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Study of liposomes by using of drop coating deposition Raman spectroscopy
Suchá, Alexandra ; Kočišová, Eva (advisor) ; Procházka, Marek (referee)
The method of drop coating deposition in Raman spectroscopy is based on the mea- surement of Raman spectra from a dried deposit of the studied sample, which is formed by drying a drop of a solution or suspension deposited on a solid hydrophobic surface. By drying the drop, the substance is pre-concentrated which achieves a higher detection sensitivity compared to the measurement of Raman spectra from a solution. In this work, we studied homogeneous and non-homogeneous suspensions of liposomes in different con- centrations from five types of single-component lipids and two natural lipid extracts. We deposited a drop of the suspension onto a commercially available hydrophobic substrate µ-RIMTM , measured the droplet static contact angle and characterized the formed depo- sits by their area and the width of their edge rings. We measured the Raman spectra from the ring and determined the ratio of the intensities of the selected spectral bands corre- sponding to the stretching vibrations of the group -CH2- (I2880/I2850), which characterize the order and phase of the lipid. The obtained results show that deposits from homogene- ous suspensions differ from inhomogeneous suspensions by the presence of clearly defined edge rings, a smaller area of the deposit and by a higher intensity of spectral bands in the Raman...
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Learning Progression-Based Assessment of Modern Genetics
Procházka, Marek ; Janštová, Vanda (advisor) ; Jáč, Martin (referee)
Molecular biology and genetics represent some of the most challenging biological topics for students, a correct understanding is also essential for the other chapters of biology and chemistry. The learning progression test is a tool that allows to determine the level of understanding of the given fields, which can serve as feedback that can be followed up in a targeted manner in further teaching. The aim of this work was to translate and subsequently validate the test of learning progression in molecular biology and genetics from the English original "Learning Progression-Based Assessment of Modern Genetics". Another goal of the thesis was to explain the possible influence of variables (e.g. participation in a biology seminar, science competitions or future study focus) on the overall score. Validation took place in several phases (checking the translation by experts and didactics, testing at schools and subsequent evaluation and analysis). Grammar school students completed the test online during biology class. They achieved a low level of understanding of heredity at the molecular level, further in the area of the influence of the environment on genetic information, the transfer of genetic information to offspring, and in general it is difficult for them to apply theoretical knowledge to specific...
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Design and implementation of a digitally signed report in the Apache JMeter
Procházka, Martin ; Člupek, Vlastimil (referee) ; Šeda, Pavel (advisor)
This thesis focuses on the development of a plugin for Apache JMeter to automatically generate report from the results of performance testing provided by other modules. In the theoretical part, it discusses the capabilities of Apache JMeter, stress testing in general, the structure of PDF document, the theory of digital signature and relevant cryptographic requirements. The practical part deals with the implementation of the report generator. First, it introduces the content of the report, then it discusses the principles of HTML and PDF report generation. Finally, the paper discusses the insertion of a digital signature into a PDF document.
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Reconstruction of a cinema building
Uhrinec, Marek ; Procházka, Martin (referee) ; Sobotka, Jindřich (advisor)
The diploma thesis is focused on a reconstruction of a cinema building in the town Gbely. It’s complete reconstruction of exterior and interior too. Original function, cinema, is change to sport and cultural centrum now. Object is consist of cafe, squash hall, climbing wall and sauna with rest room. Several thermal insulations are used to isolate object and satisfy a norm terms. The foundations are post insulated too. The interior is divided into functional spaces with Heluz system. New finishes in the object is created by brick paving, klinker, ceramic tiles and tiling. All windows and doors are replaced by new ones in the object. Sliding doors are used in interior for save space. The windows and doors are plastic between interior and exterior. All sanitations are replaced by new ones too. The reinforced concrete floor is used instead of lead bearing lintels. The wheelchair conditions are satisfied on ground floor.
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Temperature Profile in Reflow Soldering and Influence of Different PCBś and ComponentsThermal Capacities
Procházka, Martin ; Špinka, Jiří (referee) ; Starý, Jiří (advisor)
This thesis mainly deals with the prediction of temperature on the components and the PCB during reflow soldering. The theoretical part describes the particular solder reflow process, types of heat transfer and temperature profiles. The practical part is divided into forecasting temperatures if the conveyor is stopped and the temperature predictions when the conveyor is in motion. In both parts of the measured temperature is compared with the predicted temperatures, which show the success rate of prediction. The last part of this work is part of the simulation, which helps in proper understanding of the issues discussed.
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Detached House with an estab
Martiš, Patrik ; Procházka, Martin (referee) ; Čuprová, Danuše (advisor)
The aim of my bachelor´s work was to create a project documentation for a new family house with facilities. As a part of facilities I have chosen room for fitness activities. The family house is situated in the land register of Brno. It is a two storey house that is partially with basement. The house is designed into the shape of the letter L and it is suitable for a family made of 4-6 members. The object is based on the concrete boards and it is made of ceramic prophylactic bricks. It is covered by flat roof with Attica. I designed the project with emphasis on disposal orientation according to the cardinal points and constructed arrangements of the building. During the process I tried to use modern methods, common material and I tried to separate space for the family and space for fitness activities.
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Meatpacking plant
Majtán, Marián ; Procházka, Martin (referee) ; Sobotka, Jindřich (advisor)
The diploma thesis is focused on project documentation of Meat packing plant. Along with meat processing the documentation solve question of accomodation for employees. Building consists of two main sections: The processing – it is structured under requierements of meat packing industry. Second one is residential part set in 2nd, 3rd and 4th floor. Main inspiration for design are meat packing plants in Scandinavian countries, where is often need to provide accomodation to employess because of disadvantageous geographic locality. Building is built of Heluz building system, perimeter walls consists of insulation filled blocks. Building is without basement and it is based on footings of plaint. The roof is flat, partially designed as extensive green roof. Isover system is used to thermal insulation. A part of object is also asphalted plain for transport goods to and from the meat packing plant.
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Five-cylinder in-line diesel engine with viscous type damper
Procházka, Miloslav ; Novotný, Pavel (referee) ; Píštěk, Václav (advisor)
The aim of this diploma work is to design moving parts of the five cylinder inline diesel engine with given parameters, then to balance centrifugal forces and moments and design viscous torsional vibrations damper and calculate forced oscilations without the damper as well as with the damper. The designed parts are put to stress analyse by finite elements method. My design is based on similar construction of four cylinder engine Zetor.
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Detached family house, Hrabová
Pěničková, Klára ; Procházka, Martin (referee) ; Müller, Jan (advisor)
This bachelor´s thesis presents the design of a new house focussing on the project documentation for the building realization. The house comprised of one storey and a basement is designed for a family of four. The building is located in a slope terrain in the village Hrabová u Dubicka. Foundation structures are realized with plain concrete continuous footing. Vertical supportive constructions are designed from lost formwork concrete panels and the Liapor blocks. Ceiling constructions are realized with supported reinforced concrete slabs. The building is flat roof. The project documentation is processed with the help of the ArchCAD computer programme.
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