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Preparation of analogues of MRI contrast agents and study of their internalization on cell cultures
Krijt, Matyáš ; Poljaková, Jitka (advisor) ; Martínková, Eva (referee)
Magnetic resonance imaging (MRI) is a commonly used non-invasive method to examine internal organs and soft tissues. In order to increase signal intensity and improve specificity contrast agents (CAs) have been developed, which affect T1 or T2 relaxation times. T1-contrast agents are mostly based on Gd(III) complexes. The aim of this thesis was to prepare analogues to commercial Gd(III)-CAs by changing the central atom for europium and use europium fluorescence properties for observing CAs' cell-distribution. However, due to strong cell auto-fluoresce the fluorescence of complexes did not provide convincing images reporting their biochemical fate. The toxicity of prepared complexes was tested on adherent cell cultures and evaluated by Fluorescence-Activated Cell Sorter (FACS). Internalization of the CAs' analogues was confirmed also by elemental analysis (ICP-MS). (In Czech)
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Monitoring of Selected Socially Excluded Locations in the Town of Plzeň
MARTÍNKOVÁ, Eva
The theoretical part of this thesis defines the process of social exclusion and its elements - spatial, economic, cultural, social, political and symbolic. These interconnected elements give rise to crucial areas that are substantially affected by social exclusions - living conditions, employment or ways of making a living, education and medical condition. The following chapters describe the initial situation in the Plzeň locations in 2006 and the situations in the mentioned areas in relation to excluded individuals. The objective of the thesis presented is to describe the development in seven socially excluded locations in Plzeň including their current situation. A qualitative survey was carried out addressing particularly workers in self-administration and non-governmental non-profit organisations, who come in contact with individuals from socially excluded locations. A method of document analysis by means of content analysis of the data was used along with a questioning method via a dialogue. The situation of socially excluded locations in Plzeň has significantly changed in the last five years, as the location labelled as the most problematic one by all informers perished at the end of 2009 and another location suffered changes as a result of the sale of the property. Maintaining or on the other hand cessation of the described localities considerably depend on the town´s housing policy, since the town of Plzeň owns the houses in all remaining locations. Owing to the increasing indebtedness of the population and growing problems in the housing or employment areas, there is a threat of an origination of new socially excluded locations. This thesis contributed to the evaluation of the development in the situation of socially excluded locations in Plzeň, which can give a base to implementing direct measures resulting in joining the inhabitants of these locations as well as in creating preventive measures.
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Consulting yesterday, today and tomorrow or else Work Specifics of the Centre for Refugees and Migrants in České Budějovice
MARTÍNKOVÁ, Eva
The Centre for Refugees and Migrants in České Budějovice has been established within the České Budějovice Diocesan Charity project and currently it has been in operation for fifteen years. My work deals with development of this Centre {--} I have been focused on how its activities, clients, used and provided services developed during the individual years of its existence. I monitor development of the Centre for Refugees and Migrants Centre project, focused on activities of former and current staff, considering specifics of the Centre. One of the specifics of the Centre for Refugees and Migrants in České Budějovice is employee fluctuation. Service framework has been established during the first years of Centre projects´ existence {--} however considering change in type of clients, characters of problems varies as well as scope of utilization of individual services and demands on the staff.
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