jiří (referee) ; Trefil, Pavel (referee)
The rapid development of the gene engineering techniques, especially methods for in vitro directed evolution and combinatorial mutagenesis, has triggered the generation of new binding agents to almost any antigen of interest as an alternative to broadly used antibodies. These so-called non-Ig scaffolds are often derived from proteins with useful biophysical properties. While the therapeutic market is still dominated by monoclonal antibodies, the easy option of desired customization of non-Ig binders by conventional methods of gene engineering predestine them largely for the use in the diagnostic area. The ABD scaffold, derived from a three-helix bundle of albumin-binding domain of streptococcal protein G, represents one of the small non-Ig scaffolds. In our laboratory, we have established a highly complex combinatorial library developed on the ABD scaffold. This ABD scaffold-derived library was used to generate unique binders of human prostate cancer (PCa) biomarkers PSP94, KLK2, KLK11 for the more precise diagnosis of PCa. The second part of the thesis describes the generation of ABD-derived binders selectively recognizing different phenotypes of circulating tumor cells as a binding component of the cell capture zone of microfluidic chip for lung adenocarcinoma diagnosis. Beside this already...
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Spiritual care for persons with mental disorders
Trefil, Petr ; Opatrný, Aleš (advisor) ; Kuźniar, Mariusz (referee)
Anglická anotace "Spiritual care for persons with mental disorders" The aim of bachelor thesis is concerned about the spiritual needs of man in health and disease. Attention is paid to their place in the life values, with particular emphasis on the psychological state of the disease. The author outlines the basic principles of the pastoral care for selected types of the most common mental disorders, the possibility of satisfying the spiritual needs in psychiatric facilities and activities of the layman in pastoral care. Key words: spiritual needs, mental disorders, psychiatric facilities, basic principles of the pastoral care, layman in pastoral care
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Variability of the domestic chicken breeds in selected immunological traits of hen and egg
Bílková, Barbora ; Vinkler, Michal (advisor) ; Plachý, Jiří (referee) ; Trefil, Pavel (referee)
The avian immune system is a complex system of defence mechanisms that protect bird hosts against threats from ubiquitous pathogens. According to the co-evolutionary models, variability in immune traits of hosts is the key component providing ability to adapt and enhance their defence mechanisms in presence of constant selective pathogen pressure. Domestic chicken (Gallus gallus f. domestica) is used as a model organism in avian biology and also is one of the most important food-producing animals, not only for their meat but also for the egg production. Unfortunately, in research usually only inbred chicken lines are used and modern poultry husbandry is tight with unilateral breeding towards highly productive breeds. Those approaches decrease intra-population polymorphism in chickens. However, especially in case of farm animals, searching and extending the pool of immune variability and enhancing pathogen resistance is crucial for sustaining healthy and biologically secure populations and their products. Morphologically highly distinct traditional chicken breeds, which have evolved for hundreds years under different selective pressures, may represent this desirable immunological variability. In my thesis I described variability in chosen immunological traits, haematological parameters and proteomic...
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Novel binding proteins derived from small protein domains targeting diagnostically important molecules
Vaňková, Lucie ; Malý, Petr (advisor) ; Brynda, Jiří (referee) ; Trefil, Pavel (referee)
The rapid development of the gene engineering techniques, especially methods for in vitro directed evolution and combinatorial mutagenesis, has triggered the generation of new binding agents to almost any antigen of interest as an alternative to broadly used antibodies. These so-called non-Ig scaffolds are often derived from proteins with useful biophysical properties. While the therapeutic market is still dominated by monoclonal antibodies, the easy option of desired customization of non-Ig binders by conventional methods of gene engineering predestine them largely for the use in the diagnostic area. The ABD scaffold, derived from a three-helix bundle of albumin-binding domain of streptococcal protein G, represents one of the small non-Ig scaffolds. In our laboratory, we have established a highly complex combinatorial library developed on the ABD scaffold. This ABD scaffold-derived library was used to generate unique binders of human prostate cancer (PCa) biomarkers PSP94, KLK2, KLK11 for the more precise diagnosis of PCa. The second part of the thesis describes the generation of ABD-derived binders selectively recognizing different phenotypes of circulating tumor cells as a binding component of the cell capture zone of microfluidic chip for lung adenocarcinoma diagnosis. Beside this already...
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Variability of the domestic chicken breeds in selected immunological traits of hen and egg
Bílková, Barbora ; Vinkler, Michal (advisor) ; Plachý, Jiří (referee) ; Trefil, Pavel (referee)
The avian immune system is a complex system of defence mechanisms that protect bird hosts against threats from ubiquitous pathogens. According to the co-evolutionary models, variability in immune traits of hosts is the key component providing ability to adapt and enhance their defence mechanisms in presence of constant selective pathogen pressure. Domestic chicken (Gallus gallus f. domestica) is used as a model organism in avian biology and also is one of the most important food-producing animals, not only for their meat but also for the egg production. Unfortunately, in research usually only inbred chicken lines are used and modern poultry husbandry is tight with unilateral breeding towards highly productive breeds. Those approaches decrease intra-population polymorphism in chickens. However, especially in case of farm animals, searching and extending the pool of immune variability and enhancing pathogen resistance is crucial for sustaining healthy and biologically secure populations and their products. Morphologically highly distinct traditional chicken breeds, which have evolved for hundreds years under different selective pressures, may represent this desirable immunological variability. In my thesis I described variability in chosen immunological traits, haematological parameters and proteomic...
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Relatedness and gene flow between breeds of the domestic fowl
Buchtová, Lucie ; Vinkler, Michal (advisor) ; Trefil, Pavel (referee)
Domestic chickens are domestic animals with tremendous economic value. Due to this value, high-production lines have more important role for humans than fancy breeds and rural populations. Production traits of these high-production lines are not comparable with traditional breeds. The preference for larger profit gradually diminishes the importance of the traditional breeds, which leads to their replacement increasing the risk of loss of their genetic variability. Despite this trend, general awareness of this problem increses providing hope in saving these rich sources of genetic variation. We selected sample of 272 individuals representing 33 traditional (fancy) breeds. I evaluated the degree of variability through the analysis of twenty selected neutral markers (mtDNA control region and 19 microsatellite loci) and four immune genes (TLR3, TLR4, TLR5, TLR7). The main aims were: to map the origin of modern breeds between ancient breeds, identify the possible geographic origin of the breeds, to compare the rate of neutral and functional variation between breeds with the level of variation within breeds and to compare the degree of shared polymorphism (gene flow / ancestral polymorphism) of neutral markers and immune genes. The results show that we can genetically differentiate breeds and identify...
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Spiritual care for persons with mental disorders
Trefil, Petr ; Opatrný, Aleš (advisor) ; Kuźniar, Mariusz (referee)
Anglická anotace "Spiritual care for persons with mental disorders" The aim of bachelor thesis is concerned about the spiritual needs of man in health and disease. Attention is paid to their place in the life values, with particular emphasis on the psychological state of the disease. The author outlines the basic principles of the pastoral care for selected types of the most common mental disorders, the possibility of satisfying the spiritual needs in psychiatric facilities and activities of the layman in pastoral care. Key words: spiritual needs, mental disorders, psychiatric facilities, basic principles of the pastoral care, layman in pastoral care
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Outsourcing metodology in Konica Minolta ltd.
Trefil, Pavel ; Bruckner, Tomáš (advisor) ; Koutný, Stanislav (referee)
Cílem diplomové práce bylo navrhnout metodiku poskytování outsourcingu v oblasti tiskových a reprografických služeb z pohledu společnosti, která outsourcing poskytuje. . Navržená metodika reflektuje proces outsourcingu formou projektu. Práce se skládá ze čtyř částí. První část se zabývá obecným vysvětlením pojmu outsourcing, jeho přínosy, výhodami a nevýhodami. Druhá část se věnuje zhodnocení současného stavu outsourcingu tiskových služeb ve firmě Konica Minolta a navržení zlepšení, kterých je možno dosáhnout. Ve třetí části je vypracován návrh metodiky poskytování outsourcingu v Konica Minolta. V poslední, čtvrté části, je pro přiblížení situace na trhu a pro možnost srovnání uvedena nabídka služeb největších konkurentů společnosti.
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