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Sjögren's syndrome - anamnestic study
Rosecká, Michaela ; Jílek, Petr (advisor) ; Janďourek, Ondřej (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Michaela Rosecká Supervisor: PharmDr. Petr Jílek, CSc. Title of Bachelor thesis: Sjögren's syndrome - anamnestic study Backround: The aim of this work was to get acquainted with the problems of this serious illness and the comparison of the quality of life diagnosed in the Czech Republic and abroad. Main findings: Sjögren's syndrome is an autoimmune disease with exocrine gland disorders. The pathogenesis of this disease is still unclear, but knowledge of the involvement of various cells and the pathways leading to autoimmunity is increasing. The most common symptom is dryness of the eyes and mouth, but patients often have problems with musculoskeletal or systemic damage of the body. Sjögren's syndrome brings an increased risk of developing lymphoproliferative disease. Conclusions: Attempts to create new diagnostic criteria indicate the difficulty and great interest in understanding this disease. Accurate diagnosis should be performed by laboratory diagnostics in combination with appropriate tests. Still, it is a problem to identify this disease safely and Sjögren's syndrome remains an unknown and underestimated autoimmune disease.
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Streptococci of the anginosus group (SAG) - analysis of data associated with positive findings
Brieslingerová, Lenka ; Voxová, Barbora (advisor) ; Janďourek, Ondřej (referee)
Lenka Brieslingerová Streptococci of the anginosus group (SAG) - analysis of data associated with positive findings Bachelor thesis Charles University in Prague, Faculty of Pharmacy, Hradec Králové Medical laboratory technician Aim: The aim of the work is to summarize the current knowledge about the group of streptococci anginosus and the processing of data concerning patiens with a positive finding of these streptococci in the sample. The data was provided by the microbiological laboratory of FN Olomouc. Methods: Graphical processing of data on patients with positive SAG findings. Results: The mean age of patients identified with SAG was 50.2 years. Occurrence was more common in males than females. The most represented species in this work was S. anginosus (34%), followed by S. constellatus (34%), and S. intermedius (13%). The sample was most often derived from an infectious deposit, other abundant collection sites were the urogenital and respiratory tract. Each species is associated with a different type of infection. S. anginosus was the major patogen in urogenital system infections as well as in intraabdominal infections. S. constellatus was involved primarily in respiratory infections, most commonly in haemocultures. S. intermedius is usually found in abscesses and in this study, its...
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In vitro screening of novel potentially active antibacterial compounds
Davidová, Lucie ; Janďourek, Ondřej (advisor) ; Konečná, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Specialist in Laboratory Methods Candidate: Bc. Lucie Davidová Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of novel potentially active antibacterial compounds. The diploma thesis deals with the antibiotics policy and is focused on resistance and mechanism of action of the antibiotics. A great attention is also paid to novel substances testing in terms of their impact on the selected bacteria. Theoretical part of thesis describes the system of antibiotic policy including its structure and organization in the Czech Republic. It also briefly summarizes this issue globally as well as individual organizations involved in the organization of the antibiotic policy. The theoretical part is also concerned with the history, classification, effects and significance of the antibiotics. A particular attention is paid to the chapter describing the macromolecular mechanism of action of 2 selected groups of substances (antibiotics) on bacteria, including physiological functions of some bacterial structures. And, last but not least, the theoretical part summarizes principles of bacterial resistance including its origin and development. The diploma thesis...
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Detection of Sap2 in the secretome of Candida albicans cell wall and secretory mutants
Kollárová, Nikola ; Janďourek, Ondřej (advisor) ; Konečná, Klára (referee)
Candidate: Nikola Kollárová Title of diploma thesis: Detection of Sap2 in the secretome of Candida albicans cell wall and secretory mutants Charles University, Faculty of Pharmacy in Hradec Králové, Department of Biological and Medicinal Sciences Complutense University of Madrid, Faculty of Pharmacy, Department of Microbiology II Study program: Pharmacy Backgound: The aim of this diploma thesis was to search for C. albicans proteins involved in the secretion of the secreted aspartyl proteinase 2 enzyme (Sap2) evaluating the ability to degrade BSA (bovine serum albumin) as a source of nitrogen in several cell wall and secretory mutants of C. albicans. The work was carried out at the Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid. Methods: The supernatant samples of several Candida albicans mutants were tested by SDS-PAGE electrophoresis and stained. Bands corresponding to BSA were observed and compared to controls. The other method was counted with 96-well plate. Results: The correlation between optical density and degradation of BSA was observed. Some mutants with disability to degrade BSA were found in a pilot screening of the ability to degrade BSA using 96-well plate method. That fact was confirmed by SDS-PAGE electrophoresis. C. albicans mutants showing...
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The recommended vaccination of the adult population in the Czech Republic
Nová, Petra ; Rathouská, Jana (advisor) ; Janďourek, Ondřej (referee)
Author: Petra Nová, DiS. Title: The recommended vaccination of the adult population in the Czech Republic Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Králové Field of study: Medical Laboratory Technician Background: The aim is to refer the possibilities of recommended vaccines for adults and focus on the currently available vaccines on the Czech market. The partial aim of the work is to describe the importance of vaccination as such and partly to disprove the opinions of its uselessness. At the end, the work deals with data processing and evaluation of vaccines dispensing in two selected pharmacies. Methods: Statistical evaluation of vaccines dispensing in two private pharmacies in Jicin in 2014 - 2016. Evaluation includes the types of dispensed vaccines, gender and the age categories for which the individual vaccines are determined. Results: Concerning the dispensed vaccines, there were mainly vaccines against influenza, tick-borne encephalitis and viral hepatitis A. The most commonly prescribed vaccines were against the flu. In 2015, September-December period was recorded the largest number of these dispensed vaccines. Conversely, in 2016 there was the lowest interest in this vaccination. Persons between 45 - 60 years were the most represented age category for the flu...
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Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method
Švirlochová, Veronika ; Vejsová, Marcela (advisor) ; Janďourek, Ondřej (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Veronika Švirlochová Supervisor: Mgr. Marcela Vejsová, Ph.D. Diploma thesis title: Evaluation of activity of potentional antibacterial substances through the use of microdilution broth method Background The aim of this diploma thesis was to evaluate antibacterial activity of fifty-two newly synthetized substances. Methods Substances were tested through the use of microdilution broth method with eleven different concentrations. There were used eight bacterial strains and there were also used agents of nosocomial infections. Results Tested substancies were divided into seven groups by their chemical structure. The most effective group were sulphonamides. There were two groups with mininal or zero antibacterial activity. Conclusion The results showed that the most sensitive strain was Staphylococcus epidermidis and the least sensitive strain was Pseudomonas aeruginosa. There was also consideration about the presence of different substituents in different groups and their influence of antibacterial activity.
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Detection of Sap2 in the secretome of Candida albicans cell wall and secretory mutants
Kollárová, Nikola ; Janďourek, Ondřej (advisor) ; Konečná, Klára (referee)
Candidate: Nikola Kollárová Title of diploma thesis: Detection of Sap2 in the secretome of Candida albicans cell wall and secretory mutants Charles University, Faculty of Pharmacy in Hradec Králové, Department of Biological and Medicinal Sciences Complutense University of Madrid, Faculty of Pharmacy, Department of Microbiology II Study program: Pharmacy Backgound: The aim of this diploma thesis was to search for C. albicans proteins involved in the secretion of the secreted aspartyl proteinase 2 enzyme (Sap2) evaluating the ability to degrade BSA (bovine serum albumin) as a source of nitrogen in several cell wall and secretory mutants of C. albicans. The work was carried out at the Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid. Methods: The supernatant samples of several Candida albicans mutants were tested by SDS-PAGE electrophoresis and stained. Bands corresponding to BSA were observed and compared to controls. The other method was counted with 96-well plate. Results: The correlation between optical density and degradation of BSA was observed. Some mutants with disability to degrade BSA were found in a pilot screening of the ability to degrade BSA using 96-well plate method. That fact was confirmed by SDS-PAGE electrophoresis. C. albicans mutants showing...
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Derivatives of pyrazine as potential antituberculars (preparation and study of biological properties)
Janďourek, Ondřej ; Doležal, Martin (advisor) ; Farsa, Oldřich (referee) ; Musílek, Kamil (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical chemistry and Pharmaceutical analysis Candidate Mgr. Ondřej Janďourek Supervisor Prof. PharmDr. Martin Doležal, Ph.D. Consultant PharmDr. Jan Zitko, Ph.D. Title of Doctoral Thesis Derivatives of pyrazine as potential antituberculars (preparation and study of biological properties) This work is focused on pyrazine derivatives with the structural relationship to pyrazinamide (PZA) and with the potential antitubercular effect. In the introduction, there are summarized theoretical findings about tuberculosis (TBC), its epidemiology and primarily the development of resistance to current pharmacotherapy. Brief overview of known antituberculars, novel compounds and auspicious molecules in clinical trials is outlined. PZA, which is counted among the first line antitubercular agents, is widely described due to its cardinal role in this thesis. PZA possible mechanisms of action are described together with its pharmacological profile. Finally, brief summary of already prepared derivatives of PZA is stated. Practical part of this thesis is focused on synthesis of three starting compounds (5-chloro-6-methylpyrazine-2,3-dicarbonitrile; 3-chloropyrazine-2-carboxamide; N-benzyl-3-chloropyrazine-2-carboxamide) that were...
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Pyrazine derivatives as potential drugs VII.
Janďourek, Ondřej ; Doležal, Martin (advisor) ; Kopecký, Kamil (referee)
Pyrazin derivatives as potential drugs VII. Ondřej Janďourek Tuberculosis is one of the biggest health problems in the world. The number of new cases is rising every day, especially in less developed countries. The other problem is drug resistant tuberculosis which is occurring more and more frequently. Consequently these circumstances lead to one solution - to find new compounds to treat this greasy disease. This work deals with synthesis of undocumented compounds in literature. The starting compound was 5-chloro-6- methylpyrazine-2,3-dicarbonitrile. The compounds were developed by aminodehalogenational reaction by substituted anilines. Final compounds were characterized by molecular weight, melting point, TLC, logP, elemental analysis, 1 H and 13 C NMR and IR. Compounds were tested in vitro testing on their biological activity - antimycobacterial, antifungal and antibacterial.
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Derivatives of pyrazine as potential antituberculars (preparation and study of biological properties)
Janďourek, Ondřej ; Doležal, Martin (advisor) ; Farsa, Oldřich (referee) ; Musílek, Kamil (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical chemistry and Pharmaceutical analysis Candidate Mgr. Ondřej Janďourek Supervisor Prof. PharmDr. Martin Doležal, Ph.D. Consultant PharmDr. Jan Zitko, Ph.D. Title of Doctoral Thesis Derivatives of pyrazine as potential antituberculars (preparation and study of biological properties) This work is focused on pyrazine derivatives with the structural relationship to pyrazinamide (PZA) and with the potential antitubercular effect. In the introduction, there are summarized theoretical findings about tuberculosis (TBC), its epidemiology and primarily the development of resistance to current pharmacotherapy. Brief overview of known antituberculars, novel compounds and auspicious molecules in clinical trials is outlined. PZA, which is counted among the first line antitubercular agents, is widely described due to its cardinal role in this thesis. PZA possible mechanisms of action are described together with its pharmacological profile. Finally, brief summary of already prepared derivatives of PZA is stated. Practical part of this thesis is focused on synthesis of three starting compounds (5-chloro-6-methylpyrazine-2,3-dicarbonitrile; 3-chloropyrazine-2-carboxamide; N-benzyl-3-chloropyrazine-2-carboxamide) that were...
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