Original title: Characterization of putative methyltransferase MT420 in \kur{Trypanosoma brucei.}
Authors: PROCHÁZKOVÁ, Michaela
Document type: Master’s theses
Year: 2010
Language: eng
Abstract: [eng] [cze]
Localization and characterization of putative mitochondrial methzltransferase acc. No.: Tb10.6k15.0440 in Trypanosoma brucei was performed. Employed molecular methods included immunofluorescence, sub-cellular fractionation and tandem affinity purification. Protein was overexpressed in an E. coli expression system, using an in-fusion expression vector pOPINM with maltose binding protei (MBP) tag. Localization and characterization of putative mitochondrial methzltransferase acc. No.: Tb10.6k15.0440 in Trypanosoma brucei was performed. Employed molecular methods included immunofluorescence, sub-cellular fractionation and tandem affinity purification. Protein was overexpressed in an E. coli expression system, using an in-fusion expression vector pOPINM with maltose binding protei (MBP) tag.
Keywords: localization; MBP fusion protein; mitochondrion; SAM-dependent methyltransferase; translation release factor
Citation: PROCHÁZKOVÁ, Michaela. Characterization of putative methyltransferase MT420 in \kur{Trypanosoma brucei.}. České Budějovice, 2010. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/14354

Permalink: http://www.nusl.cz/ntk/nusl-80270


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses