Original title:
Community assembly and population genetic structure of moths along elevational gradient in a tropical rainforest
Authors:
IBALIM, Sentiko Document type: Doctoral theses
Year:
2025
Language:
eng Abstract:
This Thesis examines the community assembly and population genetic structure of moth communities along Mt. Wilhelm elevational gradient (50-3700 m a.s.l) in Papua New Guinea. The Thesis utilizes an extensively sampled DNA molecular barcode data (mtDNA-CO1) obtained through barcoding and metabarcoding techniques. First, we retrieved phylogenetic structure of the moth family Geometridae and used this to assess how abiotic and biotic factors influence the community assembly. We then expanded this study to determine the diversity trends of all moth families using a metabarcoding approach, making this the first ever large-scale survey of Lepidoptera in the region. We recorded nine common superfamilies, 49 families, 893 genera and 2,322 species from 11.5 million metabarcode reads. We described their diversity trends at super familial and familial levels and assessed alpha and beta diversity trends for species with increasing elevation. Finally, we explored the population structure of four common and widespread moth species sequenced through metabarcoding. We used haplotype networks and the FST fixation indexes to investigate gene flow among nine surveyed moth communities and their historical population demographics through Bayesian Skyline Plots (BSP) and infer the implications for conservation.
Keywords:
Altitudinal-gradients; barcode; genetic diversity; Lepidoptera; metabarcoding; moths; Mt. Wilhelm; Papua New Guinea; Phylogenetic-structure Citation: IBALIM, Sentiko. Community assembly and population genetic structure of moths along elevational gradient in a tropical rainforest. České Budějovice, 2025. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/77683