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Introduction of an invertebrate animal model, Galleria Galleria mellonellamellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics
Kubíček, Jiří ; Konečná, Klára (advisor) ; Němečková, Ivana (referee)
Charles university, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Author: Jiří Kubíček Supervisor: RNDr. Klára Konečná, Ph.D. Title of thesis: Introduction of an invertebrate animal model, Galleria mellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics. Background: The aim of this diploma thesis has been to present an invertebrate organism, Galleria mellonella (G. mellonella), Pyralidae, as an alternative, more economically advantegous and morally more acceptable model for in vivo studies of an antituberculotics (anti-TB) efficacy. In the experimental part of this work, attention was paid to the optimization of methodological steps, that should lead to a higher reproducibility and validity of the obtained data. The effect of the disaggregating supplement, tyloxapol, on the growth of Mycobacterium aurum (M. aurum) was evaluated. The growth of this microbial strain in the culture medium, Middlebrook 7H9, was also mapped to determine the exponential growth phase. In the second part of the experiments, G. mellonella larvae were infected with a M. aurum bacterial strain, followed by an administration of the selected, commercially available anti-TB. Methods: To map the exponential phase of M....
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Medical center in Prostějov
Kubíček, Jan ; Hobzová, Klára (referee) ; Benešová, Romana (advisor)
The aim of this master´s thesis is to elaborate the project documentation of a new-built medical facilty for building permit, in the cadaster area of Prostějov. The building has six medical offices with facilities, pharmacy with the production of drugs and X-ray equipment. The building is simply-shaped, with two aboveground floors. The vertical loadbearing structures are designed from sand-lime blocks. The building is insulated by a ETICS with ventilated facade with green facade. The horizontal loadbearing structures and the staircase are designed from reinforced cast-in-place prefabricated parts. The concept of flat roof is an extensive green roof. The building is heated by gas condensing boiler. The building uses photovoltaics to produce electricity for save non-renewable resources. The building is ventilated using mechanical ventilation. In the third part, an assessment of water management was performed. The project was processed in ArchiCAD and Lumion.
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Portrait art in the work of Egon Schiele.
KUBÍČEK, Jan
The bachelor's thesis Portrait Art in the Work of Egon Schiele is divided into two parts, theoretical and practical. The theoretical part describes the important stages of life and cultural environment that influenced Egon Schiele. It also focuses on his selected artistic work, with an emphasis on portrait and self-portrait. The practical part is based on the findings of the first part, follows the self-portrait of Egon Schiele and tries to get inspiration in his distinctive style and technique. The aim of the practical part is a cycle of the bachelor's thesis author's self-portraits , accompanied by sketch material.
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Customer protection in the provision of investment services
Kubíček, Jan ; Kotáb, Petr (advisor) ; Sejkora, Tomáš (referee)
Customer protection in the provision of investment services Approaches to supervision This diploma thesis investigates possibilities and opportunities in the field of financial regulation and supervision conducted by the Czech National Bank. Its focus is the protection of customer in the area of investment services. The thesis begins with the research question and the reasons why it was chosen. Then, basic overview of the financial law framework in the Czech Republic is provided. The thesis continues with general remarks on deterrence and persuasion as two distinctive extremes. Some conclusions form the literature are compiled. A number of experiences is distilled from papers and empirical studies, too. Sources are not only from the field of financial regulation and supervision, but also from other fields. These form the basic framework for closer investigation of several supervisory/regulatory approaches. Then pros and cons of several regulatory approaches are evaluated. These are: responsive regulation, smart regulation, principles-based regulation and anticipatory regulation. One part is dedicated to evaluating conclusions drawn from real experiences with implementation of yet another approach, cooperative regulation (which has some similar characteristics with the others) in the field of tax regulation...
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Introduction of an invertebrate animal model, Galleria Galleria mellonellamellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics
Kubíček, Jiří ; Konečná, Klára (advisor) ; Němečková, Ivana (referee)
Charles university, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Author: Jiří Kubíček Supervisor: RNDr. Klára Konečná, Ph.D. Title of thesis: Introduction of an invertebrate animal model, Galleria mellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics. Background: The aim of this diploma thesis has been to present an invertebrate organism, Galleria mellonella (G. mellonella), Pyralidae, as an alternative, more economically advantegous and morally more acceptable model for in vivo studies of an antituberculotics (anti-TB) efficacy. In the experimental part of this work, attention was paid to the optimization of methodological steps, that should lead to a higher reproducibility and validity of the obtained data. The effect of the disaggregating supplement, tyloxapol, on the growth of Mycobacterium aurum (M. aurum) was evaluated. The growth of this microbial strain in the culture medium, Middlebrook 7H9, was also mapped to determine the exponential growth phase. In the second part of the experiments, G. mellonella larvae were infected with a M. aurum bacterial strain, followed by an administration of the selected, commercially available anti-TB. Methods: To map the exponential phase of M....
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I, you, we and they: forms of patriotism in teaching materials of the Czech educational system and the Czech classrooms
Kubíček, Jan ; Lánský, Ondřej (advisor) ; Samek, Tomáš (referee)
This thesis focuses on the form of the discourse of nationalism in educational materials, specifically in selected history and civics textbooks for primary schools. The aim of the paper is not to cover all available educational materials in the Czech school environment, but to conduct a probe into textbooks from selected subjects from one publisher, which serves as a sample. There are subtle forms of nationalism that are part of public discourse, and the thesis will map these forms in the textbooks as manifestations of the discourse of nationalism, which seeks to shape national identity, among other things. Therefore, the work follows in these textbooks their language, the topics they choose, the ways in which they report on the topics and the other media they use (e.g. visual documents). The thesis examines the aforementioned elements of the textbooks through qualitative analysis, specifically critical discourse analysis. The main question that the thesis seeks to answer is which discursive means are used to form Czech national identity, and whether this identity also includes an ethnocentric view of the world. The thesis is divided into three main parts. The first chapter focuses on the key concepts needed for the research. The second chapter focuses on the research methodology. The theoretical...
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Use of torch swing for butt weld welding of stainless steel MAG method
Gago, Matúš ; Sigmund, Marian (referee) ; Kubíček, Jaroslav (advisor)
Diploma thesis of arc welding of stainless steels focused on the MAG method, with a literature study on the issue. The geometries of the butt weld with the preparation of the bevel "Y" and the thickness of materials that are often used on structures used in the energy and food industries (6, 8 and 12 mm) were selected. An experimental study of the effect of MAG welding was performed, with and without oscillation of the welding torch for the "Y" weld. Experimentally verified sample welds of individual types were supplemented by the evaluation of results with fillet welds and submerged arc welds for comparing the consequences of welding with high heat input. The materials used were theoretically evaluated, the possible resulting theoretical material structure was analytically calculated and the results were included in the Scheaffler and WRC 1992 diagram. The mutual weldability of the materials with the equivalent of Crom and Nickel was assessed. And evaluated macrostructure of weld patterns, developed tensile tests and hardness tests to assess the impact of mechanical properties. In the end, recommendations for practice and recommendations for the prevention of errors, which were observed on experimental samples, were set.
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The problems of welding stainless steel 1.4541
Šmarda, Vladimír ; Sigmund, Marian (referee) ; Kubíček, Jaroslav (advisor)
This thesis focuses on austenitic steel welding in the upper part of a steam generator at the nuclear power station Dukovany, and the suitable technology when replacing this part. Part of this thesis is dedicated to the progressive methods TIG, such as the use of hot wire or orbital welding of pipe parts. The experiment in this thesis project tests the optimal parameters setup when welding while using a modified TIG method; a motor-fed hot wire with a welding source from EWM manufacturer was used in this project. Number of NDT tests, along with destructive tests including metallographic tests, demonstrated and verified that the methods, the additional materials and the way the experiment was carried out were chosen correctly and proved effective. These tests also included the hardness of the welding point and TOO locations. The experiment assessment part summarizes the experiment execution, describes the results of the tests, and recommends further continuation in the process toward WPQR
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