Original title: Structural and Functional Study on Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin Receptor (TRPA1) Channels
Translated title: Structural and Functional Study on Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin Receptor (TRPA1) Channels
Authors: SAMAD, Abdul
Document type: Doctoral theses
Year: 2010
Language: cze
Abstract: Investigations of structural and functional relationships of rat transient receptor potential cation channel, subfamily V, member 1 (TRPV1), also known as the capsaicin receptor, and human transient receptor potential cation channel, subfamily A, member 1, also known as TRPA1, are presented. Capsaicin induced Ca2+ -dependent desensitization of rat TRPV1 channel is studied and lead to the identification of key amino acid residues in the C- terminal domain of TRPV1 interacting with the membrane phospholipid PIP2 and an intradomain interaction that controls the open and desensitized state of the TRPV1 channel. Further the molecular basis of agonist AITC- and voltage-dependent gating on TRPA1 is explained. Hereby, residue P949 located near the center of the sixth transmembrane spanning helix (S6) is structurally required for normal functioning of the receptor and the distal bi-glycine G958XXXG962 motif controls its activation/deactivation properties. Furthermore, the gating region is extended towards the cytoplasmic part of the channel, putatively located near the inner mouth of the channel pore. A following series of experiments lead to the identification of a limited number of residues that appear important for allosteric regulation of the channel by chemical and voltage stimuli (K969, R975, K989, K1009, K1046, K1071, K1092 and K1099). In addition, three charge-neutralizing `gain-of- function{\crq} mutants (R975A, K988A, and K989A) which exhibited higher sensitivity to depolarizing voltages were characterized, indicating that these residues are directly involved in voltage-dependent modulation of TRPA1.
Keywords: electrophysiology; molecular biology; protein structure and function; Transient receptor potential channels; TRPA1; TRPV1; electrophysiology; molecular biology; protein structure and function; Transient receptor potential channels; TRPA1; TRPV1
Citation: SAMAD, Abdul. Structural and Functional Study on Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin Receptor (TRPA1) Channels. České Budějovice, 2010. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/12148

Permalink: http://www.nusl.cz/ntk/nusl-79765


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Doctoral theses