National Repository of Grey Literature 22 records found  beginprevious13 - 22  jump to record: Search took 0.00 seconds. 
Production of sample of testing siderophore preparation
Palyzová, Andrea ; Kyslík, Pavel
Description of batch culture process of fur mutant of wild strain Pseudomonas aeruginosa PAO1 for siderophores production in continuously stirred bioreactor and preparation of lyophilized mixed siderophores (pyoverdin and pyochelin) sample.
Transfer of technology for production of AEH from Sphingomonas sp
Kyslíková, Eva ; Palyzová, Andrea ; Marešová, Helena ; Štěpánek, Václav ; Zahradník, Jiří ; Bečka, Stanislav ; Dobišová, Marie ; Kyslík, Pavel
Research report on construction of a recombinant production microorganism, the way of its cultivation in stirred bioreactor, overproduction and isolation of AEH and stabilization of AEH as cross linked enzyme agregate
Development of recombinant Pichia pastoris strain expressing synthetic gene of npga by genetic cloning
Marešová, Helena ; Maršálek, Jaroslav ; Bečka, Stanislav ; Kyslík, Pavel
Cloning of synthetic npga gene into Pichia pastoris. Development of high expression system for penicillin acylase in a stirred bioreactor. Isolation and characterization of the enzyme.
Simeprevir
Bečka, Stanislav ; Babiak, Petr ; Kyslík, Pavel
Screening of KRED enzymes capable of enantioselective hydroxylation of ketones.
Biotransformation of olefine
Marešová, Helena ; Kyslíková, Eva ; Kyslík, Pavel
Isolation and screening of soil microorganisms capable of biotransformation of olefines.
Purifikace a charakterizace penicilin-G-acylasy exprimované v Escherichia coli a Pichia pastoris
Marková, Zdenka ; Marešová, Helena ; Kyslík, Pavel
The pga gene coding for penicillin G acylase from strain E. coli RE3 was cloned on pPICZA plasmid and expressed in the yeast Pichia pastoris X33. The enzyme was purified from both strains and further characterized
Správné a chybné skládání chimérní pyranosa-2-oxidasy exprimované v E. coli
Marešová, Helena ; Kyslík, Pavel
Recombinant and chimeric pyranose 2-oxidases from different parental strains (Trametes ochracea and pubescens) were expressed in E. coli and formation of inclusion bodies were followed. The two, closely-related enzymes differ in folding into an active form under identical growth conditions

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