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Effectors of RAB GTPases and their role in plant secretion
Růžičková, Martina ; Hála, Michal (advisor) ; Motyka, Václav (referee) ; Klíma, Petr (referee)
Rab GTPases are small signaling molecules that play an important role in vesicle trafficking in eukaryotic cells. Correct signaling through small GTPases allows orchestration of vesicle transport among cellular organelles and also to the cell wall providing cell wall material for cell growth and elongation. Engagement of Rab GTPases in the regulation of endomembrane trafficking is one of the evolutionary conserved aspects of secretion regulation. The network of Rab GTPases interaction includes also various downstream effectors. One of them is the exocyst complex involved in vesicle docking at the plasma membrane. It is a complex composed of eight different subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84). Exocyst was discovered as Sec4p Rab GTPase effector in yeast and also data from animal models describe the Sec15 exocyst subunit as the Rab-interacting partner, but data from plants are missing. On the other hand, numerous studies identified exocyst role in tip growth of pollen tube and root hairs, seed coat formation, cell plate and cell wall formation, hypocotyl elongation, and importantly also PIN auxin efflux carriers recycling and polar auxin transport. There are two paralogues of SEC15 in the Arabidopsis genome, SEC15a and SEC15b, the previous one already shown to be...
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Closer analysis of gene trap line MGT180 and its candidate genes
Šnajdrová, Tereza ; Soukup, Aleš (advisor) ; Klíma, Petr (referee)
Root branching allows plants to explore rhizosphere, to gain efficiently water, mineral nutrients or enter in various biotic interactions. Initiation of lateral root formation is localized to pericycle cells, flanking the xylem poles of diarch vascular bundle in Arabidopsis thaliana. Right in these pericycle cells, there is the expression pattern of the gene trap line MGT180. In this theseis , I have provided the evidence that the expression pattern of MGT180 is related with AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED18 (AHL18; At3g60870). AHL18 belongs to a gene family of 29 transcriptional factors of Arabidopsis. AHL18 has not been functionally characterized yet, the analysis of singlemutant ahl18 and some others revealed no significant phenotype. However, one of doublemutants, E15, showed a significant phenotype. This phenotype was evident mainly in the aboveground part of plants, and was not corresponding to any phenotype of AHL mutation described so far. There is a known redundancy among some AHL genes, confirmed by crossing of ahl18 and ahl28 leading to E15 plant. Translation phusion AHL18-mRUBY and AHL22-mRUBY under native promotors should reveal, where these two related proteins act, and if they fiction in autonomous manner or not. Key words Arabidopsis thaliana, lateral root, AHL, pericycle
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Transformations of work localization in the Prague metropolitan region
Klíma, Petr ; Svoboda, Peter (advisor) ; Hána, David (referee)
This bachelor thesis deals with transformations of work localization in the Prague metropolitan region in the period from 1991 to 2011. The aim of presented study is to analyze the transformation of work localization in context with duality of core and periphery and to determine whether the work localization is affected by highway localization in hinterland and underground localization in core of metropolitan region. For the purpose of analysis I used data on work commuting from 1991, 2001 and 2011 censuses. The first part is devoted to the theoretical bases of work localization and to description of different conditions and processes that influenced work localization before and after year 1989. This section significantly focuses on description and explanation of the process of commercial suburbanization, which causes changes in work localization in the Prague metropolitan region after 1989. The empirical part is divided into two sections. In the first section data for municipalities are analyzed and is devoted to duality of core and periphery and to transformations of work localization in hinterland of the Prague metropolitan region in context with localization of highways. In the second part of thesis, data for basic settlement units from year 2011 are analyzed and localization of work in core of...
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The role of ARPC2 in plant cells
Šlajcherová, Kateřina ; Schwarzerová, Kateřina (advisor) ; Klíma, Petr (referee)
ARPC2 protein localization in a plant cell Kateřina Šlajcherová 1 Abstract Actin cytoskeleton is an ubiquitous structure which plays numerous irreplacable roles. Actin nucleation is, beside formins, performed by ARP2/3 complex (actin-related protein), comprising of seven subunits (ARP2, 3, C1-C5) and activated by protein SCAR/WAVE complex. ARP2/3 complex is attached to the membrane and branches existing microfilaments, apart from nucleating them de novo. ARP2/3 mutants in most organisms show severe defects. However, plant mutants exhibit only mild phenotype, for example, Arabidopsis thaliana ARPC2 mutant (dis2-1) has deformed trichomes and leaf epidermal cells, but its viability is not impaired. The aim of the thesis is to map ARPC2 localization within the cell and broaden our understanding of ARP2/3 complex role in plant cell morphogenesis. Tobacco ARPC2 (NtArpC2) subunit was visualized in Arabidopsis plants, using the GFP fusion protein as well as imunofluorescence and anti-ARPC2 antibody. Experiments were undertaken to collocalize the subunit with actin and microtubular cytoskeleton, with mitochondrions, endosomes and other membrane organelles. The specimens were observed in confocal and TIRF microscope. The GFP-NtARPC2 protein shows as motile dots; their movement, but not their existence, is dependent...
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A small three phase induction motor
Klíma, Petr ; Cipín, Radoslav (referee) ; Mach, Martin (advisor)
The main object of this bachelor thesis is to determine the parameters of the induction motor with an output of 155 W. Three ways of obtaining the parameters were chosen. Analytical calculation according to available literature, calculation in RMxprt program and laboratory measurement of the motor. For a better comparison of analytical calculations, two literary sources were chosen according to which the calculation was performed. Individual calculations and values obtained by them are included in the thesis. A comparison of the results obtained by individual methods is performed.
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Architecture and design of department stores by Růžena Žertová
Borůvková, Zlata ; Novotná, Eva (advisor) ; Klíma, Petr (referee)
Design of Department Stores by Růžena Žertová. The objective of the bachelor thesis is to introduce buildings of department stores designed by Růžena Žertová, constructed in the 1960s to the 1970s. The projects by this architect markedly surpassed the standard of contemporary food-processing-industry buildings. This thesis focuses on her work in Košice, Ústí n. L., and Pardubice.
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Mechanizmus transportu cytokininů přes buněčnou membránu a jejich metabolizmus v buňkách tabákové suspenzní kultury BY-2
Klíma, Petr ; Zažímalová, Eva (advisor) ; Galuszka, Petr (referee) ; Procházka, Stanislav (referee)
MECHANISM OF CYTOKININ TRANSPORT ACROSS PLASMA MEMBRANE AND THEIR METABOLISM IN TOBACCO BY-2 CULTURED CELLS Mgr. Petr Klíma / Abstract of Ph.D. Thesis / Prague 2011 Cytokinins (CKs) are plant hormones that play a major role in a number of developmental processes in plants. Those include promotion of cell division, active growth and differentiation, and maintenance of sink-source relationships, as well as control of environmental stress responses. Native CKs are low-molecular derivatives of adenine which seem to act either as paracrine or as long-distance signals. Due to their numerous physiological effects, plants have to precisely control the occurrence of bioactive CK molecules on the levels of the whole plant, its organs, tissues as well as single cells. To achieve this, a concerted action of metabolism and transport processes is required. Studies of the kinetics of CK translocation across plasma membrane in BY-2 suspension-grown tobacco cells suggested the existence of energy-dependent, partially selective transport routes for CK bases and CK ribosides. HPLC analysis of the metabolites of accumulated CKs pointed at their fast degradation or metabolic conversion into physiologically inactive forms. The prevalent ways of inactivation were the degradation to adenine and phosphorylation or phosphoribosyl...
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The Importance of a Coaching for a Leadership
Klíma, Petr ; Horáková, Jana (advisor) ; Fiedler, Jiří (referee)
The theme of this bachelor thesis is the importance of coaching for a leadership. The theoretical part explains the basis of the problematics of management and leadership, and then thoroughly examines the topic of coaching: its essence, outline, advantages and disadvantages. Ascertained information is accompanied by several case studies from the business and entrepreneurial fields. These case studies were analysed and compared and contrasted to the basic principles of coaching. Towards the end of the theoretical part, the potential benefits of coaching are put into the context of three objects (employers, graduates, and the society) and respective challenges they are facing.
The main objective of the thesis is to identify and asses the influence of coaching in leadership. To accomplish this objective, structured interviews were done with several managers. These interviews were then evaluated on the basis of the theoretical part.
At the end of this thesis is a summary of influences of coaching, and the review of their importance for effective leadership.
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High-speed Condesing Steam Turbine
Klíma, Petr ; Trojan, Aleš (referee) ; Fiedler, Jan (advisor)
ith one controlled extraction and one uncontrolled extraction, calculation of the flow channel at all stage, design and calculation of the regulation valve and create connection diagram of steam turbine and air cooled condenser. At the beginning of this work is an overview of manufacturers of steam turbines and their unified products. Master thesis was developer with G-Team, a.s. as using calculations and the instructions given in the recommended literature with supporting CFD simulations to determine the loss coefficients and FEA simulations to determine the eigenfrequencies blades.
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