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	Decomposition of cellulose and hemicelluloses by wood-rotting fungi and effect of heavy metals Valášková, V. ; Merhautová, Věra ; Gabriel, Jiří ; Baldrian, Petr Degradation of cellulose and hemicelluloses by brown-rot fungi is significantly affected by heavy metals. The activity of cellulases is increased in the presence of zinc. Detailed record
	Směrovaná polymerní léčiva Říhová, Blanka Unwanted side effect of anticancer treatment could be eliminated by affinity therapy. It is necessary to find targeting system which deliver drugs specifically to tumor site. Polymeric conjugates based on N-(2-hydroxypropyl)methacrylamide are new generation polymeric anti-cancer drugs with enhanced cytostatic activity, but minimalized unwanted side effects. Detailed record
	Cyclické peptidy a depsipeptidy jako markery houbových infekcí Jegorov, A. ; Sulc, M. ; Ulrych, A. ; Navrátil, P. ; Curn, V. ; Kratochvíl, B. ; Havlíček, Vladimír Fungi produce cyclic peptides and depsipeptides by multifunctional synthetases. Detailed record
	Accumulation of metals in fruiting bodies of Fomes fomentarius Gabriel, Jiří ; Baldrian, Petr ; Kofroňová, Olga ; Benada, Oldřich Fruit body of Fomes fomentarius was used for detailed study of metal distribution within fungus and wood. Higher content of Mn was found in the core. Detailed record
	Accumulationn of heavy metals by wood-rotting fungi Gabriel, Jiří This work summarizes data on heavy metal content in wood-rotting fungi. Their effect on the physiology of fungi is also discussed. Detailed record
	Konstrukce neoznačených mutantů v genech kódujících Ser/Thr proteinkinasy a fosfoproteinfosfatasu Pseudomonas aeruginosa a analýza jejich vlastností Nedvědová, Jana ; Lněnička, Petr ; Hercík, Kamil ; Branny, Pavel To investigate the importance of individual protein kinases and phosphatases in P. aeruginosa physiology we prepared the single and double mutants in the coresponding genes. Single mutants in protein kinase genes stk1, ppkB and protein phosphatase gene stp1 were prepared by the method of gene replacement with subsequent excision of antibiotic cassettes using Flp-FRT recombination system. Two-dimensional gel electrophoresis was used to analyze the patterns of phosphoproteins obtained in in vitro protein kinase assays with the cell-free lysates of wild type as well as all mutant strains. Detailed record
	Vztah mezi modulární strukturou serin/threonin proteinkinasy eukaryontního typu StkP z Streptococcus pneumoniae a její funkcí Pallová, Petra ; Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel In order to determine a cellular localization of protein kinase StkP we prepared its epitope-labelled form expressed in S. pneumoniae. The full-length form of StkP was detected with monoclonal antibody in the membrane fraction. In vitro autophosphorylation assays confirmed this finding and phosphorylated product of corresponding mass was detected in the membranes. These results clearly showed that StkP is a membrane-associated protein. Detailed record
	Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel To identify the genes whose expression is controlled by protein kinase StkP we examined the transcription profile of DstkP mutant by DNA microarray technology. The transcript analysis revealed the StkP-dependent expression of many potential target loci. Out of 2,300 genes evaluated, 49 were found to be specifically and strongly upregulated in the DstkP strain, whereas 48 were downregulated. The data obtained from transcription profile were further extended by proteomic studies and mass spectrometry. Detailed record
	Ligninolytické enzymy rodu Trametes Tomšovský, Michal ; Homolka, Ladislav Eighty-four strains belonging to four species of the genus Trametes Fr. (T. versicolor, T. hirsuta, T. ochracea and T. cervina) collected in different localities and on different substrates were screened for laccase production and eight of them with their monokaryotic derivates were then examined in more detail for their ability to produce also other lignin-modifying enzymes (manganese peroxidase and lignin peroxidase). Production variability was relatively high and no significant correlation was found between the origin of the strains (locality, substrate) and the enzyme production. Dikaryons of all three species (but not of all their strains) exhibited LiP activity, which was not detected in the respective monokaryons. Detailed record
	Je EF-TU, jako dobře známý bakteriální molekulární přepínač, stále hádankou pro strukturní biology? Holub, Martin ; Weiser, Jaroslav We purified EF-Tu from two closely related strains of Streptomyces aureofaciens and used them in comparative studies in order to better understand the structural and functional features of their aggregation. Detailed record

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