Original title: Stable isotope characterization of fungal water and inorganic carbon assimilation signals and ecosystem fluxes
Authors: JABINSKI, Stanislav
Document type: Doctoral theses
Year: 2024
Language: eng
Abstract: Fungi are a group of major decomposers in soils and therefore play a key role in the biogeochemical cycles in environmental systems. To get a better understanding of their metabolic role, we extended a dual stable isotope probing assay which applies labeled bicarbonate as inorganic C and heavy water (2H2O). The advantages of this approach are that both are overly abundant in the environment and therefore do not disturb or add nutrients into natural systems. Only little information could be found in the literature regarding the potential of inorganic carbon assimilation by fungi and no information could be found for the hydrogen water assimilation. Therefore, this PhD was aimed to investigate the isotopic signatures of a diverse phylogenetic range of saprotrophic fungi to explore the variations in the fungi when grown under different substrate classes before applying the obtained knowledge to incubations of natural systems. To fully apply this assay potential, we investigated under laboratory conditions the potential of assimilation of inorganic C and the hydrogen water assimilation into the membrane lipid biomarkers of fungi to distinguish differences in metabolic modes specifically targeting the difference in growth on labile vs more complex C sources.
Keywords: heterotrophic CO2 fixation; hydrogen isotopes; Lipid-SIP; microbial fatty acids; microbial production; Soil incubation
Citation: JABINSKI, Stanislav. Stable isotope characterization of fungal water and inorganic carbon assimilation signals and ecosystem fluxes. České Budějovice, 2024. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/59346

Permalink: http://www.nusl.cz/ntk/nusl-668942


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Doctoral theses