Original title: Quantitative analysis of Scratch assay and Colony formation assay data using MATLAB
Authors: Ingrová, Kateřina ; Chmelíková, Larisa ; Zumberg, Inna
Document type: Papers
Language: eng
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: Utilizing Scratch and Colony formation assays is crucial for understanding cell migration and proliferation dynamics. In this study, we present novel approaches utilizing MATLAB for the processing of images obtained from these assays. We demonstrate the effectiveness of our approaches through validation experiments on human osteosarcoma SaOS-2 cells, showcasing their applicability across different experimental conditions. Moreover, the inhibitory effect of two concentrations of gentamicin (0.1 mg/ml, 0.3 mg/ml) on the migration and clonogenicity of SaOS-2 cells was declared.
Keywords: Colony formation assay; Gentamicin; MATLAB; SaOS-2; Scratch assay
Host item entry: Proceedings II of the 30st Conference STUDENT EEICT 2024: Selected papers, ISBN 978-80-214-6230-4, ISSN 2788-1334

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: https://hdl.handle.net/11012/249281

Permalink: http://www.nusl.cz/ntk/nusl-622578


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