Original title: Exploring Inter-subunit contact sites within the Hexameric Orai channel
Authors: ECHEFU, Linda Victoria
Document type: Master’s theses
Year: 2024
Language: eng
Abstract: This study explores potential inter-subunit interactions of the hexameric Orai1 channel. Our investigation include the loss of function (LoF) mutants Orai1 K85E and E149K residues. We demonstrated that the Orai1 K85E mutation reduces homomerization via fluorescence resonance energy transfer (FRET) measurements, while the homomerization of E149k remains comparable to the Orai1 wild type In addition, we identified spatial proximity of Orai1 V181 and C143 by implementing the proximity-dependent chemical crosslinking unnatural amino acid (UAA) O-(2-bromoethyl)-tyrosine (BrEtY). Overall, we provide information about the structural assembly of the Orai1 channel.
Keywords: Ca2+; CETR; CRAC channel; dimers; gating; Hexameric; ion channel; Mock; monomers Orai pore; MTR; Orai1; SOCE; TM(Transmembrane); TM1; TM2; TM3; TM4; WT
Citation: ECHEFU, Linda Victoria. Exploring Inter-subunit contact sites within the Hexameric Orai channel. České Budějovice, 2024. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/74616

Permalink: http://www.nusl.cz/ntk/nusl-668429


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses