Original title:
Izolace, charakterizace a lokalizace ortologní sekvence genu \kur{Notch} u obaleče jablečného, \kur{Cydia pomonella}
Translated title:
Isolation, characterization and localization of orthologous sequence of the \kur{Drosophila Notch} gene in codling moth, \kur{Cydia pomonella}
Authors:
KŮTA, Václav Document type: Master’s theses
Year:
2011
Language:
cze Abstract:
The codling moth, Cydia pomonella (Tortricidae) is a significant pest in apple orchards. In the 1990ies, a control programme using the Sterile Insect Technique (SIT) has been established, based on bisexual releases of sterile insects into wild populations of this pest. Male-only releases are not possible due to the lack of an efficient system to produce male-only progeny. Recently, a new approach has been proposed for the development of genetic sexing strains in Lepidoptera. It is based on insertion of a dominant conditional lethal mutation of the Notch gene, derived from the N60g11 allele of Drosophila, into the female W chromosome by means of transgenesis. This study deals with isolation, characterization, and chromosomal localization of a codling moth orthologue of the Drosophila Notch gene with the aim to prepare a mutant sequence of the orthologue to be used in plasmid constructs for germline transformation of this pest.
Keywords:
codling moth; genetic sexing strain; transgenosis; genetická separace pohlaví; obaleč jablečný; transgenoze Citation: KŮTA, Václav. Izolace, charakterizace a lokalizace ortologní sekvence genu \kur{Notch} u obaleče jablečného, \kur{Cydia pomonella}. České Budějovice, 2011. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/14290