Využití kapilární elektroforézy pro analýzu liposomů a studium interakcí s léčivy

Šimonová, Alice

(EN) The first part of this dissertation thesis investigates the possibilities of analysis and characterization of liposomes using capillary electrophoresis. Our primary objectives were to identify suitable background electrolytes and optimize experimental conditions to minimize liposome adsorption onto the capillary wall. To overcome the limitations of the UV detection method, we incorporated fluorescently labeled phosphatidylcholine into our liposomal formulation which allowed us to distinguish between fluorescently labeled liposomes and other compounds. By utilizing laser-induced fluorescence detection, we proved that liposome adsorption onto the capillary wall was occurring. The second part is focused on the dynamic and permanent coating of the capillary wall to overcome problems with liposome adsorption. Several dynamic coating approaches were tested for four different polymer coating agents and evaluated based on the suppression of the electroosmotic flow and the coating stability. Linear polyacrylamide was used for permanent coating and its performance was compared with the tested dynamic coatings. This part utilized capillary electrophoresis with laser-induced fluorescence detection to enhance the sensitivity and specificity of liposome detection. Lastly, liposomes were used as a...

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