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Localization of double bond positions in triacylglycerols using HPLC/APCI-MS2
Háková, Eva ; Cvačka, Josef (advisor) ; Lísa, Miroslav (referee)
Triacylglycerols are among the most abudant classes of lipid. Their chemical, physical and biological characteristics depend on degree of unsaturation and positions of double bonds in acyls. This thesis was focused on localization of double bonds using tandem mass spectrometry with atmospheric pressure chemical ionization. We studied 14 standards and 44 standard mixtures with different number of double bonds and lenght of hydrocarbob chain in acyls. Standards were obtained from commercial sources and prepared of randomizations reaction in microscale. Localization of double bonds was deduced from fragments of molecular aduct with C3H5N+· ([M+55]+·) created in presence of acetonitrile in the mobile phase. This metod was applied for HPLC/MS analysis of natural mixtures of triacylglycerols. Key words: triacylglycerols, randomization, localization of double bonds, atmospheric pressure chemical ionization, mass spectrometry, high performance liquid chromatography with mass detection
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Localization of double bond positions in triacylglycerols using HPLC/APCI-MS2
Háková, Eva ; Cvačka, Josef (advisor) ; Lísa, Miroslav (referee)
Triacylglycerols are among the most abudant classes of lipid. Their chemical, physical and biological characteristics depend on degree of unsaturation and positions of double bonds in acyls. This thesis was focused on localization of double bonds using tandem mass spectrometry with atmospheric pressure chemical ionization. We studied 14 standards and 44 standard mixtures with different number of double bonds and lenght of hydrocarbob chain in acyls. Standards were obtained from commercial sources and prepared of randomizations reaction in microscale. Localization of double bonds was deduced from fragments of molecular aduct with C3H5N+· ([M+55]+·) created in presence of acetonitrile in the mobile phase. This metod was applied for HPLC/MS analysis of natural mixtures of triacylglycerols. Key words: triacylglycerols, randomization, localization of double bonds, atmospheric pressure chemical ionization, mass spectrometry, high performance liquid chromatography with mass detection
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Nursing process in seniors suffering from pain
HÁKOVÁ, Eva
Abstract Ageing is an irreversible and nonrecurring biological process. Old age is the final stage of life. It is characterized by involutional and mental changes. Ageing has a significent impact on the social status of a person. The typical attribute of old age is polymorbidity. The aged often suffer from chronic ailment that go together with pains. Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage. Nociception is the physiological event that is accompanied by pain. Nonpharmaceutical therapies affect human mind and thus help to ease pain. These techniques enhance the quality of life and make life more comfortable. The aim of my thesis was to make a survey of nonpharmaceutical inhibition of perception of pain at the nursing process in seniors staying at retirement homes or nursing homes. Using a method of questionnaires as a quantitative research, I focused on the question of using alternative, nonpharmaceutical method of pain management after the previous assessment of a patient´s condition. To different medical care facilities, 120 questionnaires were handed out. The 85 questionnaires that were filled in correctly were used for evaluation . The respondents were selected at random {--} 41 nurses from homes for seniors and 44 nurses working in long-term care facilities. From the Hvizdal retirement home I received only 13 questionnares I could assess and from the Maj retirement home only 16 nurses handed in the questionnaires filled in correctly. I was given 12 correctly filled questionnaires from the retirement home in Jicin and 28 from the Gerontcentre in Hostinne. The total number of 85 correctly filled questionnaires is completed by those 16 from nurses working in the long-term care facility in Lomnice nad Popelkou. The objective of my thesis has been achieved. The hypothesis H I {--} nurses working with seniors in medical care facilites use nonpharmaceutical therapies after the previous assessment of a patient´s condition {--} was confirmed. The hypothesis H II {--} nurses use nonphamaceutical therapies without the previous assessment of a patient´s condition {--}was not confirmed.
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