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Molecular Genetic Applications in Archeological and Anthropological Context
Urbanová, Markéta ; Černý, Viktor (advisor) ; Brdička, Radim (referee) ; Brůžek, Jaroslav (referee)
6 Coxcr-usroN The aim of our studywas to establisha laboratoryprotocolfor isolationand analysisof aDNA. By studying three different skeletal collections answersabout sex, kinship and presenceof pathogenicbacteriawere searched. During the course of this thesisnew protocols for aDNA study were developedand continuously optimized'We wereableto evaluateinformationaboutsexof 37 subadultindividuals(.l8'7%)from Mikulčice settlementcemetery(9thcentury)andto confirmpÍesenceof Mycobacteriumlepraeon 2 skeletonsfromŽatec cemetery(l3th century).Subadult skeletonsexaminationbrought interestingresults of extremelyhigher presenceof maleindividuals. Unfortunately,notall aimswerefulťrlled.By establishedprotocolfor aDNA kinshipanalysisit wasnot possibleto retrievesufficientinťormationfor explanationof familial relationshipof individualsfrom thesame doubleburialsite. All acquiredexperienceand establishedprotocols are very valuable and in fururewe supposetheir full utilizationandimorovementin thefield of kinshioanalvsisof aDNA molecule. 28
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Molecular Genetic Applications in Archaeological and Anthropological Context
Urbanová, Markéta
The aim of our study was to establish a laboratory protocol for isolation and analysis of aDNA (ancient DNA). By studying three different human skeletal collections answers about sex, kinship and presence of pathogenic bacteria were searched. During the course of this thesis new protocols for aDNA study were developed and continuously optimized. We were able to evaluate information about sex of 37 subadult individuals (78.7 %) from Mikulčice settlement cemetery (9th century) and to confirm presence of Mycobacterium leprae on 2 skeletons from Žatec cemetery (13th century). Subadult skeletons examination brought interesting results of extremely higher presence of male individuals. Confirmation of leprosis on skeletal findings provided very useful historical information about this disease presence on our Czech lands. Unfortunately, not all aims were fulfilled. By established protocol for aDNA kinship analysis it was not possible to retrieve sufficient information for explanation of familial relationship of individuals from the same double burial site. All acquired experience and established protocols are very valuable and in future we suppose their full utilization. At the same time, this study will be surely valuable for other archaeological and anthropological institutions.
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