Original title: Kininase-like metalloprotease regulates tick salivation
Authors: FOITLOVÁ, Denisa
Document type: Master’s theses
Year: 2024
Language: eng
Abstract: Ticks are obligate hematophagous ectoparasites that require a blood meal to complete their development and reproduction. To facilitate blood feeding, they secrete saliva, a complex mixture of bioactive compounds that modulate the host's immune responses. To evade host immunity, ticks have evolved their salivary proteins to exhibit low immunogenicity. However, in some less adapted hosts, compounds of tick saliva can evoke antibody-mediated responses. Angiotensin-converting enzyme-like kininase (Ir-ACE), the main subject of this thesis, was identified as one of the most dominant salivary antigens of the tick Ixodes ricinus. The aim of this thesis was to investigate the molecular and functional properties of this enzyme; an objective that has not yet been accomplished. To achieve this, RNAi and repeated feeding experiments, proteomic analysis of salivary glands and expression of recombinant Ir-ACE used for the vaccination experiments and substrate profiling were performed.
Keywords: angiotensin-converting enzyme-like kininase; metalloproteases; saliva; salivary glands; ticks
Citation: FOITLOVÁ, Denisa. Kininase-like metalloprotease regulates tick salivation. České Budějovice, 2024. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/67667

Permalink: http://www.nusl.cz/ntk/nusl-623884


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses