|
|
Biosynthesis of terpenoid secondary metabolites in termites
Jungwirth, Daniel ; Heidingsfeld, Olga (advisor) ; Buček, Aleš (referee)
Terpenoids are natural compounds, which can be found in the form of tens of thousands diverse molecules in all forms of life. Terpenoids are mostly represented in plants, microorganisms,and animals. It should not be a surprise that terpenoids are often found in insects, the most species-rich class of Metazoa. In insects terpenoids are mostly used for communication as pheromones and for chemical defence. In comparison to widespread knowledge of terpene (and terpenoid) biosynthesis in plants we lack thorough knowledge in insects. Studies conducted in past few years showedthat the ability to synthesize terpenoids has occurred independently and repeatedly in various lineages of insects. It was also found that terpene synthase genes evolved via duplication and neofunctionalization of specific genes, which were responsible for isoprenyldiphospate synthesis. Despite these discoveries the terpenoid synthesis in insects is mostly unknown and remains to be elucidated. This thesis aims to functionally characterize three candidate terpene synthases from the termite Nasutitermes takasagoensis. Expression of selected proteins was done in two bacterial systems. For the purification of recombinant proteins immobilized metal ion affinity chromatography was used. Detection of products from assays with purified...
|
|
|
Advanced Techniques Demo Using OpenGL
Buček, Antonín ; Přibyl, Jaroslav (referee) ; Herout, Adam (advisor)
This thesis demonstrates advanced techniques using OpenGL. The work is conceived as a demo without any size restrictions. It focuses on creating 3D world, which consists of inner rooms complex. Some additional interesting objects, lighting and shadows enrich the rooms. Particle systems, which were used to create explosions, smokes and some other particle effects are described in the thesis, too. 3D machine models fight between themselves using laser beams and form the story of the demo. Important part of the work is a movement model which is applied to both models and avatar. Sound track completes the demo.
|
|
| |
|
| |
|
|
Dynamics of alpine timberline in the High Sudetes
Treml, Václav ; Lipský, Zdeněk (advisor) ; Jeník, Jan (referee) ; Buček, Antonín (referee)
L.Jl: E BEZ : -P EE .éE 1 ;EÉEĚEE gE E €É EěEťĚl ůÉ+.šqÉE ťšÉiE ;EEFF; €ÉšsĚs gg€g g;Ěc *EegáÉ ÉB;g,Et;EgĚ ;freĚ pĚĚšáĚ iěřEiiE.ř:tg E.ĚEE- E gĚ$EE i€E e EqlttsĚcEÉgĚEĚEEĚBĚa.É€EÉÉ;ťěťEĚ:E šEEEBE u;-:'*s aEs€ šÉÉgEÉ -E-- ',= i"! -=: ::: , ;^=;:.=;:::!,=!=:i= .= =r.9=il=.;í:= i::=,=7=.:Z_,:..:.= = -^:'= =c =.;ž:.==.==:".=-=* : = = := = : : - ;: - -ž-== : :i:==:=:ii:===€= ==i -. = ? . 2 - ..'. =+ = := :l= =:-:4 =. !:ř-rí- :; =.,=ž.ž i'i=-. ž=a = i 7:ž: =ž==ž lii::: ž:=ž=,=, =:=zi = - =. j.Ě2 ':='niž=i4':= :ra =- -:1:1 =1= == -', = = = ,'=7 =-2 - -:: a 1' ' .: ==. >: .. : = ]= = ;: ? ži,== .;E : = ==: :=-,=== . !:1== 11=z; ; : 11z t=':,iž=.== = : =i :;'2 :ž='= =? zi = i: ; * 2,:i7+š =;=.- i Ť ž4 : i= ?E = Et=s; ř É€ E i á: HE * i € E ÉšE Ť g= E 5.:-ž=,, : B E řP *aÉ = ř€E $fr-UE;š e:= E?E E.qE = 3t;; -Ěč g í;"q€.Ei E =E !iEs ;E E;:ň€ĚEšE ĚŤ eřE fi :c E Pn: l : E€-.E,p p gE E EE€EŤ.=ŤsŘ E apu* E ř*E BÉĚF€ EěEř€ q€ EEi E3! EERE gEŠ:Eť E Ě ; g t c E [ á g PE s: =. n 3 3 E š6Ě € E i a*.! = o a"r E č€? Ež=E 3>šE }EE 8 $3 g 9EůE;R.sE ; ď;E
|
|
| |
|
|
Study of evolution of insect pheromone biosynthetic fatty acyl desaturases
Buček, Aleš
Insects account for more than one million of described species with an ecological and economic impact disproportional to their minute body size. Among the factors which have contributed to their evolutionary success, insect secondary metabolites such as defensive compounds and chemical signals are regarded to play a major role. This thesis aims at uncovering the molecular mechanisms underlying evolution of ubiquitous insect secondary metabolites - sex pheromones (SPs), i.e. chemical signals mediating mate finding and mating between individuals of the same species. The thesis focuses on a class of oxidoreductase enzymes, membrane fatty acid desaturases (mFADs), which introduce double bonds into hydrocarbon chains of fatty acyls and thus produce precursors of unsaturated fatty acid- derived SPs. mFADs are involved in SP biosynthesis in e.g. moths (Lepidoptera), flies (Diptera), cockroaches and termites (Blattodea), wasps and bees (Hymenoptera) - some of the most species-rich insect orders. Since SPs are principal to species reproductive isolation, uncovering the molecular basis of insect SP biosynthesis holds promises to contribute to answering fundamental questions concerning the insect ecology and evolution. The insect mFADs with diverse enzymatic specificities also represent a naturally available...
|
|
|
The optimization of forest management strategies on slopes affected by landslides and rock falling – a methodology for forest-planting practices
Bajer, A. ; Balková, M. ; Buček, A. ; Divíšek, Jan ; Friedl, M. ; Halas, Petr ; Kirchner, Karel ; Kuda, František ; Lacina, Jan ; Martiník, A. ; Roštínský, Pavel ; Simon, J. ; Vavříček, D.
Landslides, as well as rock falling, are among natural disturbances, the origin of which the forest management has only a secondary influence. On the other hand, adequate management can reduce the risk of landslides (rock falling). In some specific cases, it can be expected that this management can also prevent origin of landslides. The basic recommendation in the area of forest cultivation in areas affected by landslides is the creation of forest stands of species, age and thickness (height) differentiated, with a small-scale texture. In some cases it is also possible to recommend the shape of the associated forest. Consistent management of the forest transport network must be an integral part of measures in risk areas.
|
|
|
Dynamics of alpine timberline in the High Sudetes
Treml, Václav ; Lipský, Zdeněk (advisor) ; Jeník, Jan (referee) ; Buček, Antonín (referee)
L.Jl: E BEZ : -P EE .éE 1 ;EÉEĚEE gE E €É EěEťĚl ůÉ+.šqÉE ťšÉiE ;EEFF; €ÉšsĚs gg€g g;Ěc *EegáÉ ÉB;g,Et;EgĚ ;freĚ pĚĚšáĚ iěřEiiE.ř:tg E.ĚEE- E gĚ$EE i€E e EqlttsĚcEÉgĚEĚEEĚBĚa.É€EÉÉ;ťěťEĚ:E šEEEBE u;-:'*s aEs€ šÉÉgEÉ -E-- ',= i"! -=: ::: , ;^=;:.=;:::!,=!=:i= .= =r.9=il=.;í:= i::=,=7=.:Z_,:..:.= = -^:'= =c =.;ž:.==.==:".=-=* : = = := = : : - ;: - -ž-== : :i:==:=:ii:===€= ==i -. = ? . 2 - ..'. =+ = := :l= =:-:4 =. !:ř-rí- :; =.,=ž.ž i'i=-. ž=a = i 7:ž: =ž==ž lii::: ž:=ž=,=, =:=zi = - =. j.Ě2 ':='niž=i4':= :ra =- -:1:1 =1= == -', = = = ,'=7 =-2 - -:: a 1' ' .: ==. >: .. : = ]= = ;: ? ži,== .;E : = ==: :=-,=== . !:1== 11=z; ; : 11z t=':,iž=.== = : =i :;'2 :ž='= =? zi = i: ; * 2,:i7+š =;=.- i Ť ž4 : i= ?E = Et=s; ř É€ E i á: HE * i € E ÉšE Ť g= E 5.:-ž=,, : B E řP *aÉ = ř€E $fr-UE;š e:= E?E E.qE = 3t;; -Ěč g í;"q€.Ei E =E !iEs ;E E;:ň€ĚEšE ĚŤ eřE fi :c E Pn: l : E€-.E,p p gE E EE€EŤ.=ŤsŘ E apu* E ř*E BÉĚF€ EěEř€ q€ EEi E3! EERE gEŠ:Eť E Ě ; g t c E [ á g PE s: =. n 3 3 E š6Ě € E i a*.! = o a"r E č€? Ež=E 3>šE }EE 8 $3 g 9EůE;R.sE ; ď;E
|
|
| |
guest ::
