|
| |
|
| |
|
|
Cartographic Works for Children and Youth: State of Art and Possibilities of GIS Utilization
Škardová, Marta ; Bláha, Jan Daniel (advisor) ; Hudeček, Tomáš (referee)
Abstract:: The aim of this paper is to present cartographic works for children and youth. The main object is the introduction to the problems of cartographic production for children and youth and the execution of value analysis in terms of cartography. The analysis focuses on the use of mapping language - character key and means of expresion, in this part is an efort to answer the question whether the cartographic works for children and youth are the real maps or rather an illustration. The assesment of selected product by their users - children continue the analasis. Another aim is to compare traditional methods of creating those works with the possibilities of modern computer cartography, three ways of making are demonstrated - by hand, using a graphic application and using cartographic application. Then the paper tries to assess the use of geographic and cartographic knowledge in products for children and youth, in this question the paper use knowledges gained in consultation with author from practise.
|
|
| |
|
| |
|
|
Influence of crystalloid and colloid solutions on blood coagulation using the rotational thromboelastometry (ROTEM) method
Binterová, Silvie ; Durila, Miroslav (advisor) ; Vaněk, Tomáš (referee) ; Bláha, Jan (referee)
Fluid resuscitation with crystalloid and colloid solutions is a common treatment in perioperative medicine. However, a variety of unbalanced or balanced solutions is used in clinical practice and there is still a vivid debate going on regarding the selection of optimal fluid with minimal negative effect on coagulation. The goal of the dissertation was to investigate the adverse effect of balanced crystalloids and colloids on whole blood coagulation measured by method of rotational thromboelastometry. In the first phase of the work we had assessed the adverse effect of balanced crystalloid, hydroxyethyl starch and gelatin after dilution of blood with the solution in vitro. Parametrs of EXTEM and FIBTEM tests were evaluated by using rotational thromboelastometry. In the second phase of the work we evaluated the negative effect of infusion solution after dilution in vivo. We had obtained blood samples from 30 patients during knee arthroscopy before and after administration of 500 ml of crystalloid or hydroxyethyl starch or gelatin. Parametrs of EXTEM and FIBTEM tests were evaluated by using rotational thromboelastometry.In compliance with the results of the dissertation, hydroxyethyl starch has the most obvious negative effect on clot formation followed by gelatin and finally by crystalloids. Based on...
|
|
| |
|
|
Assessment of mental maps in GIS
Kynčlová, Martina ; Hudeček, Tomáš (advisor) ; Bláha, Jan Daniel (referee)
This work deals with the possibilities of assessments of mental maps using GIS tools. The main goal is to create a collection of methods to evaluate positioning and relative accuracy of mental maps. The method principles are based on methods of GIS. The emphasis is on maximum versatility and automation the assessment procedure. For all proposed methods there are further discussed the advantages and disadvantages. First part of this work deals with the theme of mental maps, followed by a brief introduction to GIS and their use in the field mental maps. The main part is devoted to the description and practical examples of evaluation methods. At the conclusion there is a discussion of the proposed methods and the appropriateness of use GIS at this issue in general. This work was created under the project No. 26609 called "Mental Maps: Object and Device for Assessment" supported by the Grant Agency of Charles University.
|
|
|
Study of structure and interaction of human lymphocyte receptors
Bláha, Jan ; Vaněk, Ondřej (advisor) ; Šulc, Miroslav (referee) ; Obšil, Tomáš (referee)
Natural killer (NK) cells are an essential part of immune system, providing self-surveillance of virally infected, stress transformed or cancerous cells. NKR-P1 receptors and their ligands from clec2 gene family represent an alternate missing-self recognition system of NK cells based on interaction of highly related C-type lectin-like receptors. Human NKR-P1 has been described more than twenty years ago but still remains the sole human orthologue of this receptor family, particularly numerous in rodents. On binding to its cognate ligand LLT1, NKR-P1 can relay inhibitory or co-stimulatory signals. Although being interesting targets for their potential role in tumor immune evasion and autoimmunity, nature of their interaction is still unclear. To elucidate the architecture of their interaction, we developed a generally applicable method for recombinant expression of human NKR-P1 and LLT1 and their homologues based on transfection of HEK293S GnTI- cells. Further, we described a stabilizing mutation His176Cys, that enables for expression of highly stable and soluble LLT1. Finally, we have crystallized LLT1 and human NKR-P1 in different glycosylation states both as individuals and in complex. While both structures of LLT1 and NKR-P1 follow the classical C-type lectin-like superfamily fold, contrary to...
|
|
|
Preparation and study of human lymphocyte receptor LLT1
Bláha, Jan ; Vaněk, Ondřej (advisor) ; Konvalinka, Jan (referee)
Natural killer (NK) cells are an intensively studied part of immune system, possessing unique ability to recognize and induce death of tumor and virus-infected cells without prior antigen sensitization. Their function is regulated by a fine balance of signals induced by multiple activating and inhibitory cell surface receptors and their interaction with the ligands present on the target cell. Recent research in their C-type lectin-like receptors repertoire has shown that ligands of some of these previously orphan receptors lie within their own family, describing a lectin-lectin interaction. This is the case of human inhibitory receptor NKRP1 (gene KLRB1) and its ligand LLT1 (gene CLEC2D). Previous studies have shown that overproduction of LLT1 in cancer cells or lower production of NKRP1 in NK cells is connected to cancerous manifestations. This master's thesis shows a successful production of the extracellular part of LLT1 utilizing a mammalian expression system based on transient transfection of modified human embryonic kidney (HEK) cell lines. It was found that the five cystein residues contained within the lectin domain of LLT1 tend to cause misfolding and formation of aggregates. Stabilization of the domain was achieved by restoration of the sixth cystein residue at the evolutionary conserved...
|
guest ::
