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Superresulution of photography using deep neural network
Holub, Jiří ; Přinosil, Jiří (referee) ; Burget, Radim (advisor)
This diploma thesis deals with image super-resolution with conservation of good quality. Firstly, there are described state of the art methods dealing with this problem, as well as principles of neural networks with focus on convolutional ones. Finally, there is described a few models of convolutional neural network for image super-resolution to double size, which have been trained, tested and compared on newly created database with pictures of people.
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Audio amplifier with PWM modulation for laboratory measurements
Holub, Josef ; Petržela, Jiří (referee) ; Kratochvíl, Tomáš (advisor)
This paper deals with the theoretical analysis of the essential parts of amplifier operating in Class D. It describes the principles and the relationship between the individual blocks. The second part deals with the design of the block connection of a switched amplifier for a laboratory device. The design part contains calculations, justification of the selection of individual components and integrated circuits and schematic connection within the individual blocks. Some blocks are simulated using PSpice and FilterFree. The work also deals with the structural design of the jig and the creation of a 3D model. The annex is contained overall diagram of an amplifier and a complete reference material for production.
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Optimization of extraction of metabolites produced by selected strains of microalgae and carotenogenic yeast.
Obračaj, Jan ; Holub, Jiří (referee) ; Szotkowski, Martin (advisor)
This bachelor thesis is focused on the production of microbial biomass using selected strains of cyanobacteria and carotenogenic yeasts. Subsequent preparation of extracts and study of their stability under different temperature conditions with the addition of the antioxidant tocopherol. In the experiment, 2 series of extracts with hexane and ethanol were prepared. The theoretical part of the work describes the studied metabolites carotenoids, chlorophylls, ubiquinone, ergosterol and microbial lipids. Furthermore, the used strains of cyanobacteria and carotenogenic yeasts are described here. Finally, methods for extraction and analysis of metabolites are described. The experimental part describes the used cultivation techniques, preparation of microbial extracts and used analytical methods. The results part of the work then contains a summary of the results of long-term stability tests of extracts. The results of the work clearly confirm the temperature dependence of the stability of individual metabolites. This phenomenon was most evident in chlorophyll and carotenoid pigments. Hexane appears to be the best solvent in terms of the stability of the substances used. The results of this work provide important information in terms of the economics of storage of these valuable substances usable in the food, pharmaceutical or feed industries.
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Application of chemical and physical stresses in the late phase of growth to selected strains of microorganisms
Langer, Marek ; Holub, Jiří (referee) ; Szotkowski, Martin (advisor)
A wide spectrum of microorganisms is used in the biotechnological industry for production of meaningful metabolites; including carotenoids and chlorofyles with antioxidant effects, fatty acids, ubiquinone and ergosterol. Ways to increase yield of metabolites to maximum capacity are being researched to increase efficiency and economical sustainability in industry. One of the ways is the application of chemical stress factors on microorganisms as is described in this thesis. In the theoritical part certain microorganisms, their important intracellular metabolites and their metabolism are established. The last subchapter covers the usage of stress factors in industry. In the experimental part each microorganism was subjected to various concentrations of sodium chloride, cobalt(II) sulfate and iron(III) chloride. Significant increase of lipid production was registered after an application of sodium chloride on yeast strain Rhodosporium toruloides. An increase in production of torularhodin in Sporidiobolus pararoseus occured after application of iron salt. After an application of sodium chloride the growth of torularhodin in strains Cystofilobasidium macerans a Rhodosporidium toruloides was significant. Sodium chloride was also a suitable stress factor for lycopene formation in Rhodotorula mucilaginosa. The most meaningful stress factor of algae was cobalt(II) sulfate which increased metabolite production in Scenedesmus obliquus. Cyanobacteria Anabaena torulosa also attained a significant production in the presence of sodium chloride.
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Control of lighting with DALI protocol in the KNX bus system
Holub, Jiří ; Škoda, Jan (referee) ; Bátora, Branislav (advisor)
The thesis is focused on control of lighting with DALI protocol in the KNX bus system. There are briefly described different types of bus systems. The work partly describes the digital bus system DALI for lighting operating. It analyses the ETS3 software environments used for programming the KNX installations. Within the framework of the practical part, two laboratory panels equipped with ABB Company´s elements were created. For these panels, four laboratory manuals were drawn up. Each manual includes the task, the instructions, the brief description, the connection diagram and the procedure. The manuals describe single ABB's elements such as the KNX light module, the KNX roller blind module, the DALI light module and the usage of LCD controller priOn to operate all of the modules used in the tasks.
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USB thermometer
Holub, Jiří ; Kubánek, David (referee) ; Lattenberg, Ivo (advisor)
This bachelor thesis deals with a problem of electrical measurement of temperature. It describes a various types of temperature sensors and processing data obtained from these. It describes in short the USB interface and its parameters. It also contains a design of hardware and software solution of thermometer using temperature sensor SMT 160, managed by microcontroller ATmega16 and communicating with PC via USB interface. Designed thermometer also offers possibility to save measured data to built-in memory and later load them from there. Measured data will be also displayed on LCD. Last part of these thesis is design of application for PC, which will serve for displaying measured data and adjustment of thermometer.
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Protection setting concept for given MV substation
Váhala, Václav ; Holub, Jiří (referee) ; Topolánek, David (advisor)
The thesis is focused on the protection concept for medium voltage substation. The first theoretical part of thesis describes given medium voltage substation. Substation consists of two incoming feeders and six outgoing feeders. Due to the size of the substation was selected one outgoing feeder which is the subject of description and protection concept. Next part of the thesis is a description of the protective functions used to protect the elements in the selected feeder. This part contains a detailed description of the function and setting options for protection. The main part of the thesis describes calculation of specific protection functions and consequently with secondary testing of protective functions. Tests were performed on protective functions in the digital protections REF615 and REM620. The final part of the thesis deals with the evaluation of the secondary test results and verification saturation of the specified current transformers.
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Testing of biological effect of glucans on cell lines in vitro
Šimková, Eva ; Holub, Jiří (referee) ; Němcová, Andrea (advisor)
The aim of this bachelor thesis is isolation and testing of -glucans from selected strains of yeast. In the theoretical part, characterisation of -glucans, microbial production of -glucans by yeast, determination of polysaccharides, exopolysaccharides and in vitro testing are described. Yeast have a cell wall made up of -glucans and -glucans are known for their healing properties. In the experimental part of this work deals with characterization of selected biomass yeasts in different forms and comparison of their effects and the amount of -glucans in each form. Stains of Metschnikowia pulcherrima, Cystofilobasidium infirmominiatum and Rhodotorula kratochvilovae have been selected for this thesis. Subsequently, the thesis deals with the isolation and testing of isolated -glucans from a selected lyophilised strain of yeast Rhodotorula kratochvilovae. The selected strain was isolated in 2 ways. The first method of isolation that was more friendly to conformation of -glucans due to the effect of ultrasonic homogenization of cells. The second method of isolation was less friendly to -glucans, using acid and base to homogenise them. Analyses of both extraction methods confirmed the presence of isolated -glucans, however it was found that the recovery of both methods was relatively low and requires further optimisation of the procedure. Overall, the better extraction procedure was the second method. A sample of this extract was further tested on the HaCaT cell line. The results of the pilot tests confirmed the cytotoxicity of the extract obtained by us.
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Modelling of the disperion properties of the atmosphere in vertical and slant direction
Holub, Josef ; Látal,, Jan (referee) ; Hudcová, Lucie (advisor)
This diploma thesis deals with the propagation of the optical beam through the Earth's atmosphere in the vertical and oblique directions. The work describes the properties of the Earth's atmosphere and parameters of atmosphere and its effects on the propagation of the optical beam, especially the optical dispersion. As part of the work, a model of optical beam propagation through the atmosphere was created in two versions, script and user-graphic. The model was created in the MATLAB environment and allows to draw the profile of the refractive index of the environment for the specified wavelength of radiation, it contains other graphical and numerical outputs.
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