Original title: The Overlooked Fact: the Critical Role of Bioinformatics Pipeline in Microbiome Analysis
Authors: Polakovičová, Petra
Document type: Papers
Language: eng
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: The research on the human microbiome has gained significant popularity over the past couple of years across both scientific and public communities. This area has revealed considerable knowledge, mainly thanks to next-generation sequencing. Even though technology possibilities evolve faster than ever, and there is an enormous amount of publicly available data, there are not yet precise procedures, methods, and bioinformatics tools to work accurately enough with microbiome data, and there are no standardized recommendations for bioinformatics pipelines to assess the most accurate results. Here, we demonstrate the potential inaccuracies that can arise from various bioinformatics processing of amplicon 16S RNA sequencing data by performing a comparative analysis of diverse pipeline configurations using mock community samples and biological material originating from biopsy samples obtained during regular colonoscopy from patients with primary sclerosing cholangitis.
Keywords: 16S rRNA; bioinformatics; comparative analysis; microbiome
Host item entry: Proceedings I of the 30st Conference STUDENT EEICT 2024: General papers, ISBN 978-80-214-6231-1, ISSN 2788-1334

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: https://hdl.handle.net/11012/249206

Permalink: http://www.nusl.cz/ntk/nusl-622597


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